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PubMed · 11948855

Artificial nucleases.

C B Chen·L Milne·R Landgraf·D M Perrin·D S Sigman
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Abstract

The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).

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Base SequenceDNADNA-Binding ProteinsDeoxyribonucleasesEnzyme InhibitorsHydrogen PeroxideMacromolecular SubstancesModels, MolecularMolecular ConformationOxidation-ReductionPhenanthrolinesPhosphatesSubstrate SpecificityTranscription, GeneticJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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C B Chen, L Milne, R Landgraf, D M Perrin, D S Sigman. 2001-10-01. Artificial nucleases.. https://doi.org/10.1002/1439-7633(20011001)2%3A10%3C735%3A%3Aaid-cbic735%3E3.0.co%3B2-%23

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