PubMed · 11885141
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
Kiyokazu Ikeda. 2002. Buckwheat: composition, chemistry, and processing.. https://doi.org/10.1016/s1043-4526(02)44008-9
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Different explants and compounding proportions of different hormones were comparatively studied in tissue culture of Fagopyrum esculentum Moench and thereafter an efficient plant-regeneration system was established by in vitro F. esculentum Moench culture. On MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA, 89.6% cotyledon segments could be induced to produce calli; however, on MS medium containing 2.0 mg/L 2,4-D and 1.0-2.0 mg/L 6-BA, the induction rate of hypocotyl segments could reach as high as 100%. On MS medium containing 2.0 mg/L 6-BA, 0.1 mg/L IAA and 1 mg/L KT, adventitious buds could be regenerated indirectly from calli or directly from explants, the differentiation rates of cotyledon-derived calli and hypocotyl-derived calli were 42.5% and 73.6% respectively; the calli coming from hypocotyl segments differentiated evidently at a higher rate than the calli originated from cotyledon segments. Well-grown adventitious buds were inoculated on 1/2 MS medium containing 1.0 mg/L IBA and 0.5 mg/L NAA, 100% rooting frequency was obtained. Plantlets grew well and appeared normal with no mortality after being transplanted to soil. Moreover, the survival rate of plantlets reached 91.6%.
To investigate the effects of changes in lipase, lipoxygenase, peroxidase (POX), and rutin concentrations on the quality of buckwheat flour, 14 buckwheat varieties were stored for 0, 4, 10, and 30 days at 5 or 20 degrees C. During the storage period, lipase activity correlated to pH (significantly negative) and water-soluble acid (WSA) (significantly positive). The lipoxygenase 1 protein concentration had a negative correlation to WSA (significant at 0 and 4 storage days at 5 degrees C and at 0 and 10 storage days at 20 degrees C). POX had significant correlation to pH and peroxide value (POV) at 5 degrees C, whereas it was not significant at 20 degrees C. The rutin concentration had negative correlations to WSA (significant at 30 days of storage at 5 degrees C and at 4 days of storage at 20 degrees C). Thus, lipase activity plays an important role that relates to lipid degradation in quality deterioration of buckwheat flour.
We assessed the genetic diversity in Japanese indigenous common buckwheat (Fagopyrum esculentum) cultivars using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers and investigated the relationships between the genetic diversity and agronomic traits. The average expected intracultivar hetero zygosity was 0.303 for AFLP and 0.819 for SSR. The differentiations among agroecotypes, among cultivars within an agroecotype, and among cultivars were small (0.002, 0.024, and 0.026 for SSR and 0.013, 0.013, and 0.026 for AFLP, respectively) but statistically significant from zero except for the SSR differentiation among agroecotypes. In principal coordinates analysis, cultivars within the same agroecotype tended to cluster, indicating that agroecotypes well reflected the genetic relationships among cultivars. In AFLP, the differentiation among the agroecotypes was more distinct than in SSR, and genetic distance showed a moderate correlation with the difference in quantitative traits, indicating that AFLP can resolve the relationships among cultivars with better resolution than SSR. By contrast, SSR may be more sensitive to demographic changes. Four of the five SSR markers showed a significant positive correlation (Kendall's tau = 0.382-0.607) between allelic richness and variation in flowering timing, indicating that cumulative bottleneck events have occurred during the population history, with a decline in the variation of photosensitivity of flowering.