PubMed · 11808152
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
K Dan. 2001. [Aplastic anemia].. https://pubmed.ncbi.nlm.nih.gov/11808152/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Explore the source record for details and available documents.
The present study investigated whether anabolic androgenic steroid (AAS) treatment (daily subcutaneous injections during 2 weeks with nandrolone decanoate; 15 mg/kg) affects competitive behaviour, and locomotor activity response to a sedative dose of ethanol (0.5 g ethanol/kg). In addition, levels of brain monoamines were assessed. The results showed that AAS treated animals exhibited enhanced dominant behaviour in the competition test compared to controls. The AAS groups' locomotor activity was not affected by ethanol in contrast to the controls who showed a sedative locomotor activity. AAS animals had significant lower levels of serotonin in basal forebrain and dorsal striatum compared to controls. These findings further strengthen the fact that AAS affects behaviour, as well as biochemical parameters. Based on previous studies and results from the present study, we hypothesize that AAS abuse may constitute a risk factor for disinhibitory behaviour, partly by affecting the serotonergic system.
Detection of anabolic steroids in animal urine samples is currently performed with GC-MS in our lab. However we found that the detection of 17 alpha-trenbolone (17 alpha-TbOH), 4-chloroandrost-4-ene-3,17-dion (CLAD), 16- beta -OH-stanozolol (16OHstan) and alpha- and beta-boldenone (alpha -Bol, beta -Bol) was very difficult, if not impossible. Therefore a sensitive, specific and selective qualitative multi-analyte LC-MS-MS method was developed. The LC separation was achieved by using a Symmetry C(18) column and methanol-water-formic acid (54.7-44.7-0.6) as a mobile phase at a flow-rate of 0.3 ml/min. The mass spectrometer was operated in multiple reaction monitoring mode with positive electrospray interface. Validation of the method was done according to draft SANCO/1805/2000 Rev.1 and a CC beta smaller then 1 ng/ml was obtained for each compound.