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PubMed · 11791240

DNAinsight: a web based image processing system for large scale RLGS analysis.

K Takahashi·M Nakazawa·Y Watanabe
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Abstract

DNA methylation occurring within the context of CpG site in the promoter element generally correlates with transcriptional silencing, delayed replication, condensed chromatin and mammalian genome imprinting (Nature Genet 24:101-2, 2000). We have been focusing attention on Restriction Landmark Genomic Scanning (RLGS) method as the powerful experimental procedure which can used to determine DNA methylation status change occurring on a genome simultaneously. To support systematic analysis with RLGS method, we have developed the automated processing algorithms for two-dimensional electrophoretograms of genomic DNA based on RLGS method (RLGS profile). Our powerful processing algorithms realize the automated spot recognition from RLGS profiles and the automated comparison of a huge number of such profiles. To make systematic RLGS analysis more easy and objective, we equipped our automated RLGS image processing algorithms with a WWW interface and relational database which stores the RLGS profile images together with their accompanying information, such as DNA sample specification, two-dimensional electrophoresis conditions and so forth. The web based RLGS image processing system "DNAinsight" can be accessed via URL http://www.methylome.jp/DNAinsight/.

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AlgorithmsComputational BiologyCpG IslandsDNADNA MethylationDatabases, Nucleic AcidElectrophoresis, Gel, Two-DimensionalGenome, HumanGenomicsHumansImage Processing, Computer-AssistedInternetJournal Article

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K Takahashi, M Nakazawa, Y Watanabe. 2001. DNAinsight: a web based image processing system for large scale RLGS analysis.. https://pubmed.ncbi.nlm.nih.gov/11791240/

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