PubMed · 11548374
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S Forrest. 2001. Microbicides now.. https://pubmed.ncbi.nlm.nih.gov/11548374/
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Bronopol (2-bromo-2-nitropropane-1,3-diol) is widely used as an anti-microbial in cosmetics, external medicaments, shampoos and bath preparations. A reversed-phase high performance liquid chromatographic separation method with UV spectrophotometric detection was developed for the determination of bronopol and its degradation products. Degradation of bronopol was observed in aqueous medium, giving rise to non-reproducible data. To overcome this problem, HPLC grade methanol (water content <0.05%) was used for sample preparation. In addition, the parameters for the HPLC analysis of bronopol were optimised. It was found that bronopol standards and product samples were stable in methanol for at least 1 month at ambient temperature, thus allowing a quantitative and reproducible determination of bronopol.
A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method was developed for the assay of enrofloxacin in raw material and injection. The validation method yielded good results and included the range, linearity, precision, accuracy, specificity, recovery, limit of detection (LOD) and limit quantification (LOQ) values. The HPLC separation was carried out by reversed phase chromatography on a C-18 absorbosphere column (150 x 4.6 mm i.d. 5 microm particle size) with a phase composed of sodium acetate (pH 4.7; 0.1 M): acetonitrile (60:40, v/v; pH 5.0), pumped isocratically at a flow rate of 1.5 ml min(-1). The effluent was monitored at 278 nm with the eluting solvent. The calibration graph for enrofloxacin was linear from 10.0 to 80.0 microg ml(-1).
A simple and rapid capillary electrophoresis method with UV detection was developed and validated for the determination of rufloxacin hydrochloride in coated tablets. An experimental design strategy (Doehlert design and desirability function) allowed the analytical parameters to be simultaneously optimized in order to determine rufloxacin hydrochloride with high peak area/migration time ratio, good efficiency and short analysis time. Optimized analyses were run using boric acid 0.10 M adjusted to pH 8.8 as BGE and setting voltage and temperature at 18 kV and 27 degrees C, respectively. Pefloxacin mesylate was used as internal standard and run time was about three minutes. The method was validated for the drug substance and the drug product according to the ICH3 guidelines. Robustness was tested by experimental design using an eight-run Plackett-Burman matrix.