High-speed solution switching using piezo-based micropositioning stages.

S Stilson; A McClellan; S Devasia

doi:10.1109/10.930905

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High-speed solution switching using piezo-based micropositioning stages.

S Stilson·A McClellan·S Devasia

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Abstract

Motion-induced vibration is a critical limitation in high-speed micropositioning stages used to achieve solution switching. Controlled rapid solution switching is used to study the fast activation and deactivation kinetics of ligand-gated ion-channel populations isolated in excised membrane patches--such studies are needed to understand fundamental mechanisms that mediate synaptic excitation and inhibition in the central nervous system. However, as the solution-switching speed is increased, vibration induced in the piezo-based positioning stages can result in undesired, repeated, ligand application to the excised patch. The article describes a method to use knowledge of the piezo-stage's vibrational dynamics to compensate for and reduce these unwanted vibrations. The method was experimentally verified using an open-electrode technique, and fast solution switching (100 micros range) was achieved.

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Models, Neurological Patch-Clamp Techniques Signal Processing, Computer-Assisted Vibration Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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S Stilson, A McClellan, S Devasia. 2001. High-speed solution switching using piezo-based micropositioning stages.. https://doi.org/10.1109/10.930905

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