Enzymatic computing.

K P Zauner; M Conrad

doi:10.1021/bp010004n

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PubMed · 11386878

Enzymatic computing.

K P Zauner·M Conrad

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Abstract

The conformational dynamics of enzymes is a computational resource that fuses milieu signals in a nonlinear fashion. Response surface methodology can be used to elicit computational functionality from enzyme dynamics. We constructed a tabletop prototype to implement enzymatic signal processing in a device context and employed it in conjunction with malate dehydrogenase to perform the linearly inseparable exclusive-or operation. This shows that proteins can execute signal processing operations that are more complex than those performed by individual threshold elements. We view the experiments reported, though restricted to the two-variable case, as a stepping stone to computational networks that utilize the precise reproducibility of proteins, and the concomitant reproducibility of their nonlinear dynamics, to implement complex pattern transformations.

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Calcium Enzymes Image Processing, Computer-Assisted Magnesium Malate Dehydrogenase Models, Chemical Models, Molecular Osmolar Concentration Protein Conformation Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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K P Zauner, M Conrad. Enzymatic computing.. https://doi.org/10.1021/bp010004n

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