Human mast cell transcriptome project.

H Saito; T Nakajima; K Matsumoto

doi:10.1159/000053790

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Human mast cell transcriptome project.

H Saito·T Nakajima·K Matsumoto

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Abstract

After draft reading of the human genome sequence, systemic analysis of the transcriptome (the whole transcripts present in a cell) is progressing especially in commonly available cell types. Until recently, human mast cells were not commonly available. We have succeeded to generate a substantial number of human mast cells from umbilical cord blood and from adult peripheral blood progenitors. Then, we have examined messenger RNA selectively transcribed in these mast cells using high-density oligonucleotide probe arrays. Many unexpected but important transcripts were selectively expressed in human mast cells. We discuss the results obtained from transcriptome screening by introducing our data regarding mast-cell-specific genes.

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Blood Cells Fetal Blood Gene Expression Profiling Human Genome Project Humans Hypersensitivity Mast Cells Oligonucleotide Array Sequence Analysis RNA, Messenger Umbilical Veins Comparative Study Journal Article Review

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H Saito, T Nakajima, K Matsumoto. 2001. Human mast cell transcriptome project.. https://doi.org/10.1159/000053790

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