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PubMed · 11257533

Highly efficient single molecule detection in microstructures.

K Dörre·J Stephan·M Lapczyna·M Stuke·H Dunkel·M Eigen
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Abstract

For DNA single molecule sequencing, the complete detection of all dye-labeled monomers which are cleaved off during the sequencing reaction is an essential requirement. In this work we address the feasibility of single molecule detection in microstructures with a confocal multi element set-up. We present statistical data on single molecule recognition and explain a refined data evaluation technique for single molecule burst analysis. From these data the signal-to-noise ratio in microstructures is evaluated as well as the overall detection efficiency. So far, detection efficiencies of single molecule events of up to 60% have been shown in microstructures.

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BiochemistryFluorescent DyesImage Processing, Computer-AssistedPolymethyl MethacrylateSpectrometry, FluorescenceEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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K Dörre, J Stephan, M Lapczyna, M Stuke, H Dunkel, M Eigen. 2001-04-13. Highly efficient single molecule detection in microstructures.. https://doi.org/10.1016/s0168-1656(00)00415-6

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