Decrease in immune activation in HIV-infected patients treated with highly active antiretroviral therapy correlates with the function of hematopoietic progenitor cells and the number of naive CD4+ cells.

S D Nielsen; T U Sørensen; A K Ersbøll; N Ngo; L Mathiesen; J O Nielsen; J E Hansen

doi:10.1080/003655400459487

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Decrease in immune activation in HIV-infected patients treated with highly active antiretroviral therapy correlates with the function of hematopoietic progenitor cells and the number of naive CD4+ cells.

S D Nielsen·T U Sørensen·A K Ersbøll·N Ngo·L Mathiesen·J O Nielsen·J E Hansen

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Abstract

This study was conducted to determine the impact of immune activation, cytokine production and apoptosis on the naive CD4+ cell count and the function of hematopoietic progenitor cells during the initial phase of highly active antiretroviral therapy (HAART). Blood samples from 11 HIV-infected patients were collected prior to HAART and after 4 and 12 weeks of therapy. Flow cytometry was used to determine the naive CD4+ count and activated T cells. The cloning efficiency of progenitor cells was determined using a colony-forming cells assay. Finally, apoptosis and cytokine production were determined. During the study period, the naive CD4+ count and the cloning efficiency increased significantly. Immune activation was found in HIV-infected patients and decreased during HAART. The level of immune activation correlated negatively with both the naive CD4+ count and the function of progenitor cells. A negative correlation was found between apoptosis and the naive CD4+ count. Alterations in cytokine production during HAART or correlation between cytokine production and the naive CD4+ count or the cloning efficiency of progenitor cells were not detected. In conclusion, immune activation in HIV-infected patients treated with HAART is inversely correlated with the function of progenitor cells and the naive CD4+ count.

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Anti-HIV Agents Antiretroviral Therapy, Highly Active Apoptosis CD4 Antigens CD4 Lymphocyte Count CD4-Positive T-Lymphocytes Cytokines Flow Cytometry HIV Infections Hematopoietic Stem Cells Humans Male Time Factors Viral Load Clinical Trial Journal Article Randomized Controlled Trial Research Support, Non-U.S. Gov't

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S D Nielsen, T U Sørensen, A K Ersbøll, N Ngo, L Mathiesen, J O Nielsen, J E Hansen. 2000. Decrease in immune activation in HIV-infected patients treated with highly active antiretroviral therapy correlates with the function of hematopoietic progenitor cells and the number of naive CD4+ cells.. https://doi.org/10.1080/003655400459487

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