PubMed · 11022545
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M Childs. 2000. My privilege.. https://pubmed.ncbi.nlm.nih.gov/11022545/
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Glutathione S-transferase (GST) mu phenotype was assessed in colon tissue from patients with ulcerative colitis and colorectal neoplasms that were positive for GSTM1 genotype. GST mu protein (enzyme linked immunosorbent assay) was absent in 2/9 unaffected colon tissue (22.3%), 4/13 tissues with chronic ulcerative colitis (CUC) (30.7%), 4/11 adenomas (36.4%) and 7/14 adenocarcinomas (50.0%; P<or=0.05). GST-3H-trans-stilbene oxide activity was detected in all tissues except 1/13 (7.6%) CUC tissues and 7/14 (50.0%, P<or=0.05) colorectal adenocarcinomas. Immunoreactive GST mu was observed in colonic epithelial cells but not in adjacent neoplastic cells by immunohistochemistry. Two-dimensional electrophoresis revealed several mu class isoforms in cytosol from unaffected colon that were absent in matched tumor cytosol. These results indicate that GSTM1 genotype may not necessarily reflect GST mu phenotype in colorectal tumors.
The widespread consumption of tea as beverage throughout the world has stimulated interest in the possibility of its use in chemoprevention of cancer. The present set of investigation was carried out to evaluate the anticarcinogenic activity of black tea using lung tumorigenesis model in Swiss albino mice. Diethylnitrosoamine (DEN), a known inducer of pulmonary tumors was given at the multiple (total eight) doses of 20mg/kg body weight through oral intubation to Swiss albino mice. Simultaneously, three different groups of animals received 1, 2 and 4% aqueous black tea extracts (ATE) as a sole source of drinking solution. The positive control group received DEN treatment only. The animals were sacrificed after 28 weeks of the first dose of DEN. They were identified for different histological types of alveologenic pulmonary tumors. In the positive DEN treated group, higher incidences of pulmonary tumors were observed, while in ATE treated groups, a lower incidence of DEN induced lung tumorigenicity was recorded. The percentage of mice having lung tumors was decreased following ATE administration. Besides this, significant decrease in the number of tumors/mouse was observed in 2 and 4% ATE supplemented groups. The histological examination revealed a significant decrease in pulmonary adenomas at all doses of ATE. The number of animals showing pulmonary adenocarcinomas induced by DEN was found to be inhibited significantly by 4% ATE. In addition, splenomegaly was found to be protected by ATE administration.
Our demonstration of a 19kDa anti-Kaposi's sarcoma (KS) ribonuclease (RNase) in urine from a non-pregnant female may provide at least part of the explanation for the low incidence of KS in human females. N-terminal sequence analysis and isoelectric focusing of the purified RNase, coupled with the very low levels of anti-KS activity noted for recombinant forms of human eosinophil derived neurotoxin and human eosinophil cationic protein, suggest that the 19kDa enzyme is an eosinophilic protein whose potent anti-KS activity is dependent upon post-translational modifications that occur only in human cells.