PubMed · 11006532
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G R Nakayama. 2000. Web alert.. https://doi.org/10.1016/s1367-5931(00)00119-8
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The applicability and usefulness of electrospray mass spectrometry to the analysis of a small data set of 52 synthetic substances to probe their molecular similarity/diversity has been demonstrated. The first stage was the reduction of the data by Principal Component Analysis (PCA), to visualize the structure of the data. Sequential Projection Pursuit (SPP) was applied to detect outlying objects. Hierarchical cluster analysis was employed to produce a dendrogram, using group-average linkage clustering. Finally, the cluster results of spectral data were compared with that of structural fingerprints and an expert's classification by using the similarity measure of Wallace.
Gravimetric lipid determination is a major parameter for the characterization and the authentication of royal jelly quality. A solid/liquid extraction was compared to the reference method, which is based on liquid/liquid extraction. The amount of royal jelly and the time of the extraction were optimized in comparison to the reference method. Boiling/rinsing ratio and spread of royal jelly onto the extraction thimble were identified as critical parameters, resulting in good accuracy and precision for the alternative method. Comparison of reproducibility and repeatability of both methods associated with gas chromatographic analysis of the composition of the extracted lipids showed no differences between the two methods. As the intra-laboratory validation tests were comparable to the reference method, while offering rapidity and a decrease in amount of solvent used, it was concluded that the proposed method should be used with no modification of quality criteria and norms established for royal jelly characterization.
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