Printing proteins as microarrays for high-throughput function determination.

G MacBeath; S L Schreiber

doi:10.1126/science.289.5485.1760

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PubMed · 10976071

Printing proteins as microarrays for high-throughput function determination.

G MacBeath·S L Schreiber

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Abstract

Systematic efforts are currently under way to construct defined sets of cloned genes for high-throughput expression and purification of recombinant proteins. To facilitate subsequent studies of protein function, we have developed miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins. A high-precision robot designed to manufacture complementary DNA microarrays was used to spot proteins onto chemically derivatized glass slides at extremely high spatial densities. The proteins attached covalently to the slide surface yet retained their ability to interact specifically with other proteins, or with small molecules, in solution. Three applications for protein microarrays were demonstrated: screening for protein-protein interactions, identifying the substrates of protein kinases, and identifying the protein targets of small molecules.

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Biochemistry Biotin Digoxigenin Fluorescence Fluorescent Dyes Ligands Molecular Probe Techniques Phosphorylation Piperazines Protein Binding Protein Folding Protein Kinases Proteins Robotics Serum Albumin, Bovine Journal Article Research Support, Non-U.S. Gov't

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G MacBeath, S L Schreiber. 2000-09-08. Printing proteins as microarrays for high-throughput function determination.. https://doi.org/10.1126/science.289.5485.1760

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