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PubMed · 10885563

CDw108 expression during T-cell development.

T Mine·K Harada·T Matsumoto·H Yamana·K Shirouzu·K Itoh·A Yamada
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Abstract

We recently reported a gene encoding the human CDw108, a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that is preferentially expressed on activated T lymphocytes and erythrocytes. The present study investigated the expression of CDw108 on various tissues and cells, particularly on T cells during development. The murine CDw108 cDNA was cloned initially, and it was highly homologous to the human CDw108 (88.0% or 89.3% similarity at the nucleotide or amino acid level, respectively) or identical to the murine semaphorin K1/Sema7A. The CDw108 mRNA was demonstrated in a few tissues including thymus and brain with the highest expression coming on day 7 in whole embryo followed by relatively consistent expression during development. Cell-surface expression of the CDw108 during T-cell development was further examined by flow cytometry in the human umbilical cord blood and thymus. It was preferentially expressed on a CD34+ stem cell population of umbilical cord blood, and CD3dull CD34+/- CD117 (c-kit)+ CD4bright CDbright cells in the thymus that are involved in the stage of positive selection. These results suggest the contribution of CDw108 in T-cell development, especially in the stage of positive selection in the thymus.

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Age FactorsAnimalsAnimals, NewbornAntigens, CDBlotting, NorthernBrain ChemistryCloning, MolecularDNA, ComplementaryFemaleFetal BloodFlow CytometryGPI-Linked ProteinsGene Expression Regulation, DevelopmentalGlycoproteinsHumansLipoproteinsMaleMiceMice, Inbred ICRMolecular Sequence DataPlacentaPregnancyRNA, MessengerSemaphorinsSequence Homology, Amino AcidSpleenT-LymphocytesTestisThymus GlandJournal Article

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T Mine, K Harada, T Matsumoto, H Yamana, K Shirouzu, K Itoh, A Yamada. 2000. CDw108 expression during T-cell development.. https://doi.org/10.1034/j.1399-0039.2000.550505.x

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