PubMed · 10539492
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M Theis. 1999. Temperature measuring devices.. https://pubmed.ncbi.nlm.nih.gov/10539492/
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Shiga toxin II (Stx2) has attracted extensive attention due to its toxicity and pathogenicity, making the development of sensitive detection methods urgent. This study constructed a dual-mode sensing platform for the sensitive detection of Stx2 in food. Composite material UIO-66@PdIr with peroxidase-like activity and fluorescent properties was synthesized and combined with cDNA as the signal probe, while aptamer-modified magnetic beads served as the capture probe. Specific binding of Stx2 to the aptamer triggered the release of the signal probe, enabling colorimetric and fluorescence signal readout. The colorimetric mode showed a linear range of 0.05-100 ng/mL with an LOD of 0.039 ng/mL, and the fluorescence mode exhibited 0.01-1000 ng/mL with an LOD of 0.0097 ng/mL. Additionally, this method was successfully applied to the detection of Stx2 in food, and the recovery rates were 94.33% ∼ 102.20%. It indicated that the constructed sensor holds great practical potential for Stx2 detection.
A method is described for determining 41 insecticide residues in garlic (Allium sativum L.), including organophosphorus, organochlorine, carbamate, and synthetic pyrethroid insecticides. These insecticides were extracted from samples with acetone and dichloromethane, and co-extractives removed using a charcoal/Celite/alumina column. Analysis was performed by gas chromatography with ion trap mass spectrometry in selective ion storage (SIS) mode. Retention times and specific ions (m/z values) were used to confirm insecticides. Recoveries for most insecticides (blank samples spiked at 0.05, 0.2 and 1 microg mL(-1) levels) ranged from 70% to 110%, the coefficient of variation (CV) of the method was <20% for every case, and the limit of detection (LOD), defined in terms of 3 times baseline noise, varied between 0.01 and 0.16 mg kg(-1), depending on the compound.
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