Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 10423556

Direct methods in electron crystallography: image processing and solving incommensurate structures.

H F Fan
View original source ↗Export metadata ↓

Abstract

Theory and practice of direct methods are given in detail for application in image processing of high-resolution electron microscopy and for solving incommensurate crystal structures. The effect of dynamical electron diffraction is also discussed.

Explore related subjects

CrystallizationCrystallographyImage Processing, Computer-AssistedIndolesMicroscopy, ElectronOrganometallic CompoundsProteinsJournal ArticleResearch Support, Non-U.S. Gov't

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

H F Fan. 1999-07-15. Direct methods in electron crystallography: image processing and solving incommensurate structures.. https://doi.org/10.1002/(sici)1097-0029(19990715)46%3A2%3C104%3A%3Aaid-jemt4%3E3.0.co%3B2-z

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Core-controlled polymorphism in virus-like particles.

This study concerns the self-assembly of virus-like particles (VLPs) composed of an icosahedral virus protein coat encapsulating a functionalized spherical nanoparticle core. The recent development of efficient methods for VLP self-assembly has opened the way to structural studies. Using electron microscopy with image reconstruction, the structures of several VLPs obtained from brome mosaic virus capsid proteins and gold nanoparticles were elucidated. Varying the gold core diameter provides control over the capsid structure. The number of subunits required for a complete capsid increases with the core diameter. The packaging efficiency is a function of the number of capsid protein subunits per gold nanoparticle. VLPs of varying diameters were found to resemble to three classes of viral particles found in cells (T=1, 2, and 3). As a consequence of their regularity, VLPs form three-dimensional crystals under the same conditions as the wild-type virus. The crystals represent a form of metallodielectric material that exhibits optical properties influenced by multipolar plasmonic coupling.

Crystallization↗