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PubMed · 10393297

Errors and reproducibility in electron-density map interpretation.

S L Mowbray·C Helgstrand·J A Sigrell·A D Cameron·T A Jones
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Abstract

Three investigators, with varying levels of experience, independently built and refined the structure of Escherichia coli ribokinase at 2.6 A resolution. At the end of the refinement/rebuilding processes the models had essentially converged, although each had its own particular pattern of remaining errors. The subsequent refinement of the same structure at 1.8 A resolution allowed an overall quality check of each of the lower resolution models, and an analysis of which graphics-based tools were generally most efficient in locating these errors. Criteria which are useful in the application of Ramachandran, main-chain and side-chain database and real-space fit analyses are presented.

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Crystallography, X-RayElectronsEscherichia coliPhosphotransferases (Alcohol Group Acceptor)Protein ConformationReproducibility of ResultsJournal Article

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S L Mowbray, C Helgstrand, J A Sigrell, A D Cameron, T A Jones. 1999. Errors and reproducibility in electron-density map interpretation.. https://doi.org/10.1107/s0907444999005211

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