PubMed · 10187052
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
K Southwick. 1998. Ready ... set ... wait..... https://pubmed.ncbi.nlm.nih.gov/10187052/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Explore the source record for details and available documents.
The incidence of post-transfusion hepatitis has been reduced greatly by screening blood donors for hepatitis B surface antigen (HBsAg). However, hepatitis B virus infection still accounts for a certain number of cases of post-transfusion hepatitis. The purpose of this study was to detect HBV DNA in the HBsAg negative blood samples by using nested PCR with two primer pairs specific to core region. Two hundreds blood samples from HBsAg negative donors, and 14 samples from HBsAg positive donors were provided by the blood bank of Ramathibodi Hospital. The results showed that HBV DNA was detected in all 14 HBsAg positive blood samples and in 7 (3.5%) of 200 HBsAg negative blood samples. This study showed that the absence of HBsAg in otherwise apparently healthy individuals may not be enough to ensure lack of circulating HBV. The more sensitive ELISA technique is still in need. Otherwise, the safety of blood transfusion can be enhanced by careful selection of blood donors and careful consideration of risks and benefit of the patients who need blood transfusions.
As of November 1997, a total of 2098 cord blood (CB) units are stored in the CB bank Düsseldorf (mean volume, total nucleated cell count, BFU-E, CFU-GM, CFU-GEMM, CD34+ cells). Considering a threshold of 2 x 10(7) nucleated cells (NC)/kg body weight required for transplantation and 10 +/- 5 x 10(8) NC per CB unit, 100%, 67% and 25% of the CB units contain sufficient NC to engraft patients of 10 kg, 35 kg and 50-70 kg, respectively. Within the last 3 years with the majority during 1997, a total of 4000 preliminary searches, 450 extended unit reports were submitted to the CB bank by fax or World Wide Web and 36 units were delivered upon request for transplantation to various centers. 1940 CB units were stored unseparated, frozen in two freezing bags requiring 15 400-l liquid nitrogen tanks for long-term storage. Therefore, the volume reduction method applying Hetastarch (HES) according to Rubinstein's protocol was introduced into the routine processing of the CB units. Until now 158 CB units with a mean recovery of 85 +/- 13% for nucleated cells, 86 +/- 12% and 84 +/- 13% for CFC and CD34+ cells, respectively, were processed and stored with a total volume of 25 ml in one freezing bag.