Linear SRY transcript in equine testis.

T Hasegawa; M Ishida; T Harigaya; F Sato; N Ishida; H Mukoyama

doi:10.1292/jvms.61.97

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Linear SRY transcript in equine testis.

T Hasegawa·M Ishida·T Harigaya·F Sato·N Ishida·H Mukoyama

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Abstract

Employing a combination of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques, the complete coding sequence of cDNA for the equine SRY gene was determined. We also attempted to make clear whether the equine SRY gene transcript is expressed in the adult testis, and whether the type of transcript is expressed as linear or circular RNA. As a result, in total a 1420 bp cDNA sequence was determined. Accomplishment of 3' RACE infers that equine SRY gene was expressed as a linear RNA transcript in testicular tissue just after puberty, in contrast to the situation in mice.

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Amino Acid Sequence Animals Cattle DNA, Complementary DNA-Binding Proteins Horses Humans Male Mice Molecular Sequence Data Nuclear Proteins Polymerase Chain Reaction Random Amplified Polymorphic DNA Technique Sex Determination Processes Sex-Determining Region Y Protein Testis Transcription Factors Transcription, Genetic Journal Article

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T Hasegawa, M Ishida, T Harigaya, F Sato, N Ishida, H Mukoyama. 1999. Linear SRY transcript in equine testis.. https://doi.org/10.1292/jvms.61.97

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