Biomedical subjects

Zhiming Yang

Publications and source records attributed to Zhiming Yang.

At least 37 records · Page 2Linked to original sources

PubMed2005

[Experimental study on repair of goat tibia defect with marrow stromal cell and bio-derived bone].

OBJECTIVE: To investigate the feasibility of repairing goat tibia defect with marrow stromal cells (MSCs). METHODS: MSCs were cocultured with the bio-derived bone in vitro, and the 20 mm tibia defects were made and fixed with plate in 35 goats, and they were divided into the experimental group, control group and blank group. The defects on the right side were filled with tissue engineering bone as the experimental group, the defects on the left side with bio-derived bone as the control group in 33 goats, and the defect on the both sides were not filled with any materials as the blank group in 2 goats. The repair capability was assessed physically, histopathologically and biomechanically at 2, 4, 6, 8, 12, 16 and 24 weeks after operation in 3 groups. RESULTS: By physical, histopathological and biomechanical examinations, the bio-derived bone was partially absorbed in the experimental group and was rarely absorbed in the control group in the 4th week; the defects were partially repaired in the experimental group, and in the control group, few new bones were observed in the two ends of the implants, in which there was fibrous tissue. The effects of biomechanics had no statistically significant difference between the experimental group and the control group (P>0.05) in the 8th week; the defects were perfectly repaired in the experimental group and the effects of biomechanics had statistically significant difference between two groups (P<0.05) in the 12th weeks. The defects were not repaired in the 24th week in the blank group. CONCLUSION: The tissue engineering bone can efficiently repair bone defect, and its repair capability is better than that of bio-derived bone alone both in quantity and in quality of bone formation.

Animals↗

PubMed2005

[Experimental study on repairing segmental bone defect with bio-derived bone preserved by various methods].

OBJECTIVE: To study the difference of repairing segmental bone defect with bio-derived bone preserved by various methods. METHODS: Freeze-dried biomaterials had been stored in two different preservation solutions for three months, while the biomaterials stored for same period were observed as control group. The experimental model of 15 mm radial segmental defect was made in 60 New Zealand white rabbits, which were divided into groups A, B and C according to transplant materials preserved by various methods. Groups A and B were deeply divided into A1 and A2 subgroups, B1 and B2 subgroups according to whether materials were cocultured with osteoblasts. Tissue engineered bone was used to repair bone defects of left limbs in A1 and B1 subgroups, while simple material to repair defects of right limbs in A2 and B2 subgroups. Group C was divided into C1 and C2 subgroups. Freeze-dried material was used to repair bone defects of the left limbs, while defects of the right limbs as blank control group. The samples were harvested and observed by the roentgenographical, histomorphological, biomechanical and computerized graphical analysis at 4, 8 and 16 weeks. RESULTS: All of the defects treated with implants exhibited new bone formation 4, 8 and 16 weeks postoperatively, increasing with time. The radiological, histomorphological and biomechanical evaluation showed that the ability of new bone formation was arranged in 6 subgroups as follows: A1>A2>C1>B1>B2>C2, the difference was significant between them (P<0.001, P<0.05). The ability of new bone formation was strongest and at 16 weeks the defect was bridged with the appearance of marrow cavities in A1 subgroup, the biomechanical properties in implants approached to those of normal bone. CONCLUSION: The choice of proper preservation solution can improve the ability of repairing bone defect.

Animals↗

PubMed2005

[Expression of interleukin 2 and IL-2 receptor after implanted tissue engineered bones constructed with allogeneic marrow stromal stem cells and bio-derived materials in rhesus monkeys].

OBJECTIVE: To explore the feasibility of allogeneic marrow stromal stem cells (MSCs) as seed cells to construct tissue engineered bone by detecting the expressions of interleukin 2 (IL-2) and IL-2 receptor in rhesus monkeys after implanting these tissue engineered bones. METHODS: Engineered bones were constructed with osteoblasts which derived from allogeneic MSCs and bio-derived materials in vitro, and then were implanted to bridge 2.5 cm segmental bone defects of left radius in 15 rhesus monkeys as experimental group, bio-derived materials only were implanted to bridge same size defects of right radius as control group. Every 3 monkeys were sacrificed in the 1st, the 2nd, the 3rd, the 6th and the 12th weeks postoperatively and the expressions of IL-2 and IL-2 receptor in blood and graft samples were detected quantitatively by enzyme-linked immunosorbent assay (ELISA). RESULTS: There was no significant difference in the contents of IL-2 and its receptor between 2 groups (P>0.05). The contents of IL-2 and its receptor increased from the 2nd week and maintained high level from the 2nd to the 6th week, but decreased after 6 weeks. CONCLUSION: Tissue engineered bones constructed with allogeneic MSCs and bio-derived materials show low immunogenicity. Allogeneic MSCs may be used as seed cells to construct tissue engineered bone.

Animals↗

PubMed2005

[Experimental study on biomechanics characteristics of combined collagen tissue engineering tendon].

OBJECTIVE: To investigate the influence of collagen on the biomechanics strength of tissue engineering tendon. METHODS: All of 75 nude mice were made the defect models of achilles tendons, and were divided into 5 groups randomly. Five different materials including human hair, carbon fibre (CF), polyglycolic acid (PGA), human hair and PGA, and CF and PGA with exogenous collagen were co-cultured with exogenous tendon cells to construct the tissue engineering tendons. These tendons were implanted to repair defect of achilles tendons of right hind limb in nude mice as experimental groups, while the materials without collagen were implanted to repair the contralateral achilles tendons as control groups. In the 2nd, 4th, 6th, 8th and 12th weeks after implantation, the biomechanical characteristics of the tissue engineering tendon was measured, meanwhile, the changes of the biomechanics strength were observed and compared. RESULTS: From the 2nd week to the 4th week after implantation, the experimental groups were stronger than the control groups in biomechanics, there was statistically significant difference (P < 0.05). From the 6th to 12th weeks, there was no statistically significant difference between the experiment and control groups (P > 0.05). Positive correlation existed between time and intensity, there was statistically significant difference (P < 0.05). The strength of materials was good in human hair, followed by CF, and PGA was poor. CONCLUSION: Exogenous collagen can enhance the mechanics strength of tissue engineering tendon, and is of a certain effect on affected limb

Animals↗

PubMed2005

[Experimental study on constructing muscle tissue in rabbits with tissue engineering methods].

OBJECTIVE: To explore the possibility of constructing tissue engineering muscles by combining allogeneic myoblasts with small intestinal submucosa (SIS) in rabbits. METHODS: A large number of purified myoblasts were obtained with multi-procedure digestion and repeated attachment method from skeletal muscles taken from extremities of immature rabbits which were born 7 days ago. The myoblasts were labeled with BrdU, and then combined with SIS to construct tissue engineering muscles. This kind of tissue engineering muscles were grafted into the gastrocnemius muscle defect (1.5 cm in length, 1.0 cm in width) of fifteen rabbits as the experimental group. The SIS was grafted into the same position in the control group. The rabbits were sacrificed 4, 6, 8 weeks after operation. The tissue engineering muscles were evaluated by macroscopic, histological and immunohistochemical observations, and by quantitative analysis of local immunocyte in the grafting site. RESULTS: Allogeneic myoblasts with SIS were combined perfectly in vitro. The SIS was connected tightly to surrounding skeletal muscles and inflammation response was obvious 4 weeks after grafting. The SIS began to break down and inflammation response became slight 6 and 8 weeks after operation. Compared with that of 8th week, the quantitative analysis of local immunocyte in 4th and 6th week in both experimental and control group has significance (P<0.05). Newly formed muscle tissues were found around SIS in the experimental group in 4th, 6th, and 8th week. Expression of BrdU and myosin immunohistochemical staining were positive in the experimental group and negative in the control group. CONCLUSION: Tissue engineering muscles of rabbits which are constructed by combining allogeneic myoblasts with SIS can survive and proliferate.

Animals↗

PubMed2005

[Experimental study of bone infection on WO-1 controlled-release system].

OBJECTIVE: To develop a new tissue engineering bone material which has an anti-infective function. METHODS: Collagen loaded bio-derived bone material was made by using type-I collagen and allograft bone. WO-1 was absorbed to collagen loaded bio-derived bone, then the morphological feature of the new bone material was observed by scanning electronic microscopy. 3H-tetracycline was diluted by WO-1 solution, and was absorbed to collagen loaded bio-derived bone, then the releasing kinetics of WO-1 was detected by 3H-tetracycline in vitro. WO-1 bio-derived bone material was grafted into a culture medium with staphylococcus aureus, Escherichia coli, and pseudomonas aeruginosa to observe its bacteriostasis ability. WO-1 bio-derived bone material was grafted into radius of defected rabbits, the concentration of WO-1 was detected on the 9th, 16th, 23th, and 30th day by HLPC in blood, in bone and in muscle. The bacteriostasis ability of WO-1 loaded bio-derived bone was tested in vitro and in vivo. RESULTS: WO-1 loaded bio-derived bone maintained natural network pore system and the surface of network pore system was coated with collagen membrane. The release of WO-1 from WO-1 loaded bio-derived bone showed bursting release on the 1st day, then showed stable release. WO-1 loaded bio-derived bone showed lasting and stable bacteriostasis to common pathogens of orthopaedic infections. The high concentration of WO-1 was observed in bone tissue and in muscle tissue at different time points and the difference among groups had no significance (P>0.05), while the concentration of WO-1 in blood was very low (P<0.05). CONCLUSION: WO-1 loaded bio-derived bone has good capability of drug controlled-release and bacteriostasis.

Animals↗

PubMed2005

[Morphological and biomechanical study on in vivo osteogenesis after repair of cranial defects with plastic engineered bone in rabbits].

OBJECTIVE: To investigate the morphology and biomechanics of in vivo osteogenesis after repairing rabbit skull defects with plastic engineered bone which was prefabricated with alginate gel, osteoblasts and bone granules. METHODS: Twenty-eight rabbits were divided into group A (n=16), group B (n=8) and group C (n=4). The bilateral skull defects of 1 cm in diameter were made. Left skull defects filled with alginate gel-osteoblasts-bone granules (group A1) and right skull defects filled with alginate gel-bone granules (group A2). The defects of group B was left, as blank control and group C had no defect as normal control. The morphological change and bone formation were observed by methods of gross, histology and biomechanics. RESULTS: In group A1, the skull defects were almost entirely repaired by hard tissue 12 weeks after operation. The alginate gel-osteoblasts-bone granule material had changed into bone tissue with few bone granules and some residuary alginate gel. The percentage of bone formation area was 40.92% +/- 19.36%. The maximum compression loading on repairing tissue of defects was 37.33 +/- 2.95 N/mm; the maximum strain was 1.05 +/- 0.20 mm; and loading/strain ratio was 35.82 +/- 6.48 N/mm. In group A2, the alginate and bone granules material partially changed into bone tissue 12 weeks after operation. The percentage of bone formation area was 18.51% +/- 6.01%. The maximum compression loading was 30.59 +/- 4.65 N; the maximum strain was 1.35 +/- 0.44 mm; and the loading/strain ratio was 24.95 +/- 12.40 N/mm. In group B, the skull defects were mainly repaired by membrane-like soft tissue with only few bone in marginal area; the percentage of bone formation area was 12.72% +/- 9.46%. The maximum compression loading was 29.5 +/- 2.05 N; the maximum strain was 1.57 +/- 0.31 mm; and the loading/strain ratio was 19.90 +/- 5.47 N/mm. In group C, the maximum compression loading was 41.55 +/- 2.52 N; the maximum strain was 0.95 +/- 0.17 mm; and the loading/strain ratio was 47.57 +/- 11.22 N/mm. CONCLUSION: The plastic engineered bone prefabricated with alginate gel-osteoblasts-bone granule may shape according to the bone defects and has good ability to form bone tissue, whose maximum compression loading can reach 89% of normal skull and the hardness at 12 weeks after operation is similar to that of normal skull.

Alginates↗

PubMed2005

[Experimental studies on preparation and property of scaffold material of bio-derived bone loading wo-1].

OBJECTIVE: To provide the chosen scaffold materials for experiment and application of tissue engineering and to detect the properties of the collagen bio-derived bone scaffold material loading WO-1. METHODS: The pure bio-derived bone scaffold material, bio-derived bone scaffold material loading collagen, collagen bio-derived bone scaffold material loading WO-1 were made by use of allograft bone, and type-I collagen, and WO-1. The morphological features, constitute components and mechanical properties were examined by scanning electron microscopy, X- rays diffraction and mechanical assay. RESULTS: The bio-derived bone scaffold material maintained natural network pore system; the bio-derived bone scaffold material loading collagen maintained natural network pore system, the surface of network pore system was coated by collagen membrane; the collagen bio-derived bone scaffold material loading WO-1 maintained natural network pore system, the surface of network pore system was coated by collagen membrane. The pore sizes of the 3 materials were 90-700 microm, 75-600 microm and 80-600 microm, respectively, and the porosities were 87.96%, 80.47%, 84.2%. There was no significant difference between them (P>0.05). The collagen bio-derived bone scaffold material loading WO-1 consisted of [HA,Ca10 (OH)2 (PO4)6. There was no significant difference in the mechanical strength of the three scaffold materials. CONCLUSION: The bio-derived bone scaffold material loading WO-1 is as good as bio-derived bone scaffold material and collagen bio-derived bone scaffold material, and it is an effective scaffold material for tissue engineering bone.

Biocompatible Materials↗

PubMed2005

[Effect of surface property of different polyether-ester copolymers on growth of smooth muscle cells and endothelial cells].

OBJECTIVE: To investigate the effect of surface property of different polyether-ester block copolymers [poly(ethylene glycol-terephthalate)/poly(butylene terephthalate), PEGT/PBT] on the growth of smooth muscle cells (SMCs) and endothelial cells(ECs). METHODS: Three kinds of copolymers were synthesized, which were 1000-T20 (group A), 1000PEGT70/PBT30 (group B) and 600PEGT70/PBT30 (group C). The water-uptake and contact angle of three polyether-ester membranes were determined. The canine aorta smooth muscle cells and external jugular vein endothelial cells were primarily harvested, subcultured, and then identified. The proliferation of SMCs and ECs on the different polyether-ester membranes were investigated. RESULTS: The water-uptake of three copolymers arranged as the sequence of group C group A> group B, indicating group B being more hydrophilic. However, smooth muscle cells and endothelial cells grew poorly on the membrane of group B after low density seeding, but proliferated well on the membranes of group A and group C. CONCLUSION: In contrast with more hydrophilic 1000PEGT70/PBT30, moderately hydrophilic 1000-T20 and 600PEGT70/PBT30 has better compatibility with vascular cells. The above results indicate that the vascular cells can grow well on moderately hydrophilic PEGT/PBT and that PEGT/PBT can be used in vascular tissue engineering.

Animals↗

PubMed2005

[The study of interaction between the osteoblast and bio-derived material by detecting the gene expression].

OBJECTIVE: To study the gene expressions of human osteoblasts during the construction of tissue engineered bone with the bio-derived material. METHODS: The fetal osteoblasts were used to construct tissue engineered bone with the bio-derived material and then were cultured 2, 4, 6, 8 and 10 days in vitro. Real-time PCR analysis indicated that Cbfa 1, Osterix, Collagen type I, osteocalcin (OC) and Integrin alpha5 and beta1 were present in osteoblasts with bio-derived materials. Results The change of Cbfa1 was consistent with the change of Osterix. On 2nd day and 8th day, the expression of Osterix in experimental group was higher than that in control group, P<0.05. Collagen type I's change was consistent with change of OC expression, and its expression was higher in experimental group than that in control group on 2nd, 4th, 6th and 8th day. The Integrin expression was high all along. Conclusion The important genes can be expressed normally by integrating osteoblasts with bio-derived scaffolds. As skeleton tissue engineering scaffold, the bio-derived bone is conducive to keep the osteoblast's phenotype and differentiation with osteo-conductive ability. The osteoblast can enter proliferation stage favorably and the scaffold materials exert no effects on it. Bio-derived bone can also supply more space for cells to proliferate. The bio-derived materials promote osteoblasts adhesion.

Adult↗

PubMed2005

[Experimental study of tissue engineered bone with cryopreservation on healing of bone defects].

OBJECTIVE: To investigate the effect of tissue engineered bone with cryopreservation on healing of bone defects and to explore feasibility of cryopreservation for tissue engineered bone. METHODS: Tissue engineered bones were constructed with osteoblasts being seeded onto bio-derived materials made from fresh human bones,and they were preserved at 4 degrees C and - 196 degrees C for 3 months and 6 months respectively. They were applied to repair segmental bone defects of rabbit's radius while the tissue engineered bone without cryopreservation and bio-derived materials were brought into control groups. The experiment was divided into groups A3, A6, B3, B6, C and D (group A3: tissue engineered bones were preserved at 4 degrees C for 3 months; group A6: tissue engineered bones were preserved at 4 degrees C for 6 months; group B3:tissue engineered bones were preserved at --196 degrees C for 3 months; group B6:tissue engineered bones were preserved at - 196 degrees C for 6 months; group C: tissue engineered bones without cryopreservation; group D: bio-derived materials). Macroscopical and histological examination were done at the 2nd, 4th, 6th, 12th weeks, X-ray examination was done at the 6th, 12th weeks and biomechanics were determined at 12th weeks after operation respectively. RESULTS: Macroscopical observation showed no significant differences among group A3, A6, B3, B6 and C, but less new bone formation and more obvious boundary in group D were observed. Histological observation showed more collagen and new bone around the edge of implant of group A3, A6, B3, B6 and C than group D, and histological evaluation showed significant differences between group D and other groups (P <0.05). Radiographic observation showed no absorbability of the implant cortex and less new bone formation in group D, but the unity between implant and host bone, medullary cavity reopened, disappearance of fracture line and fine bone modelling were observed in other groups at 12 weeks after operation. Biomechanics between group D and other groups showed significant differences (P< 0. 05). CONCLUSION: Cryopreservation (4 degrees C and - 196 degrees C) were capable of preserving tissue engineered bone for long time, and tissue engineered bone with cryopreservation has significant effect on healing of bone defects. The methods fit clinical application.

Animals↗

PubMed2005

[Elementary evaluation of small intestinal submucosa and polypropylene mesh used for repairing abdominal wall defect in rats].

OBJECTIVE: To compare the effect of small intestinal submucosa (SIS) and polypropylene mesh (PPM) on repairing abdominal wall defects in rats, and to probe into the feasibility of using SIS to repair the abdominal wall defects. METHODS: 100 SD rats (50 males and 50 females)were randomly divided into 2 groups (n = 50). Their weight ranged from 200 to 250 g. Full thickness abdominal wall defects (2 cmX 2 cm) were created by surgery and were repaired with SIS and PPM respectively. At different postoperative time (1st, 2nd, 4th, 8th and 12th week), animals were sacrificed to make histological observation. The tensile strength and the development of adhesions were measured and observed. RESULTS: 95 animals survived and were healthy after surgery. No inflammatory response and obvious immunoreaction were observed in both groups. One week after operation, the tensile strength of abdominal wall in SIS group (204.30+/-5.13 mmHg) was lower than that in PPM group(240.0+/-10.0 mmHg) at 1st week (P<0.05), and there were no difference at 4th, 8th, 12th week. Adhesions were more marked in PPM group than that in SIS group (P<0.05). CONCLUSION: Both SIS and PPM are histologically compatible when used in rats and can maintain sufficient tensile strength. SIS is superior to PPM in regards to tissue compatibility and adhesion formation.

Abdominal Injuries↗

PubMed2005

[Therapeutic effect of tissue engineered tendon in repairing old calcaneal tendon rupture and defects].

OBJECTIVE: To probe into the surgical methods and therapeutic effect of repairing old calcaneal tendon rupture and defects with tissue engineered tendons. METHODS: The tissue engineered tendons were prefabricated by co-cultivating allogeneic tendon cells with composite of carbon fiber and polyglycolic acid for 5 days. From August 1999 to June 2002, 7 patients with calcaneal tendon rupture and defects (5-7 cm in length) were treated with tissue engineered tendons. The defects were repaired by suturing repeatedly with tissue engineered tendons. Meanwhile, the defects were covered by gastrocnemius fascial flap for protection and strengthening. After surgery, the ankle joints were fixed with plaster 4 to 6 weeks, and then the functional exercise was done. RESULTS: All the patients were followed up 22 to 56 months (46.9 months on average). Six patients achieved healing by the first intention, only one patient had delayed union. No local or systemic complication occurred in all the cases. No patients were given the second operation for adhesion. In accordance with YIN Qingshui's criterion for therapeutic effect, the results were excellent in 5 cases, good in 1 case and moderate in 1 case. CONCLUSION: Repairing old rupture and defects of calcaneal tendon with tissue engineered tendons can achieve good clinical outcome, it is an optional therapy.

Achilles Tendon↗

PubMed2005

[Biological effect of WO-1 on human embryonic osteoblasts].

OBJECTIVE: To investigate the effect of WO-1 on the proliferation and differentiation of human embryonic osteoblasts (HEO) and to provide research methods of bone tissue engineering. Methods HEO were isolated from periosteum and calvaria and then cultured in vitro. The dose-effect relationship between WO-1 concentration and biological effect of HEO was evaluated by growth curve and 3H-TdR count. The effect of WO-1 on cell activity and proliferation was investigated by cloning efficiency, cell cycle analysis was determined by flow cytometer and morphological was examined through transmission electron microscope. Moreover, the effect of WO-1 on osteoblastic function was evaluated at protein and mRNA levels by ALP activity, 3H-proline incorporation, osteocalcin secretion (RIA) and mRNA expression of type I collagen and osteocalcin (RT-PCR). Results The proliferation of HEO was inhibited in high concentration of WO-1, while it was promoted in low concentration of WO-1. The optimal dose was 8 microg/ml, and there was dose-effect relationship in the certain range of WO-1 concentration (0.25 microg/ml to 8 microg/ml). In 8 microg/ml of WO-1, the cloning efficiency and cloning volume of HEO were increased, population doubling time was decreased. All indexes of osteoblastic function including ALP activity, type I collagen synthesis and osteocalcin secretion were increased, the more sufficed cell organs were observed under transmission electron microscope than control group (P < 0.05). CONCLUSION: WO-1 can promote the cell activity and proliferation of HEO cultured in vitro in low concentration, enhance the synthesis of extracellular matrix, such as type I collagen and osteocalcin, and accelerate the mineralization of osteoid. WO-1 can be used as a stimulant of proliferation and differentiation of HEO in the research of bone tissue engineering, which provide the theoretical basis in clinical application.

Bone Substitutes↗

PubMed2005

[Progress in vitreous cryopreservation of living bioproducts].

In this brief review, some key issues related to vitreous cryopreservation of living tissues (natural or engineered), including cells, embryos, tissues, organs, and engineered tissues, are outlined. The principle of vitreous cryopreservation for the biological activity and functionality is demonstrated. The procedures of cooling/ rewarming, composition and function of optimal cryoprotectants, and their effects on bioproducts are described. Vitrification could, therefore, prove to be a useful and effective method of bioproduct cryopreservation for a long period of time, particularly for organized tissues and organs. However, the toxicity of the cryoprotective agents and the devitrification occurring during the rewarming process need additional investigations. Several key areas of research on vitrification are also addressed.

Cryopreservation↗

PubMed2005

[Functional reconstruction of irreversible partial injury of brachial plexus].

OBJECTIVE: To assess treating results of functional reconstruction of irreversible partial injury of brachial plexus and to improve the function of injured upper extremity. METHODS: Seventy-nine cases with irreversible partial injury of brachial plexus were treated in transfer of muscle (tendon) or by functional arthrodesis (fixation of tendon) from January 1984 to June 2003. According to the evaluation criterion by American Shoulder and Elbow, Hand Association, all patients were followed up in motion of reconstructive joint and daily activities after operation for 1 year to 19 years. The effect of the operation was comprehensively scored and evaluated. RESULTS: Final results in 54 cases were as follows: 30 patients with good results, 19 patients with fair results, and 5 with poor results. The results demonstrated some points as follow: (1) if the shoulder was instable, arthrodesis of shoulder would be a better choice; (2) the flexion of the elbow joint should be only reconstructed with the dynamic reconstructive methods. The reconstruction of flexion of elbow by transfer of pectoral major muscle was more effective than that by transfer of flexor carpi ulnaris muscle; (3) the dynamic reconstruction of extension of digital and carpi was better than that of flexion of digital and opposition function of the thumb; (4) the supination of the forearm was effectively reconstructed by transfer of flexor carpi ulnaris muscle. Pronation teres muscle should be studied more in reconstruction of supination function of the forearm. CONCLUSION: The functional reconstruction is effective in treating irreversible partial injury of brachial plexus.

Adolescent↗

PubMed2005

[The application of basic fibroblast growth factor in tendon tissue engineering].

OBJECTIVE: To review the recent researches of basic fibroblast growth factor (bFGF) in tendon tissue engineering. METHODS: Recent original related literature was extensively reviewed and analyzed. RESULTS: bFGF played an important role in establishing standard tendon tissue engineering cell lines, inducing the compound and analysis of extracellular matrix, enhancing interactions between cells and extracellular matrix and accelerating tissue engineering materials' neovascularization. CONCLUSION: The progresses in increasing endogenetic bFGF expression, controlling the release of exogenous bFGF and improving the bio-utilization of bFGF has laid foundation for wider use of bFGF in tendon tissue engineering.

Animals↗

PubMed2005

[Appropriate application of statistical analysis method in medical science and technology articles].

OBJECTIVE: To introduce the appropriate application of statistical analysis method in medical science and technology articles. METHODS: Explained the correct application of statistical theories in statistical package, statistical analysis methods and test criterion which compose the basic statistical content in an article. RESULTS: If the distribution of numerical variable is normal, mean and standard deviance can be used to describe this variable. In the same way, t test and analysis of variance (ANOVA) can be used to test the difference of mean in each group. If it is not normal, median and range can be used to describe the variable and rank sum test can be used to test the difference of distribution in each group. Categorical variable can be described by rate, proportion and ratio. There are chi-square test, fisher's exact test and rank sum test to test the difference of rates. CONCLUSION: It is the key of choosing rational statistical methods to distinguish the type of design and variable.

Analysis of Variance↗