Biomedical subjects

Z Zhou

Publications and source records attributed to Z Zhou.

At least 343 records · Page 19Linked to original sources

PubMed1997

[Study on the effect of ofloxacin and tanshinone II A on human leukocyte chemotactic migration in vitro].

In order to verify the possible anti-inflammatory activity and their potency of ofloxacin and Tanshinone II a, the effects of different concentrations of ofloxacin, Tanshinone II a and hydrocortisone on human leukocyte migration in vitro were investigated employing agarose migration technique in which f-Met-Lea-Phe was served as chemotactic factor. The results showed that all the three agents could inhibit migration of leukocytes remarkably. The effects were concentration-dependent with excellent linear relationship. The IC50 were: Tanshinone II a 0.46 microgram/ml, ofloxacin 0.63 microgram/ml, and hydrocortisone 46.52 micrograms/ml respectively. The results indicated that Tanshinone II a was the most potent agent for inhibiting migration of human leukocytes in vitro and the hydrocortisone was relatively less potent.

Abietanes↗

PubMed1997

[Fully length MDR1 cDNA transfer conferring resistance to adriamycine on sensitive cells GLC].

Human lung cancer leads the mortality of cancers and the chemotherapy is often uneffective because of drug resistance. In order to study the role of mdr-1 gene in resistant lung cancer, the fully length mdr-1 cDNA was transferred into a sensitive lung cancer cell line GLC. The mdr-1 cDNA was constructed in a retroviral vector, pDORneo. The transfection of recombinant plasmid was carried out by lipofectin. Supernatant containing infective viruses derived from a G418 resistant clone of package cell PA317 was used to infect GLC cell which is sensitive to chemotherapeutic agents. After G418 and adriamycine selections, three P-glycoprotein positive clones were isolated and the integration of mdr-1 cDNA was demonstrated by PCR of genomic DNA. The relative resistance of 3 clones to adriamycine as and elevated by 5.4, 6.0 respectively 7.8 times compared with the untransfected cell and the transcription of mdr-1 gene in these transfected cells as obviously enhanced by in situ hybridization. This results suggest that the mdr-1 gene plays a role in increasing drug resistance of human lung cancer.

Adenocarcinoma↗

PubMed1997

[Ameloblastic carcinoma and malignant amelobastoma: histological reunderstanding].

Twenty-four cases of malignant ameloblastoma were reported, among them were nineteen cases followed up for 5 to 30 years. Histologic characteristics include predominated proliferation of the peripheral cells with papillary protruding to stroma, replacement of the stellate reticular cells in the follicular center by sarcomatous cells, as well as large and deep-stained or alveolar nuclei with moderate atypia, and high mitosis of the tumor cells. Clinico-radiologically, the tumor showed malignant features such as fast growing recently, pain, anesthesia, cauliflower ulcer, limited mouth opening, and large multilocular radiolucent area with ill-defined border. The biological behavior, nomenclature of the ameloblastic carcinoma or malignant ameloblastoma, and differential diagnosis from other tumors were also discussed.

Adolescent↗

PubMed1997

[Molecular cloning of the imipenem resistant gene in bacteroides fragilis].

Imipenem, as a representative of-cabapenem, is one of the most effective beta-Lactam agents against Bacteroides, but recently there have been a few reports on the resistant strains of Bacteroides. To explore the mechanism of imipenem resistance (IMPr) at the molecular level, we extracted chromosome DNA of the resistant strains and acquired a 1.7 kb fragment of resistant gene of imipenem. Recombinant plasmid was stably reserved in generation after generation in E. coli DH5. Resistant gene of imipenem was labeled with Dig-11-dUTP by way of random prime, and 40 strains of clinically isolated Bacteroides were detected by the prepared DNA probe. Two IMPr strains showed hybridization signal, whereas 38 IMPr strains did not show such a signal. The result suggests that the Dig-labeled IMPr gene probe can be used in molecular epidemiology for investigating IMPr strains.

Bacteroides fragilis↗

PubMed1997

[Medicine S inhibits class II MHC expression of intragraft renal in rats].

The Purpose of this study was to address the mechanism of medicine S which has anti-rejection effects of renal allografts in rats. Kidney transplantations were performed from SD to Wistar strain (allogeneic) and from Wistar to Wistar (Isograft) using the same modified technigue described by Fabre and kamada. Experimental rats were divided into five groups. Group I (Isograft group) and group II (allograft group) as controls were not treated with medicine. The others were allograft groups which received medicine S, Cyclosporine A, and low-dose Cyclosporine combined with medicine S, respectively. Renal function and resultant morphology changes were assessed 2, 4 weeks after transplantation. All sections of kidney grafts were stained with monoclonal antibody class II MHC (OX6), and then the surface densities of positive staining were quantified by computer image analysis. The level of molecular expression in group II was significantly increased (7.61 +/- 0.57 vs 0.51 +/- 0.2 of group I, P < 0.01). In groups I and IV, the molecule of expression was reduced, compared with the groups II, III and V (P < 0.05). The results suggest that medicine S decreases the level of class II MHC expression and medicines combined with lowe-dose cyclosporine is more effective than cyclosporine alone.

Animals↗

PubMed1997

[Diagnosis and treatment of primary malignant tumors of the small bowel].

OBJECTIVE: To study the clinical characteristics, effective diagnostic methods and therapeutic measures of primary malignant tumors of the small bowel so as to improve therapeutic effect. METHODS: In this paper 75 cases of primary malignant tumors of the small bowel were analyzed. Survival rates were calculated with Life Table, and computer Cox multivariate analysis model was used for survival analysis. RESULTS: The most frequent clinical manifestations of primary malignant tumors of the small bowel were abdominal pain, abdominal mass, emaciation and intestinal obstruction. Gastrointestinal barium meal roentgenography was performed in 13 cases, with an accurate diagnostic rate of 84.6%. The 1-,3- and 5-year survival rate after radical resection was 87.5%, 68.7% and 48.1%, respectively, while that for palliative resection was 57.9%, 33.8% and 24.1%, respectively. The factors including age, histological type, tumor site and type of resection were selected into Cox model in survival analysis. CONCLUSION: For primary malignant tumors of the small bowel, gastrointestinal barium meal roentgenography is the most valuable method for diagnosis, and surgical radical resection is the most effective therapy. The significant prognostic factors are age, histological type, tumor site and type of resection. Chemotherapy had no effect on prognosis.

Adenocarcinoma↗

PubMed1997

[The study on analgesic effect of local-release delivery system--Huangjiabang].

Laboratory investigation was made on analgesic effect of local release delivery system, Huangjiabang, which mainly includes Chinese medical herbs. Through the application of twisting-body and thermal-plate experiments on white rats. The result showed that the pain threshold was markedly elevated (P < 0.05, P < 0.001) after the rats were fed with Huangjiabang 125 mg/kg or 250 mg/kg body weight, and the number of twisting body's animals significantly decreased (P < 0.001). The analgestic mechanism of Huangjiabang delivery system relates to its effect of invigorating the circulation of blood and accelerating the stasis assimilation, remitting the inflammation.

Analgesics, Non-Narcotic↗

PubMed1997

[mRNA expression of metastasis-suppressor gene nm23 in carcinoma of buccal mucosa I. Northern blotting study].

In this study the authors detected both nm23-H1 and nm23-H2 mRNA levels in 52 tissues samples of patients with carcinoma of buccal mucosa (CBM) by Northern blotting method. The expression levels of both nm23-H1 and nm23-H2 mRNA in CBM were higher than those in normal buccal mucosa, leukoplakia, adjacent nontumorous mucosa, and lymph nodes with or without metastasis. The nm23-H1 mRNA expression levels in CBM with lymph nodes metastases were lower than those in CBM without metastasis, and were lower in metastatic lympho nodes (P < 0.05). Of the 11 cases with metastases, 9 cases (81.8%) showed low expression of nm23-H1 mRNA, of 19 cases without metastasis, 15 cases (78.9%) were high expression. No significance of nm23-H2 mRNA expression levels were found between CBM with and CBM without metastasis (P > 0.05). The expression of nm23-H1 and nm23-H2 mRNA showed no significant correlation with each clinical-pathological parameter examed. The result suggested that the expression of nm23-H1 mRNA significantly correlated inversely with lymph node metastasis in CBM, while the expression of nm23-H2 mRNA not. nm23 mRNA expression levels can provide a valuable predictor of lymph node metastases in CBM.

Antigens, Neoplasm↗

PubMed1997

[Experimental study on increasing arterial oxygen saturation by healthy exerciser under hypoxia].

The study was carried out among 41 healthy subjects born in Shanghai (Sea level). They were divided into two groups. Group one (10 subjects) was studied at sea level in a hypobaric chamber (3000m). Group two (31 subjects) was studied in high altitude plateau (Xigatse at 3836m). Arterial oxygen saturation (SaO2), Heart Rate (HR), blood pressure (BP) and myocardial oxygen uptake (MVO2) were measured noninvasively with Nelleor N-200 pulse Oxymeter and HEM-812F-Digital Automatic Blood Pressure Monitor. SaO2 increased significantly (from 87.7 +/- 1.7% to 95.3 +/- 1.0% P<0.01 and from 86.4 +/-1.0% to 91.8 +/- 0.6%, P < 0.05, respectively) by healthy exerciser through 15 min in both groups. After 3 min exercise SaO2 was still significantly higher than before exercise. Then HR, BP and MVO2 have not change significantly. The study results suggested that this kind of exerciser may help increasing the SaO2 and thus could be used to prevent and treat acute hypoxic response to some extent.

Altitude↗

PubMed1997

[Quantitative method of toluol diffusive sampling].

The diffusive sampling is of great advantage to air pollutant monitoring in manned spaceflight cabin. The quantitative method and steps of diffusive sampling is given in this paper by an experimental studying on diffusive sampling of toluol-air mixture; Effectiveness of the method and effects of environmental factors on the quantification of the diffusive sampling were discussed. This method is of reference value to quantification of the polycomponent-air mixture.

Air↗

PubMed1997

[Vertical transmission of hepatitis C virus].

To study the transmission of hepatitis C virus (HCV) from infected mother to their infants, out of 1047 mothers studied, 8 were anti-HCV antibody and 6 were HCV RNA positive as well as 2 mothers who were anti-HCV antibody and HCV RNA positive. 12 mothers and their 12 babies were followed up for 12 months. Only one of 12 infants showed persistently positive for anti-HCV antibody and HCV RNA at 1, 6 and 12 months of age. His HCV type was identical to his mother. These results suggest that vertical transmission is present but not an important route for HCV infection.

Adult↗

PubMed1997

[Brush-type chiral stationary phase for chiral resolution in high performance liquid chromatography].

A review is presented about brush-type chiral stationary phase in high performance liquid chromatography chiral resolution, dealing with design, synthesis and applications, of various brush-type chiral stationary phases (CSP). The advances of study of chiral recognition mechanism are introduced and the development trends of the brush-type chiral stationary phase in the future proposed. Eighty-four references are cited in this paper.

English Abstract↗

PubMed1997

[Enrichment and determination of trace chlorobenzene compounds in water].

In this work, by using GDX-402 porous polymer beads as adsorbent and carbon disulfide as eluent, the conditions for enriching trace chlorobenzene compounds in water were investigated. Enriched eluate was directly injected into a column packed with 3.0% dihexyl sulfonic acid sodium/101 white support to be separated, and then detected by flame ionization detector under constant temperature. The method has been used successfully for the determination of trace of chlorobenzene compounds in water.

Chlorobenzenes↗

PubMed1997

[Effect of nickel sulfate on the concentrations of T3, T4 and TSH in serum of rat].

The effect of nickel sulfate on the concentrations of T3, T4 and TSH in sera of rats was studied. Forty wistar male rats were divided into four groups. The rats in groups 1, 2 and 3 were injected with 0.005 mol/L NiSO4, 0.01 mol/L NiSO4 and 0.02 mol/L NiSO4 [1 ml/(kg x d)] respectively, while the rats of group 4 were injected with normal saline, serving as control. Forty days later, the concentrations of T3 and T4 in sera of 0.01 mol/L NiSO4 and 0.02 mol/L NiSO4 groups were obviously decreased (P<0.05 or P<0.01). The concentrations of T4 in sera of 0.005 mol/L NiSO4 group was also decreased, compared with control group. There was no difference in the concentrations of T3 and T4 in sera among the groups with different doses of NiSO4. Between different dose groups and the control group, the concentrations of TSH in sera showed no significant difference (P>0.05). The proliferation of the epithelial cells of thyroid gland was noticed. The rough endoplasmic reticulam was exceedingly expanded. The nuclei were transformed. The results suggest that Ni may injure thyroid gland.

Animals↗

PubMed1997

[Determination of Ni, Al, Mn and Si in copper based alloy by high current and microsecond pulsed glow discharge optical emission spectrometry].

The determination of Ni, Al, Mn and Si in copper based alloy by high current and microsecond pulsed (HCMSP) glow discharge optical emission spectrometry (GD-OES) has been reported. With the laboratory built experiment set-up, the factors effecting analytical performance have been studied. The results of sample analysis are in good agreements with the recommendation values, and the stansard deviation for six determinations were 2.66% for Ni, 2.17% for Mn, 6.02% for Si, 1.00% for Al respectively.

English Abstract↗

PubMed1997

A difference in nuclear morphometry when frozen rather than fresh tissue is studied.

OBJECTIVE: Since the value of nuclear morphometry as a prognostic indicator in gynecologic malignancies is beginning to be explored, the authors attempted to see whether there was a difference in the nuclear morphometric features of size, shape and summed optical density in endometrial cancer when touch preparations were made from fresh versus frozen tissue. METHODS: Fifty specimens were obtained from twenty-five consecutive patients with endometrial cancer. Each patient had an air dried touch preparation made from fresh and frozen tissue. Values were compared using paired samples T-test. Logistic regression was used as a type of multivariate analysis. Nuclear size (NUSZ), shape (NUSH), and summed optical density (NUSD) were calculated by image analysis. RESULTS: Mean NUSZ was shown to be significantly larger in fresh specimens (117.3 microns2) when compared to the frozen specimens (89.3 microns2) p < 0.001. Mean NUSH was significantly closed to round in frozen specimens (15.21) when compared to the fresh specimens (15.55) p = 0.038. Mean NSUD was shown to be significantly larger in frozen specimens (128.6) as compared to fresh specimens (120.2) p = 0.045. Stage, NUSZ was NUSD from fresh tissue were shown to be independent predictors of recurrent disease. CONCLUSION: The type of tissue (fresh versus frozen) from which touch preparations are made is important. Significant differences in NUSZ, NUSH, and NUSD were seen between fresh and frozen specimens. While NUSZ and NUSD from fresh specimens were predictive of recurrence, the same parameters from frozen specimens were not. A larger prospective trial is underway to determine the validity of these preliminary conclusions.

Cell Nucleus↗

PubMed1997

[Research respects and status of functional neuromuscular stimulation].

Functional Neuromuscular Stimulation (FNS) is a technique of restoring movements of paralyzed patients. This paper describes several respects of functional neuromuscular stimulation, such as stimulators, sensors, electrodes, muscle-skeletal models and control methods. Some novel control strategies are also discussed in this paper.

Electric Stimulation Therapy↗

PubMed1997

[Evaluation of optimal measuring site and index by QDR4500A for postmenopausal bone loss].

As the function of most bone mineral measuring machine is limited, either by single photon absorptiometry (SPA), dual photon absorptiometry (DPA), speed of sound (SOS), or dual energy x-ray absorptiometry (DEXA), it is hard to evaluate the best place and index of postmenopausal bone loss at many positions by one machine at the same time. So we used QDR4500A, one of the best advanced DEXA which has a good repeatability, to search the best place and index for postmenopausal bone loss. The results showed that (1) bone mineral density (BMD) was better than bone mineral content (BMC) and Area to reflect this kind of bone loss; (2) lumber BMD was a better place than the forearm BMD, total spine BMD and other places; and (3) midlateral L3, 4 BMD was a best index for postmenopausal bone loss.

Absorptiometry, Photon↗