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Biomedical subjects

Z Zhou

Publications and source records attributed to Z Zhou.

At least 235 records · Page 13Linked to original sources
PubMed1999

[Effects of laminin on the invasive and metastatic behaviors of human salivary adenoid cystic carcinoma cell lines].

OBJECTIVE: To study the effect of laminin (LN) on invasive and metastastic behaviors of salivary adenoid cystic carcinoma (Acc) cell lines. METHODS: Immunohistochemistry, Boyden chamber in vitro motility, and adhesion test were used. LN and LNR (LN receptor) distributions in Acc-2 and Acc-M cells were studied with immunohistochemical staining. RESULTS: There were less LN and LNR found in Acc-2, but much more LN and LNR were present in the highly metastatic clone Acc-M. With the presence of exgenous LN, Acc-2 became activated showing a higher migration rate. The cell adhesion test showed that Acc-2 had stronger ability of adhesion than Acc-M with the presence of LN. CONCLUSION: The result suggests LN and LNR may play a vitral role in the invasion and metastasis of Acc.

Animals↗
PubMed1999

[Vascularized illiac bone grafts and reconstruction of the mandible].

OBJECTIVE: To investigate method of reconstructing large mandible defects. METHODS: Seven cases of mandibular defects were reconstructed with vascularied illiac bone grafts supplied by circumflex illiac vessels and Bränemark osseointegration implants immediately. RESULTS: Six months after operation, X-rays showed osseointegration between implants and graft bone. After 6 months to 36 months of follow-up, all implants were stable and no radiolucency was found and the functions were satisfactory in mastication, pronunciation and facial contour. CONCLUSION: Reconstruction of large mandible defects by vascularized illiac bone grafts supplied by circumflex illiac vessels and Bränemark osseointegration implants immediately is promising.

Adolescent↗
PubMed1999

[Clinical and experimental study on effect of Shuguan granule on mid-late rheumatoid arthritis].

OBJECTIVE: To explore the therapeutic mechanism of Shuguan Granule (SGG) in treating mid-late rheumatoid arthritis. METHODS: Based on the principle of reinforcing Kidney to treat arthritis, removing phlegm to remove stasis, two SGG, Shuguan Wenjing granule (SW) and Shuguan Qingluo granule (SQ) were prepared and used to treat mid-late 44 and 43 patients of rheumatoid arthritis respectively. The clinical result was compared with that of Wangbi Granule. Animal experiments on the effect of the two SGG were conducted. RESULTS: The total effective rate of the SW, SQ and control group was 88.64%, 93.02% and 73.17% respectively. Results of experimental studies in rats with adjuvant-induced arthritis showed that the two SGG obviously raise the level of serum SOD, and lowered the levels of serum interleukin-1, plasma prostaglandin E2 and thromboxane B2, as compared with the model group, the difference was significant (P < 0.05, P < 0.01). CONCLUSION: The two SGG have a comprehensive function of anti-inflammatory, analgesia, antioxidation, antihypercoagulation and immunoregulation.

Adult↗
PubMed1999

[The feature of pancreatic microcirculatory impairment in caerulein induced acute pancreatitis].

OBJECTIVE: To study the feature of pancreatic microcirculatory impairment in caerulein-induced acute pancreatitis. METHODS: The AP model of subcutaneous injection of caerulein was studied by intravital fluorescence microscopy of erythrocytes labeled by FITC (FITC-RBC) and by scanning electron microscopy of vascular corrosion casts and light microscopy of Chinese ink-injected/cleared tissues. RESULTS: Animals treated with caerulein showed hyper-amylases. Contractions of intralobular arteriolar sphincter, presence of vacuoles in all the layers of sphincter, gross irregularity in capillary network of acini were observed in caerulein-induced groups. The decrease of pancreatic capillary blood flow (P < 0.01), reduction of functional capillary density, and irregular intermittent perfusion (P < 0.05) were found. CONCLUSIONS: Impairment and contraction of pancreatic intralobular arteriolar sphincter are the initial microcirculatory lesions in the early phase of acute pancreatitis induced by Caerulein, and play a key role in the pancreatic ischemia and pancreatic microvascular failure in acute pancreatitis.

Acute Disease↗
PubMed1999

[Effects of occupational exposure to formamidines on cardiovascular functions].

OBJECTIVE: To understand the possible effects of occupational exposure to formamidines on human cardiovascular function, this study was carried out. METHODS: Sixteen farmers spraying chlordimeform, 14 packers packaging chlordimeform and 23 packers packaging mono-formamidine were followed-up pre- and post-exposure. Their urinary excretion of formamidine or its metabolite, as well as air concentrations of formamidine at their work places and their skin contamination with it were measured to estimate the exposure level. Furthermore, 24-hour urinary level of vanillinmandelic acid (VMA) was analyzed among mono-formamidine packers. RESULTS: Their whole body skin contaminated with 3.240 and 2.142 g/cm(2) of chlordimeform in the sprayers and packers, respectively. Their hand skin contaminated with mono-formamidine at 6.59 g/cm(2) in the packers. It indicated that the major route of exposure to formamidine was skin contamination. Urinary levels of formamidines or their metabolites increased significantly after exposure, reaching 6.194 and 3.378 micromol/L for the sprayers and packers exposed to chlordimeform, respectively, and 2.760 - 3.427 micromol/L for mono-formamidine in the packers. Their heart rates slowed down, P-R and Q-T intervals prolonged and blood pressure reduced after exposure, as compared with those before exposure. Consistency of the results in several studies demonstrated that formamidines could induce changes in the indices for cardiovascular functions under the relatively low exposure levels at present. Decrease of urinary VMA from 0.068 micromol/L pre-exposure to 0.040 - 0.055 micromol/L post-exposure suggested that catecholamine could play a role in these effects. CONCLUSION: Formamidines has certain effects on human cardiovascular function. It is suggested that attention be paid to the changes in cardiovascular functions of those exposed in their health surveillance.

Adult↗
PubMed1999

[Studies on DNA single strand breaks induced by sodium nitroprusside-nitric oxide donor].

OBJECTIVE: To study the effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on DNA single strand breaks (SSBs). METHODS: A modified method was used to isolate and prepare the single cell suspension from the organs of mice. Alkaline single-cell gel electrophoresis (SCGE) was performed to examine DNA damage of the cells treated by SNP in vivo and in vitro. RESULTS: Treatment with 0.5 - 2.0 micromol/ml of SNP with S(9) for 1 h induced a concentration-dependent increase in DNA SSBs in g12 cells. Significant increase in DNA migration and comet frequency in the spleen, thymus and peritoneal macrophage were induced after intraperitoneal injection of SNP at a dose of 0.67 - 6.0 mg/kg. No obvious increase in DNA single strand breaks was observed in the liver, kidney and lung of the mice with same treatment. CONCLUSION: DNA SSBs could be induced by NO in some cells in vivo and in vitro. There was difference in sensitivity of various organs in the mice to NO. Cells of spleen and thymus and macrophage may be the important target cells of NO.

Animals↗
PubMed1999

[Study on the antidiuretic efficacy and mechanism of indapamide in central diabetes insipidus].

OBJECTIVE: To investigate the efficacy and antidiuretic mechanism of indapamide in central diabetes insipidus. METHODS: We observed eight patients with central diabetes insipidus treated by indapamide (2.5-7.5 mg.d-1) or dihydro-chlorothiazide (50-70 mg.d-1) or carbamazepine (150-300 mg.d-1) for 8 days with randomized block design. The therapy was changed twice in sequence after 4 days without treatment. Urine output and indexes of water metabolism were also observed. RESULTS: The urine output was reduced on the 4th day and 8th day with each therapy. The serum potassium and chloride concentration was decreased and renin activity was increased on the 4th and 8th day with indapamide therapy. CONCLUSION: Indapamide has antidiuretic action on central diabetes insipidus. The action may result from the changes of hydroelectrolytes and renin-angiotensin-aldosterone system.

Adolescent↗
PubMed1999

[Clinical analysis of 17 patients with adrenal "incidentaloma"].

This paper reports 17 cases of adrenal "incidentaloma", discovered by B-mode ultrasonography, computed tomography and/or magnetic resonance imaging scan and identified by operation and pathological examination. Seven of them were pathologically diagnosed as adrenocortical adenoma, 4 pheochromocytoma, 2 adrenal cyst, and 1 mixed cell adenoma, 1 leiomyoma, 1 adrenocortical carcinoma, and 1 gangliocytoma. Adrenal shows hormone-secreting function in 17 patients with adrenal "incidentaloma". The diagnosis and management of this disorder are discussed.

Adenoma↗
PubMed1999

[Study on the prevention of insulitis and diabetes by complete Freund's adjuvant in NOD female mice].

The degrees of insulitis, the incidence of diabetes, and the counts of alpha,beta and delta cells in islets were studied by morphological method and avidin-biotin complex immunohistochemical method in complete Freund's adjuvant(CFA) or normal saline(NS)-treated non-obese diabetic(NOD) female mice. The results showed: The incidences of in-insulitis and the scoring means were significantly lower in CFA-treated mice than those in NS-treated ones; none of 5 CFA-treated mice and 3 out of 5 NS-treated ones developed diabetes. The positive rates of alpha, and delta cells were significantly lower in CFA-treated mice than those in NS-treated ones, but the positive rate of beta cell was significantly higher in CFA-treated mice than that in NS-treated ones. The correlations were found among the scores of insulitis and the positive rates of alpha, beta, delta cells in islets (r alpha = 0.475, r beta = -0.878, r delta = 0.869). The results indicate that CFA may lessen the degrees of insulitis and the incidence of diabetes. Its effects might be related to lessen the damage of beta cell in islets.

Animals↗
PubMed1999

[Change of high density lipoprotein receptor of hepatocyte during cholesterol gallstone formation in rabbit model].

In order to study the formation of cholesterol gallstone through rabbit model which was induced by high cholesterol diet (HCD), we investigated the high density lipoprotein receptor (HDLr) activities of hepatocytes, total bile acids and cholesterol of common duct bile at 1 week(1 w), 2 weeks(2 w), 3 weeks (3 w) and 4 weeks(4 w) in comparison with those of a control group respectively. The results were as follows: 1. The HDLr Bmax decreased and the kd value increased significantly (1 w group vs control group, P < 0.05); In 2 w group the two variables recovered to the level of control group; and after that, the HDLr Bmax increased and Kd value decreased markedly (3 w and 4 w groups vs control group, P < 0.05). 2. Total bile acids in bile increased slightly in 1 w group (vs control group, P > 0.05), then decreased in 2 w, 3 w and 4 w groups (4 w group vs control group, P < 0.05). 3. The concentration of cholesterol in bile increased gradually as the time of feeding HCD went on (3 w and 4 w groups vs control group, P < 0.05). The results demonstrated that owing to the intake of HCD with the passage of time, the activity of HDLr was up at the beginning and down gradually in the rest period. The change in concentrations of bile acids was similar to that of HDLr, and the concentration of cholesterol in bile was foreign to the change of HDLr. These results suggest that the decrease of HDLr activity may cause the absence of material for bile acids synthesis and it may play an important role in the formation of gallstone.

Animals↗
PubMed1999

[Ameloblastic fibrosarcoma: an immunohistochemical and ultrastructural study of the mesenchymal component].

To study the characteristics of the mesenchymal cells of ameloblastic fibrosarcoma (AFS), three cases of AFS were studied immunohistochemically and ultrastructurally. The results showed that the mesenchymal component of AFS consisted predominantly of fibroblastic cells with a small number of undifferentiated cells, a few histiocytes and occasionally myofibroblastic cells under electron microscope. The fibroblastic cells were Vimentin positive only, and myofibroblastic cells were positive for Vimentin, HHF35 and alpha-SMA. The histiocytes were positive both for kp1 and PG-M1, suggesting that these cells were infiltrating cells from peripheral blood rather than histiocytic differentiation of tumor cells. Compared with ameloblastic fibroma, AFS showed much higher PCNA labeling index, suggesting higher proliferation activity of AFS.

Fibrosarcoma↗
PubMed1999

[Assay of aluminum content in alum and alum-processed medicinal herbs and its distribution in blood and brain in mice].

OBJECTIVE: To assay the aluminum content in alum and some Chinese medicinal herbs processed with alum, and compare the aluminum distribution of these herbs in blood and brain in mice. METHOD: Aluminum content of the herbs was assayed with spectrophotometry, snd aluminum distribution in blood and brain was assayed with atomic absorption spectrophotometry. RESULTS: Aluminum content in alum was found higher than that in the alum-processed herbs. Aluminum distribution in blood and brain after oral administration at equimolecular doses, equivalent to 5.7 mg/kg of metal Al, was studied in mice, and no significant differences were observed. CONCLUSION: Aluminum distribution in blood and brain in mice is related to the dosages of aluminum administered and has little to do with the kind of herbs used. All these medicinal herbs do not improve the absorption of aluminum in mice.

Alum Compounds↗
PubMed1999

[Chiral separation of thio-glycidyl ether on amylose-chiral stationary phase by high performance liquid chromatography].

In the present study, we synthesized amylose-tris(phenylcarbamate), and used it as chiral stationary phase based on silica gel. This stationary phase offered a practically useful chiral separation medium. Amylose was allowed to react with an excess of phenylisocyanate in pyridine for 48 h. The obtained carbamate was dissolved in DMF and adsorbed on silica gel previously treated with 3-aminopropyltriethoxysilane. The mass ratio of the carbamate to the silica gel was 0.45/2.55. The four thio-glycidyl ethers were resolved on the amylose tris(phenylcarbamate) chiral stationary phase. Separation was carried out with a hexane/2-propanol mixture as mobile phase at a flow rate of 1.0 mL/min at room temperature. The effects of 2-propanol concentration on the retention and resolution have been investigated.

Amylose↗
PubMed1999

[Neural network approach to medical grading of rheumatoid arthritis].

A grading system based on neural network has been developed and used int he medical grading diagnosis of a case group of Rheumatoid Arthritis presenting Cold syndrome intermingled with Heat syndrome. The possibility of using neural network in TCM diagnosis is preliminarily explored in this paper.

Algorithms↗
PubMed1999

[Chiral separation of thioglycidyl ether and glycidylselenide on cyclodextrin bonded stationary phase via high performance liquid chromatography].

beta- Cyclodextrin bonded chiral stationary phases (CD-CSP) were prepared after the method of Armstrong (CD-CSP I) and of Huang Tianbao (CD-CSP II). With the separation of o-, m- p-nitroaniline, it was found that the chromatographic properties of CD-CSP II was superior to that of CD-CSP I. Three racemes of thioglycidyl ethers and two racemes of glycidylselenides were resolved on CD-CSP II via high performance liquid chromatography. The mobile phase consisted of different volume ratios of methanol/0.3% triethylammonium acetate buffer solution(pH 5), and the eluate was detected with a UV-visible detector at a wavelength of 254 nm. With the increase of methanol volume ratio in the mobile phase, the retention time decreased rapidly, but within the range of methanol valume ratio 40/100 to 50/100, the separation factor only decreased slightly. The racemes examined contained aromatic groups and hydroxy groups which can form inclusion complexes and hydrogen bonding interactions with beta-cyclodextrin. The chiral separation of the racemes is successful with the mobile phase of V(methanol):V(buffer) = 40:60.

Aniline Compounds↗
PubMed1999

[Clinic observation on the prothetic results of dental supply implant system].

OBJECTIVE: To make a clinical evaluation by the Dental Supply implant system by three years follow-up study. METHODS: 46 patients treated by Dental Supply implant system were chosen for three years follow-up investigations. RESULTS: The ball-bar framework complete denture was the best and the prostheses for single molars were almost a failure, as the success rate was only 14.3%. CONCLUSIONS: Dental Supply system was not appropriate to restore the single tooth and posterial fixed bridge.

Adult↗