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Biomedical subjects

Z Z Wang

Publications and source records attributed to Z Z Wang.

At least 19 recordsLinked to original sources
PubMed2007

Treatment of acute promyelocytic leukemia by retinoids.

We review the role of all-trans retinoic acid (ATRA) in the treatment of acute promyelocytic leukemia (APL). The combination of ATRA and conventional anthracycline-ARA-C chemotherapy (CT) has clearly demonstrated its superiority over CT alone (in terms of relapse and survival) in newly diagnosed APL. Combination treatment probably also reduces the incidence of initial failures, and complete remission (CR) rates greater than 90% are now regularly reported in large multicenter trials. Some randomized studies strongly suggest that prolonged maintenance treatment (for 1 or 2 years) with ATRA and low-dose CT, and possibly very early introduction of anthracycline CT during induction treatment, may reduce the incidence of relapse. With those treatments, the relapse risk appears to be only 10%-15%, although it remains greater in patients who initially have high white blood cell counts (often associated with variant M3 morphology, short bcr3 isoform, etc.) and patients with residual disease detectable by RT-PCR at the end of consolidation courses. In those patients, addition of arsenic derivatives to induction or consolidation treatment (or both treatments together) may prove useful and is currently being tested. ATRA syndrome (now generally called APL differentiation syndrome, as it is also seen with arsenic derivatives) remains the major side effect of ATRA treatment. It occurs in 10%-15% of patients and is currently fatal in at least 10% of them. Rapid onset of CT or high dose steroids (or both) should improve its outcome. A sizeable proportion of APL patients who relapse after ATRA and CT can be durably salvaged by the same treatment followed by allogeneic or autologous stem cell transplantation, provided the transplant (in the autologous setting) is RT-PCR-negative. However, in relapsing APL arsenic derivatives (mainly arsenic trioxide) are now considered to be the reference treatment. Some of the current issues with ATRA treatment in newly diagnosed APL include whether ATRA has a role during consolidation treatment and whether arabinoside (AraC) is required in addition to anthracyclines in the chemotherapy combined to ATRA.

Antineoplastic Agents↗
PubMed2002

[Nphe1]nociceptin(1-13)-NH2 antagonizes nociceptin-induced hypotension, bradycardia, and hindquarters vasodilation in the anesthetized rat.

The purpose of this study was to investigate the effects of [Nphe1]nociceptin(1-13)-NH2 on nociceptin-induced decreases in mean arterial pressure (MAP), heart rate (HR), and hindquarters vascular bed resistance (HVBR) of the anesthetized rat. The results showed that i.c.v. or i.v. [Nphe1]nociceptin(1-13)-NH2 (1.5-12 nmol/kg and 5-120 nmol/kg, respectively) could antagonize the depressor effects of i.c.v. or i.v. nociceptin (3 and 30 nmol/kg, respectively) on MAP and HR. Furthermore, [Nphe1]nociceptin(1-13)-NH2 (5-120 nmol/kg) could reverse nociceptin (30 nmol/kg)-induced decrease of HVBR. However, [Nphe1]nociceptin(1-13)-NH2 had no significant effects on similar effects induced by morphine. Our results suggest that [Nphe1]nociceptin(1-13)-NH2 acts as a selective antagonist of the nociceptin receptor in the cardiovascular system of the rat.

Anesthesia↗
PubMed2001

[Effects of different methods of fetal spinal cord tissue transplanted on reversing the axotomy-induced neurons atrophy rats injured spinal cord].

OBJECTIVE: To explore the effects of different methods of fetal spinal cord(FSC) tissue transplanted on reversing the axotomy-induced neurons atrophy of adult rats injured spinal cord. METHODS: One hundred and twenty adult rats received lumbar spinal cord hemisection. Experimental rats were divided into five groups, the control group(Group A); spinal cord hemisection only(Group B); spinal cord hemisection plus FSC transplant (Group C); spinal cord hemisection plus FSC transplant plus pedicled paraspinal muscle(Group D); spinal cord hemisection plus FSC transplant plus pedicled omentum (Group E). Combined behavioral scores(CBS), somatosensory evoked potentials (SEP), motor evoked potentials(MEP) were examined to evaluate the recovery of neurological function after operation. Rats were sacrificed after 1, 4 and 12 weeks. Nissl stained section was used for neurons quantitative image analysis. The positive cells were quantitative analysis by computer image analysis system. RESULTS: The different methods of FSC tissue transplantation could prevent the neurons atrophy secondary to axon injury of spinal cord in adult rats. The size of neurons were observed in five groups, they were group E > group D > group C > group B > group A (P < 0.05). Those increases in size of neurons were paralleled with a significant improvement in neurological function recovery. CONCLUSION: It indicates that the different methods of FSC tissue transplantation can maintain the neurons morphology and improve the neurological function of rats.

Animals↗
PubMed2001

[Clone and expression of human soluble CD14 and study of its function].

Human soluble CD14(sCD14) cDNA fragment was amplified using total RNA extracted from U937 cells by RT-PCR of sCD14 gene, and the recombinant expression plasmid pEF1/HisC/sCD14 348aa was constructed. Then the expression in eukaryotic cell was carry out by liposome transfection method. It demonstrated that the expression level was relatively high by scanning map identification. The expressed product was purified by immunoaffinity chromatography and the purity was above 90%. The changes of CD14 brought by LPS stimulating U937 cell proved the product had the function of combine with LPS.

Cloning, Molecular↗
PubMed2001

[Triterpenoid saponins from Eclipta prostrata L].

AIM: To study the triterpenoid saponins in the Chinese traditional medicine Eclipta prostrata L.. METHODS: Column chromatography with silica gel and HPLC were employed for the isolation and purification. The molecular structures were determined on the basis of spectral analysis (IR, MS, 1HNMR, 13CNMR, HMQC and HMBC). RESULTS: Two new triterpenoid saponins, named eclalbasaponins XI (4) and XII (5), were obtained and their structures were elucidated as 3-O-[beta-D-glucopyranosyl(1-->2)-beta-D-glucopyranosyl]-16 alpha-ethoxy-olean-12-ene-28-oic acid-28-O-beta-D-glucopyranoside and 3-O-[(2-O-sulfuryl-beta-D-glucopyranosyl) (1-->2)-beta-D-glucopyranosyl]-echinocystic acid-28-O-beta-D-glucopyranoside, respectively, along with three known saponins, eclalbasaponins II (1), I (2) and III (3). CONCLUSION: Compounds 4 and 5 are new compounds, 1 and 5 induced morphological deformation of Pyricularia oryzae mycelia.

Antifungal Agents↗
PubMed2001

[The study advances and prospects of processing surface EMG signal in prosthesis control].

The surface EMG signal has direct relations with extremity movements and has become the ideal control signal for man-machine bionic system. This paper discusses the analytic study of surface EMG signals and gives various character estimations. Two kinds of character recognition methods of surface EMG are introduced for controlling prosthesis movements. The existing problems of multi-function EMG prosthesis control are summed up. In the end prospects for the study on analytic ways of surface EMG signal and the practical applications are discussed too.

Algorithms↗
PubMed2001

[6A8 alpha-mannosidase catalyzes p-nitrophenyl-alpha-D-mannopyranoside].

OBJECTIVE: To confirm the alpha-mannosidase nature of the protein encoded by 6A8 cDNA. METHODS: 1) To construct a full-length 6A8 cDNA based on the three cloned DNA fragments by means of gene recombinant technique; 2) To insert the 6A8 cDNA into eukaryotic expression vector pCDI; 3) To transfect the recombinant pCDI-6A8 into COS-7 cells; 4) To characterize the nature of the protein encoded by 6A8 cDNA by means of enzymic activity assay and Western blotting assay. RESULTS: The constructed 6A8 cDNA was the right cDNA in sequence. The enzymetic activity of the homogenate of COS-7 cells transfected with pCDI-6A8 was 3-4 times higher than that of the cells transfected with the mock or the wild cells. The enzymetic reaction could not be inhibited by swainsonine. Western blot showed a band of 120,000 recognized by mAb 6A8. The band in the cells transfected with pCDI-6A8 cDNA was much darker than that in the cells transfected with the mock or in the wild cells. CONCLUSION: The protein encoded by 6A8 cDNA is a kind of alpha-mannosidase, which belongs to type II alpha-mannosidase.

Animals↗
PubMed2001

[An approach for proper recording of the unit discharge in auditory nerve and inferior colliculus].

OBJECTIVE: To explore a proper recording of the unit discharge in auditory nerve and inferior colliculus for the observation of characteristics of response potential. METHODS: Single unit discharge in auditory nerve and inferior colliculus of healthy adult cat to acoustic stimulation and intracochlear electrical stimulation were recorded. With glass microelectrode. RESULTS: (1) the spontaneous discharge in auditory nerve primary fibers were identified. (2) The waveform of the spontaneous discharge in auditory nerve primary fibers was biphasic potential with positive pulse width 0.5 ms. (3) The discharge rate of auditory nerve ranged from several PPS to 100PPS. (4) The response in inferior colliculus to high frequency pure tone stimulation lacked phase locking. (5) The response in inferior colliculus to low frequency electrical stimulation in cochlea showed phase locking. CONCLUSIONS: It was successful to record the spontaneous discharges and auditory evoked responses in auditory nerve as well as the pure tone evoked response and electrically evoked response in inferior colliculus. However, the existing technical problem in recording the electrically evoked response in auditory nerve was to keep the response potential stable.

Acoustic Stimulation↗
PubMed2000

Cloning, expression and characterization of a cDNA (6A8) encoding a novel human alpha-mannosidase.

A 3300-bp cDNA (6A8) has been isolated from a human tonsil cell lambdagt11 cDNA library (GenBank accession number: AF044414). The 6A8 gene is localized on human chromosome 13q31-32. Its cDNA has an open reading frame from position 57 bp to 3243 bp, encoding a 1062 amino-acid polypeptide. The sequence of the polypeptide has 89% identity to rat liver ER alpha-mannosidase. Homogenates of COS-7 cells transfected with 6A8 cDNA showed an enhanced enzymatic activity with p-nitro-phenyl-alpha-D-mannopyranoside, which was not inhibited by swainsonine. These data suggest that 6A8 alpha-mannosidase belongs to the class II alpha-mannosidase. Western blot analysis showed a band for 6A8 cDNA encoded protein of approximately 120 kDa. Northern blot analysis revealed two 6A8 mRNA transcripts with different tissue distribution. Enhanced concanavalin A (ConA) binding to CNE-2L2 cells transfected with a reverse 6A8 DNA was observed, indicating that the 6A8 protein is an important cellular alpha-mannosidase.

Amino Acid Sequence↗
PubMed2000

The prevalence of NIDDM and IGT and related factors among residents in some areas of Hubei Province, China.

The epidemiological survey of prevalence of NIDDM (non-insulin dependent diabetes mellitus) and IGT(impaired glucose tolerance) was conducted among 9450 residents aged 25-70 in some areas of Hubei Province, China. The results show that NIDDM and IGT prevalences are 2.62% and 4.48%, respectively. There is no significant difference between male and female (P > 0.05). The NIDDM prevalence in cities is slightly higher than that in countryside, but the difference is not significant (P > 0.05). However, the IGT prevalence in city is significantly higher than that in countryside (P < 0.01). The prevalence of both NIDDM and IGT is increasing along with the age of the population. It is also significantly related to the family history of NIDDM, hypertension, and high body mass index (BMI). By using stepwise logistic regression to analyse the risk factors of NIDDM, age (OR = 1.86), BMI(OR = 2.69), family history (OR = 2.84) and hypertension (OR = 2.23) entered the model (significance level is alpha = 0.05).

Adult↗
PubMed2000

Plasma CPU-86017 concentrations regarding suppression of ouabain-induced cardiac arrhythmias and decrease of heart rate in guinea pigs.

AIM: To determine the effective plasma levels of CPU-86017 which could suppress the cardiac arrhythmias induced by i.v. ouabain in guinea pigs. METHODS: The cardiac arrhythmias and the heart rate were monitored by ECG traces. Blood samples were collected to determine plasma levels using HPLC assay. TXB2 and 6-keto-PGF1 alpha were measured in plasma. RESULTS: The plasma concentrations of CPU-86017 which were effective to suppress ventricular fibrillation (VF) and heart rate were 0.13-0.23 mg/L and 0.13-0.31 mg/L, respectively. A reduction of TXB2 levels and an elevation of 6-keto-PGF1 alpha levels were observed after CPU-86017 i.v. administration. CONCLUSION: The arrhythmia-suppressing and heart rate-slowing effect of CPU-86017 followed a linear relationship with its concentrations in plasma.

6-Ketoprostaglandin F1 alpha↗
PubMed2000

[Complexity analysis of surface EMG signals].

OBJECTIVE: Since the neurophysiological system is a highly nonlinear dynamic system, nonlinear dynamic information of EMG signals were extracted to describe its characteristics. METHOD: Two-channel surface EMG signals were extracted and analyzed to reflect the complexity degree of the dynamics of the neurophysiological system. RESULT: Complexity measures of four kinds of forearm motions were calculated and compared. They showed a good separability. CONCLUSION: Experimental results proved that this measure, having a simple algorithm, is suitable for short data sets and suitable for real time processing. It provides a new measurable index for both physiological and pathological analysis.

Electromyography↗
PubMed2000

[Identification of turtle shell, tortoise plastron and their counterfeit products].

OBJECTIVE: To identify the commercial Chinese medicines turtle shell and tortoise plastron. METHOD: Morphological identification and differential thermal analysis (DTA). RESULT: 2 kinds of counterfeit turtle shell and 3 kinds of counterfeit tortoise plastron were found, and their morphological and DTA identification features were proposed. CONCLUSION: Sea turtle shells presently on the market are sham commodities of turtle shell, which derive from Lissemys punctata and Pelochelys bibroni indigenous to south Asia, while plastrons of Ocadia sinensis, Chelonia mydas and Lepidochelys olivacea are counterfeit products of tortoise plastron. Turtle shells, tortoise plastrons and their counterfeit products may be exactly distinguished by morphological and DTA features.

Animals↗
PubMed2000

[A new telemedicine system].

A new telemedicine system and its application are introduced in this paper. Many new technologies, such as multiteleconference, multibind of MODEM and ISP, are adopted, which greatly improve the performance of the system.

Computer Communication Networks↗
PubMed2000

[AM signals' digital demodulation and its application in physiological signals' remote transmission].

Taking advantage of the characteristics of digital systems, a new digital method is given in this article to overcome the difficulties in demodulating AM signals using digital methods. Physiological signals can't be transmitted through telephone networks because they are full of ultra-low frequency components. However, if they are modulated (such as AM), they can be transmitted to the receiving end through telephone networks. Then phaselocked loops can be employed to trace the frequency of the signals' carriers so that we can demodulate and transmit them in the end.

Algorithms↗
PubMed2000

[A surface EMG signal identification method based on short-time Fourier transform].

A surface EMG signal identification method based on short-time fourier transform is presented in this paper. To fully utilize the nonstationary character of the EMG signal, short-time fourier transform is employed to get the signal's time-frequency representation. Singular value decomposition is then used in the spectrogram to extract feature vector for pattern identification. Four types of movement of forearm, hand grasp, hand extension, wrist pronation and wrist supination can be identified from surface EMG signals. Experimental results show that it is a stable and efficient method for extracting features.

Electromyography↗
PubMed1999

Metabolic stabilization of muscle nicotinic acetylcholine receptor by rapsyn.

Although the metabolic half-life of muscle endplate acetylcholine receptor (AChR) changes during development and after denervation in the adult, little is known about the molecular mechanisms that influence receptor stability. We have investigated the effect on AChR turnover of its interaction with rapsyn, a 43 kDa peripheral membrane protein that is closely associated with the AChR in muscle cells and is required for its clustering at endplates. Both in transfected COS cells and in cultured myotubes from rapsyn-negative and rapsyn-positive mice, we have found that the presence of rapsyn slows the turnover of AChRs by as much as twofold. The effect was similar for both embryonic (alpha2betadeltagamma) and adult (alpha2betadeltaepsilon) AChRs and for AChRs whose beta subunit lacked a putative tyrosine phosphorylation site. Neither colchicine nor cytochalasin D altered AChR turnover or prevented the rapsyn effect. Mutant rapsyn proteins whose N-terminal myristoylation signal was eliminated, or whose C terminus or zinc-finger domains were deleted, failed to change the rate of receptor turnover. Each of these mutations affects the association of the AChR with rapsyn, suggesting that AChR stability is altered by interaction between the two proteins. Our results suggest that, in addition to its role in AChR clustering, rapsyn also functions to metabolically stabilize the AChR.

Amino Acid Sequence↗