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Biomedical subjects

Z Yu

Publications and source records attributed to Z Yu.

At least 145 records · Page 8Linked to original sources
PubMed1999

Sweat gland adenoma with predominant myoepithelial differentiated features: case report and immunohistochemical study.

A 56-year-old man presented with a subcutaneous mass adjacent to the sternoclavicular joint region, which had predominant spindle-shaped myoepithelial cells and a small number of tubular or trabecular epithelial cells that resembled eccrine differentiation under a light microscope. Immunohistochemical studies showed positive staining for muscle-specific actin and vimentin in the myoepithelial cells, but staining was negative for S-100 protein and keratin. The tubular and trabecular epithelial cells stained positively for keratin, EMA and CEA. We believe that this rare tumor had the potential for biphasic differentiation and should be differentiated from other biphasic differentiated or mixed tumors in this site. Immunohistochemical staining was useful for diagnosis and differential diagnosis.

Adenoma↗
PubMed1999

[Genetic complementation group analysis of xeroderma pigmentosum patients in China].

OBJECTIVE: To establish skin fibroblast cell strains derived from Chinese xeroderma pigmentosum(XP) patients and to analyze the genetic complementation group and distribution. METHODS: From four XP patients, skin fibroblast cell strains were derived and used for the complementation group analysis by autoradiography and cell-fusion technique. RESULTS: Of the four XP patients from apparently independent families, three were assigned to group C, and one belonged to group E. This is the first report on XP-E patient in China. CONCLUSION: Based on the data from the previous 7 cases and the present 4 cases, the distribution of complementation group in Chinese XP patients known at present is: nine XP-C, one XP-F and one XP-E. Although the number of cases is still limited, XP-C appears to be more frequent in China. This forms a marked contrast to Japanese XP patients, who are dominated by XP-A with infrequent presence of XP-C.

Adolescent↗
PubMed1999

Upper GI bleeding.

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Acute Disease↗
PubMed1999

Upper GI bleeding

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Journal Article↗
PubMed1999

Effects of total parenteral nutrition on rat bile canaliculi: a stereologic analysis.

OBJECTIVE: To quantitate, in a stereologic manner, changes in bile canalicular morphology before and after choleretic infusion of total parenteral nutrition (TPN) and to determine whether TPN produces changes in localized regions within the hepatic lobule. STUDY DESIGN: Livers were obtained from sham-operated on normal adult male rats (control) and from rats that received intravenous TPN solution containing 20% glucose and 3.5% Molipron F. The tissues, obtained by a rigorous sampling procedure, were systematically subjected to stereologic analysis. Measurements were made on electron micrographs at two levels of magnification by point, intersection and profile counts, and then volume, surface area and length were estimated per unit parenchymal volume. RESULTS: The surface area of the canalicular wall per parenchymal volume increased significantly (from 5.33 x 10(-2) to 6.73 x 10(-2) microns 2/micron 3) after TPN treatment, as did the length of microvilli (from 0.241 to 0.267 microns/micron 3). However, the volume of bile canalicular lumina per parenchymal volume (0.306% and 0.320%), total length of bile canaliculi (1.05 x 10(-2) and 1.06 x 10(-2) microns/micron 3) and diameter of microvilli (8.73 x 10(-2) and 8.94 x 10(-2) microns) remained constant. CONCLUSION: These results indicate that changes in canalicular shape and microvillus hypertrophy may cause lowering efficiency of the bile flow rate.

Animals↗
PubMed1999

[Induction and validation of arterial calcium overload in rats].

OBJECTIVE: To induce arterial calcium overload (ACO) in rats and analyze its features. METHODS: Arterial calcium content and intracellular calcium content were determined by atomic absorption spectrometer and electron probe microanalysis, respectively. The morphological changes of endothelial cells and vascular smooth muscle cells were studied with scanning and transmission electron microscopes. DNA contents and its mass distribution were determined by DNA imaging analysis. Arterial collagen and elastin contents were determined biochemically. RESULTS: Seven days after the induction of ACO with selected regimen, massive calcium was found accumulating in the arterial wall, including the cytoplasm and mitochondrion of the medial smooth muscle cells, indicating both extracellular and intracellular calcium overload. Degeneration of aortic endothelial cells was found at the early stage of ACO under scanning and transmission electron microscope. At the late time of ACO, significant proliferation and sclerotic reactions of arteries occurred, including the proliferation of smooth muscle cells, matrix remodeling, and damage of elastic structure. The average DNA content per smooth muscle cell was elevated, and more cells entered the S phase of proliferating cycle since day 7. On the other hand, total collagen content of aorta was increased 7 days after induction of ACO, and rose continuously at day 14 (P < 0.05). Functional studies showed that arterial elasticity was significantly impaired. CONCLUSION: Our method could induce ACO/constantly in rats, and the ACO model shares the similarities with the characterization of human arteriosclerosis on many aspects.

Animals↗
PubMed1999

Expression of leukocyte adhesion molecules CD11b, L-selectin and CD45 during hemodialysis.

OBJECTIVE: To examine the gene and protein expression of CD11b, L-selectin and CD45, and the relationship between their expression and leukocytopenia during a hemodialysis session. METHODS: Ten maintenance hemodialysis patients, 20 uremic non-dialysis patients and 10 healthy volunteers were included in this study. The mRNA expression of CD11b, L-selectin and CD45 in peripheral blood mononuclear cells was detected by RT-PCR, while cell surface expression of these molecules on monocytes was detected by flow cytometry and leukocyte number was counted manually. RESULTS: After the start of dialysis, both mRNA expression and cell surface expression of CD11b increased rapidly, while those of L-selectin and leukocyte number fell. The mRNA expression of CD45 first decreased and the cell surface expression increased, followed by a return to pre-dialysis levels, by the end of dialysis. CONCLUSIONS: Hemodialysis using a cuprophane membrane can induce rapid upregulation of CD11b and down-regulation of L-selectin, which might influence the normal adhesion and anti-inflammatory response of leukocytes. In this process CD45 may play a role in regulating and/or transducing activation signal.

Adult↗
PubMed1999

Subclavian steal syndrome: report of 28 cases.

OBJECTIVE: To study the experience in the treatment of subclavian steal syndrome (SSS) and the relation between SSS and the damage of brain. METHODS: In 28 patients with SSS, 23 were operated on. They complained of vertigo, TIA, and claudication of arms. One patient suffered from severe stem ischemia spasm. All the patients were subjected to subtraction angiography. Fifteen of them showed retrograde flow in the vertebral arteries. Transcranial Doppler revealed retrograde blood flow in 8 patients. Single photon emission tomography showed inadequate blood supply to the brain with affected subclavian arteries in 4 patients. Carotid-subclavian arterial bypass was performed in 16 patients, axilloauxillary bypass in 3, PTA in 1, and aorta-carotid graft bypass in 3. RESULTS: 13 patients (13/23) had their cerebrovascular symptoms relieved. Transcranial Doppler and emission tomography after operation showed nothing abnormal. CONCLUSION: SSS is harmful and the patients should be closely monitored by means of transcranial Doppler and emission tomography.

Adult↗
PubMed1999

[Radiotherapy for early anal cancer: a report of 27 cases].

OBJECTIVE: To evaluate the results of radiotherapy for early stage anal cancer. METHODS: During 1960 through 1993, 27 patients with stage T1-2N0 anal cancer were treated by radiotherapy alone. Seven cases received pelvic irradiation plus local field boost, 20 cases received local field irradiation alone. Kaplan-Meier method was used in survival analysis. RESULTS: The overall 5-year survival rate in this series of patients was 79.1%. Eighteen of the 27 cases had their anal functions preserved. Local recurrence occurred in 7 cases and regional lymph node metastasis in one case. Five of them were salvaged, two by surgery and three by irradiation. CONCLUSION: Radiotherapy alone for early anal cancer is effective. Anal function is preserved in about two-thirds of the patients so treated. Local treatment failure can be salvaged by surgery or radiotherapy.

Adult↗
PubMed1999

[Clinical study on tangshen mixture in treating diabetic nephropathy].

OBJECTIVE: To investigate the effects of Tangshen Mixture (TSM) on the serum glucose, quantity of proteinuria per 24 h, hypercoagulative status and oxidative injury in patients with diabetic nephropathy (DN). METHODS: Ninety-three patients with DN were randomly divided into treated group and control group. 48 patients in the former were treated with insulin and TSM, while the latter was treated with insulin alone. Before and after treatment, the levels of fasting blood glucose (FBG), 2 h after meal blood glucose (PBG), 24 h urinary albumin (24 h-pro), endothelin (ET), alpha-granule membrane glycoprotein-140 (GMP-140), erythrocyte superoxide dismutase (ESOD), lipid peroxidase (LPO) were measured. RESULTS: After two months treatment, compared with the control group the levels of ET and GMP-140, LPO, and 24 h-pro lowered obviously (P < 0.01, P < 0.05), and the level of ESOD increased (P < 0.05) in the treated group, no obvious change was found in control grout after treatment. CONCLUSION: TSM could improve the hypercoagulative status, alleviate the renal free radical disturbances, and reduce the urinary protein loss.

Adult↗
PubMed1999

[Effect of plasminogen activator inhibitor 1 gene transfer into mesangial cells on Extracellular matrix accumulation].

OBJECTIVE: To observe the influence of plasminogen activator inhibitor 1 (PAI-1) overexpression on extracellular matrix (ECM) accumulation of rat mesangial cells. METHODS: PAI-1/GFP green fluorescent protein fusion gene eukaryotic expression vector pCMX-PAI-1-GFP was constructed and delivered into cultured rat mesangial cells by liposome. PAI-1/GFP expression was observed by fluorescent microscope. FN, LN and Type IV collagen levels were measured by Northern blot analysis and ELISA method. RESULTS: Northern blot analysis, SDS-PAGE analysis and Western blot analysis demonstrated that PAI-1/GFP gene can be transcripted and translated in rat mesangial cells. PAI-1/GFP gene can express a specific protein. The recombinant PAI-1/GFP had significant inhibition activity on urokinase-type plasminogen activator and displayed autonomous fluorescence. The PA activity of cell supernatants decreased markedly in gene transfer group. FN, LN and Type IV collagen levels were higher than in a control group. CONCLUSION: Over-expression of PAI-1 gene leads to mesangial ECM accumulation. The changes of PA/PAI-1 activity in supernatants of cultured rat mesangial cells might be the key factor in ECM accumulation regulation.

Animals↗
PubMed1999

[The protective effects of thalidomide on acute liver failure].

OBJECTIVE: To understand whether thalidomide (Thal) has the same protective effects as it does in leprosy reaction type II, immunologic and inflammatory diseases. METHODS: D-Galactosamine (D-GalN, 600 mg/kg body weight, intraperitoneally) and lipopolysaccharide (LPS, 5 microg/rat, hypodermically) were used to produce a model of acute liver failure in Wistar rats. The mortality of the models was counted. The plasma peak levels of various cytokines and transaminases were measured. The pathological alterations of the livers were examined with hematoxylin and eosin stain under light microscopy. The expression of tumor necrosis factor (TNF)alpha mRNA was determined by dot blot hybridization combined with semiquantitative analysis. RESULTS: It was found that Thal could lower the mortality of the models (P < 0.05); reduce the peak levels of plasma cytokines, especially TNFalpha, and transaminases (P < 0.05), alleviate the degree of liver injury and suppress the expression of TNFalpha mRNA (P < 0.05). CONCLUSION: It is fully demonstrated that Thal has definite protective effect on acute liver failure, as it does in other diseases. Even though Thal possesses teratogenic effect in patients with pregnancy, it is still believed that Thal has an important curative significance in the treatment of patients who are not pregnant.

Animals↗
PubMed1999

[Morphological cure of cerebral arteriovenous malformations by endovascular therapeutics].

OBJECTIVE: To sum up the clinical characteristics and typical manifestation by analysing clinical materials from total embolized cerebral arteriovenous malformations (AVM) via endovascular embolization. METHODS: We reviewed clinical and image materials of 50 patients whose cerebral AVM were embolized entirely and found the cerebral AVM morphological cure by endovascular embolization after studying the size, position, artery supply, therapeutics and follow-up. RESULTS: 50 patients with cerebral AVM were embolized by endovascular therapy, accounting for 17% of all patients. Malformation lesions were medium or small type, with a diameter less than 3 cm, 95% of them were located in the tentorium superior. Terminal end blood supply was common to AVM and especially medium cerebral artery (MCA) or its branches. 97% of the patients were graded III or below by spectzler grading system. 70% of them had a history of intracranial hemorrhage and were cured by one therapy. CONCLUSIONS: It is a reliable and feasible method for morphological cure in cerebral AVM via endovascular therapeutics only, but case selection is important, that is, medium or small AVM with single branch terminal end blood supply in the tentorium superior.

Adolescent↗
PubMed1999

[The detection of high risk human papillomaviruses in papillary transitional cell carcinoma of urinary bladder].

OBJECTIVE: To investigate the histological evidence of high risk human papillomavirus infection in bladder cancer. METHODS: 52 cases of papillary transitional cell carcinoma of the urinary bladder were studied for the incorporation of high risk human papillomavirus type 16 and type 18 DNA by non-isotopic in situ hybridization (NISH). RESULTS: 19 of 52 cases (36.5%) were positive for HPV DNA with punctuate and/or diffuse purple-blue staining in the nucleus of cancer cells, including two cases positive both for HPV16/18. HPV DNA were predominantly expressed in superficial tumors. Among these positive tumors, adjacent normal epithelium and Brunn's nests also showed positive staining. Morphologically, HPV positive cells existed in the whole layer of papillary tumor cells, 3 of them presented koilocytoid change. CONCLUSIONS: HPV infection might be a common event in urinary bladder and a potential factor for carcinogenesis of bladder cells.

Adult↗
PubMed1999

[Motor-evoked potential in investigating neurologic function during spinal cord ischemia and reperfusion injury].

OBJECTIVE: To investigate the method monitoring the neurologic function of spinal cord ischemia and reperfusion injury. METHODS: Motor-evoked potentials (MEP)were monitored at different time points during ischemia and reperfusion of the lumbar spinal cord of the rat. RESULTS: MEP latency increased 15 minutes after ischemia (P < 0.01) and MEP amplitude decreased 25 minutes after ischemia (P < 0.01). MEP disappeared 40 minutes after ischemia and reappeared 5 minutes after reperfusion, but the latency was still longer and the amplitude was lower than the normal value (P < 0.01). The amplitude returned to normal 2 hours after reperfusion (P > 0.05) in contrast to the latency. 24 hours after reperfusion, the amplitude decreased again, and motor function of rat hind limbs was more decreased than that at early time of reperfusion (P < 0.05). CONCLUSION: MEP is a sensitive index for spinal cord ischemia and reperfusion injury.

Animals↗
PubMed1999

[Responses of normal rat kidney to different ischemic ways].

OBJECTIVE: To evaluate the effects of different ischemic ways on rat kidneys. METHODS: Male Wistar rat kidneys were divided into sham operation (group A), continuous ischemia (group B), intermittent ischemia (group C), ischemic preconditioning (group D), and delayed effect of ischemic preconditioning group (group E). The functional and morphologic changes and levels of MDA of the kidneys which suffered from different ischemia were assessed. RESULTS: The functional indexes of the group C were similar to that of the sham group. In the group B, D and E, the rats showed acute renal failure. Morphological findings showed that the groups B, D and E had severe tubular necrosis (+ + + - + + + +) 24 hours after operation. The group C showed light tubular necrosis (+). 30 minutes after reperfusion, the level of MDA was significantly lower in the group C than in the groups B, D and E (P < 0.01). CONCLUSIONS: In a given ischemic period, the injury effect of intermittent ischemia-reperfusion was much lower than that of continuous ischemia-reperfusion. Experimentally, ischemic preconditioning did not have protective effect on rat kidneys, including "classic protection" and "protection of second window". Intermittent ischemia-reperfusion did not increase OFR and lipid peroxidation injury.

Animals↗
PubMed1999

[Survey on traditional medicinal resources of Uncaria distributed in China].

OBJECTIVE: To make clear the botanical origin of medicinal Uncaria distributed in China and the situation of its use in folk medicines. METHODS: Collecting data and making on-the-spot investigation. RESULTS: There are thirteen medicinal plants which belong to Uncaria genus, and ten of them are commercially available. A clear idea was obtained of their geographical distribution, distribution features and practical experiences in folk application. CONCLUSION: This investigation is helpful in further researching and exploring the resources of Uncaria for medical use, as well as provides a scientific basis for production, management and safe use of crude drugs.

China↗