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Biomedical subjects

Z Yu

Publications and source records attributed to Z Yu.

At least 325 records · Page 18Linked to original sources
PubMed1994

Lysis of porcine trophoblast cells by endometrial natural killer-like effector cells in vitro does not require interleukin-2.

Cells with cytotoxic activity against the cell line K562 and expressing perforin have been demonstrated in endometrial cells isolated from pigs early in pregnancy. This study was designed to determine whether porcine trophoblast cells were susceptible to these endometrial effector cells in vitro. Pregnant gilts (n = 8) were slaughtered between Days 17 and 20 of gestation. Immediately after slaughter, both the endometrial effector cells and trophoblast cells were isolated enzymatically from each animal. Enzymatically dispersed endometrial cells were further fractionated by size at unit gravity, whereas trophoblast cells were enriched by discontinuous density centrifugation on Percoll. Cytolytic activity was evaluated against Na2 51CrO4-labeled trophoblast and K562 cells. Comparison was made between freshly prepared and interleukin-2 (IL-2)-stimulated effector cells. The effect of prostaglandin E2 (PGE2) was tested by including it directly in the 51Cr-release assay. The results indicated that porcine trophoblast cells, like K562 cells, could be recognized and directly lysed by endometrial effector cells. Preculture of effector cells with IL-2 was not required for target lysis but enhanced their cytolytic activity against both trophoblast and K562 targets. In contrast, PGE2 exhibited highly suppressive effects on the cytotoxic activity of both freshly isolated and IL-2-stimulated endometrial effector cells. Conjugate assays demonstrated the binding of trophoblast and K562 targets by effector cells of similar morphology. Cold-target inhibition assays suggested that the effectors in porcine endometrial cell preparations that killed trophoblast and K562 cells were the same NK cell-like population.

Animals↗
PubMed1994

Cellular functions of diabetic cardiomyocytes: contractility, rapid-cooling contracture, and ryanodine binding.

To study the mechanisms of cardiac dysfunction in experimental diabetes, adult rat cardiomyocyte shortening (measured with a video edge-detector system), the sarcoplasmic reticulum (SR) Ca2+ content [assessed by rapid-cooling contracture (RCC) and caffeine contracture (CC)] was examined. Ryanodine binding to the SR Ca(2+)-release channel of myocardium homogenate was also studied. Myocytes from diabetic rats showed depressed shortening (44% decrease compared with controls), reduced maximum rates of shortening and relengthening (58 and 56% decrease, respectively), and prolonged time to peak shortening (47% increase). RCCs and CCs from diabetic cells were 68 and 75% of the control values, respectively. Most of these cardiomyocyte abnormalities were corrected by daily insulin treatment in the diabetic rats. Ryanodine binding parameters indicated that the number of high-affinity binding sites was decreased in diabetic hearts. These data suggest that changes in contractile parameters as measured in diabetic myocytes are in good agreement with data obtained from intact heart or cardiac tissue preparations. Decreased SR Ca2+ content and reduced ryanodine binding sites indicate that the SR functions of storage and release of Ca2+ were depressed. This consequently may cause depressed contraction in diabetic hearts.

Animals↗
PubMed1994

Depressed [Ca2+]i responses to isoproterenol and cAMP in isolated cardiomyocytes from experimental diabetic rats.

Experimentally, diabetic rat hearts are characterized by diminished responses to beta-adrenergic stimulation. Among the aberrant responses are diminished beta-adrenoceptor number and depressed contractile protein activity. In this study, intracellular Ca2+ concentration ([Ca2+]i) was determined by microfluorescence in response to beta-adrenergic stimulation to understand the basis for the changes in the beta-adrenergic pathway in diabetic myocardium. In quiescent myocytes, isoproterenol caused a decrease in [Ca2+]i, which was blocked by timolol and thapsigargin. This suggests that the beta-agonist-induced [Ca2+]i changes are mediated, in part, by sarcoplasmic reticulum Ca-adenosinetriphosphatase. Diabetic myocytes showed a blunted response to isoproterenol, which was reversed by insulin treatment. In electrically stimulated myocytes, isoproterenol and 8-bromo-adenosine 3',5'-cyclic monophosphate (cAMP) increased [Ca2+]i and contraction in a concentration-dependent manner. Electrically stimulated diabetic myocytes demonstrated a depressed maximum [Ca2+]i response to isoproterenol and 8-bromo-cAMP without a change in sensitivity. These data suggest that in addition to alterations in beta-adrenoceptor function there are postreceptor defects in diabetic myocardium that may impair the regulation of [Ca2+]i in diabetic myocardium.

8-Bromo Cyclic Adenosine Monophosphate↗
PubMed1994

[Serological investigation on intrafamilial transmission of HCV infection].

To clarify the intrafamilial transmission of HCV infection, the antibody to HCV was assayed in 124 serum samples from the family members of 60 HCV-Ab (+) index cases (group C), and compared with that from 83 family members of 40 HCV-Ab (-) index patients with hepatitis B (group B), Nine of 124, including 2 parents, 6 spouse and 1 granddaughter, were positive for anti-HCV. The prevalence of anti-HCV was 7.3% (9/124) in total, and 1.85% (2/108) in the subjects who had no history of blood donation. None of 83 was positive for anti-HCV in group B. On the other hand, the prevalence of HBV infection was 25.33% in the group of HCV infection only, and 40.91% in the group with HBV/HCV double-infection or HBV infection. It indicates that the risk of HBV intrafamilial transmission is higher than that of HCV, and the risk of transmission of HCV from mother to infant may be lower than that of sexual transmission.

Adolescent↗
PubMed1994

Diminished luminal release of esophageal epidermal growth factor in patients with reflux esophagitis.

OBJECTIVES: It has recently been demonstrated that human esophageal mucosa, containing numerous submucosal mucous glands, has the ability to elaborate significant amounts of esophageal epidermal growth factor (eEGF). Because of its role in the maintenance of the integrity of the esophageal mucosa, we elected to study the rate of secretion of eEGF in patients with reflux esophagitis (RE), compared with controls, using our newly developed esophageal perfusion model. METHODS: Fourteen healthy asymptomatic volunteers and 14 patients with endoscopically confirmed esophagitis underwent esophageal perfusion with saline, HCl (0.01 M, pH 2.1) HCl/pepsin (0.5 mg/ml of HCl), and ending NaCl solution during four consecutive 8-min perfusion periods. All perfusates were assayed for EGF by RIA (Amersham). Results are expressed as mean +/- SEM. Student's t test was used for statistical analysis. RESULTS: The basal rate of luminal EGF release in patients with RE was 3.78 +/- 0.29 ng/min. This value significantly declined (2.27 +/- 0.27 ng/min; p < 0.001) during mucosal exposure to HCl but was significantly enhanced when the HCl perfusing solution was supplemented with pepsin (4.20 +/- 0.29; p < 0.001 vs. HCl). Introduction of saline during the last perfusion period maintained a rate of luminal EGF release similar to that observed during the initial esophageal perfusion with saline. Luminal release of EGF in patients with RE was significantly lower, compared with corresponding values recorded in controls during perfusion with saline (3.78 +/- 0.29 vs. 14.1 +/- 1.25 ng/min; p < 0.00001), with HCl (2.27 +/- 0.27 vs. 5.95 ng/min; p < 0.0001), with HCl/pepsin solution (4.2 +/- 0.29 vs. 11.7 +/- 1.88 ng/min; p < 0.0001), and during the final perfusion period with saline (3.73 +/- 0.25 vs. 15.1 +/- 1.1 ng/min; p < 0.00001). Therefore, the rate of luminal EGF release in controls was 4-fold, 3-fold, 3-fold, and 4-fold higher than that of patients with RE during perfusion with initial saline, HCl, HCl/pepsin and final saline, respectively. CONCLUSIONS: 1) Decreased esophageal EGF in patients with RE may facilitate the development or delay the healing of mucosal injury. 2) Depletion of EGF from the mucus layer covering the epithelium under the impact of refluxed luminal acid/pepsin may be considered as one of the potential underlying mechanisms leading to damage of the esophageal mucosa during gastroesophageal reflux episodes.

Epidermal Growth Factor↗
PubMed1994

[Atrial specific granules are an intracellular calcium store].

In order to elucidate whether the secretion of atrial natriuretic peptide (ANP) is correlated to the release of calcium (Ca) from the atrial specific granules (ASG), it is necessary to explore whether the ASG contain highly concentrated Ca and how the high Ca is maintained in the ASG. The present study was designed to determine Ca in the ASG with the quantitative electron microscope X-ray microanalysis and Ca(2+)-ATPase with electron microscopic (EM) cytochemical technique. The ultrathin cryosections of rapid frozen fresh rat auricles were used for measuring Ca concentration with a JEM-1200EX electron microscope equipped with a Link AN 10,000 energy dispersive X-ray spectroscope. The measurement showed that the Ca concentration in the ASG was quite high, being 81 +/- 15 mmol/kg (n = 10), comparable with that found in the sarcoplasmic reticulum. With the Ca(2+)-ATPase EM cytochemical technique, the reaction products proved to be deposited on the membrane of the ASG. It was postulated that the Ca(2+)-ATPase on the membrane of ASG pumped Ca2+ out of the cytosol into the ASG and thus maintained a high Ca concentration inside the ASG. Therefore, the ASG might be considered to be a Ca store in atrial cardiocytes.

Animals↗
PubMed1994

Impact of acid and pepsin on human esophageal prostaglandins.

OBJECTIVES: Although the prostaglandin-mediated mucosal protection within the gastric compartment has been well established, its potential role in the maintenance of integrity of the esophageal mucosa in humans has not been explored due to the lack of appropriate methodology. METHODS: We have recently developed an esophageal perfusion catheter, equipped with two balloons, compartmentalizing a 7.5-cm segment of the esophageal lumen. Using this catheter, we studied the impact of the luminal perfusion with saline, HCl (0.01 M, pH 2.1), and HCl/pepsin solutions (0.5 mg/ml) on esophageal luminal release of PGE2 in 21 asymptomatic, presumably healthy volunteers (12 M, 9F; mean age 40 yr). The content of PGE2 in its methyl oximated form was measured by RIA (Amersham, IL), using a novel iodinated label. Results are expressed as mean +/- SEM. Student's t test was used for statistical analysis. RESULTS: Perfusion of the esophageal lumen resulted in continuous release of PGE2 into the perfusate at the rate of 1880 +/- 393 pg/min during the first 8-min perfusion period. During continuation of perfusion with saline, the luminal release of PGE2 was maintained at the rate of 1820 +/- 640 pg/min during the second 8-min perfusion period. This rate declined (although in nonsignificant fashion; p < 0.2) during the third perfusion period, reaching a plateau of 1220 +/- 473 pg/min and maintained during the last (period IV) perfusion period with saline. Introduction of acid during the perfusion period II in the second group of investigated subjects resulted in a rapid and statistically significant decline of the luminal release of PGE2 to the value of 1020 +/- 167 ng/min (p < 0.01). Continuation of esophageal perfusion with acid during the next 8-min perfusion period further diminished the luminal release of PGE2 to the value of 520 +/- 73; p < 0.001. The significant decline in the rate of luminal PGE2 release was still maintained despite the replacement of acid with saline during the ending 8-min perfusion (period IV; 560 +/- 80 ng/min; p < 0.001). Esophageal perfusion with HCl/pepsin solution, in group III subjects, potentiated luminal release of PGE2, reaching the value of 1553 +/- 340 pg/min, which is 3 times higher than the value of PGE2 observed during corresponding perfusion with HCl (period III; p < 0.03). This significant impact of HCl/pepsin solution was still maintained despite the substitution of HCl/pepsin with NaCl during the last perfusion period, and was still significantly higher (1260 +/- 220 pg/min; p < 0.02) than the corresponding value during the ending perfusion with NaCl after HCl (group II). This study for the first time demonstrates that luminal release of PGE2 in humans remains under a significant impact of luminal chemical factors such as acid and pepsin. CONCLUSION: The modulatory effect of acid and pepsin on esophageal mucosal prostaglandin release may play a role in the development of reflux-related mucosal pathology.

Adult↗
PubMed1994

Impairment of salivary epidermal growth factor secretory response to esophageal mechanical and chemical stimulation in patients with reflux esophagitis.

OBJECTIVES: It has been demonstrated recently that salivary epidermal growth factor (sEGF) output in healthy individuals is strongly and significantly influenced by esophageal intraluminal mechanical and chemical stimuli. Therefore, we have studied the impact of intraesophageal mechanical and chemical stressors on the rate of secretion of sEGF in 14 patients with reflux esophagitis (RE), and compared these results with corresponding parameters measured in 14 sex- and age-matched controls. METHODS: EGF was assessed in saliva collected during basal conditions, chewing of parafilm, placement of esophageal tubing, inflation of intraesophageal balloons, and perfusion with NaCl, HCl, and HCl/pepsin solutions. The concentration of sEGF was measured with an RIA kit from Amersham (Arlington Heights, IL). RESULTS: The concentrations of sEGF were (mean +/- SEM) 2.50 +/- 0.32 ng/ml and 2.00 +/- 0.37 ng/ml in basal saliva and during stimulation by chewing the parafilm, respectively. Basal sEGF value appeared to be significantly higher than in controls (2.50 +/- 0.32 vs. 1.90 +/- 0.22 ng/ml, p < 0.05, in one-tailed t test). Placement of intraesophageal tubing resulted in a significant decline of sEGF concentration, compared with parafilm-stimulated conditions (1.25 +/- 0.12 vs. 2.00 +/- 0.37 ng/ml, p < 0.0001) and corresponding tubing-stimulated sEGF value in controls (1.25 +/- 0.12 vs. 1.52 +/- 0.16 ng/ml, p < 0.05). sEGF concentrations after inflation of intraesophageal balloons and subsequent perfusion with initial saline, HCl, HCl/pepsin, and ending saline were also highly significantly lower (1.05 +/- 0.18 ng/ml, p < 0.001; 1.10 +/- 0.20 ng/ml, p < 0.001; 1.10 +/- 0.18 ng/ml, p < 0.001; 1.10 +/- 0.19 ng/ml, p < 0.001; and 1.05 +/- 0.18 ng/ml, p < 0.001, respectively) than sEGF concentration recorded during stimulation with parafilm. Concentrations of sEGF during esophageal perfusion with HCl, HCl/pepsin, and ending saline were also significantly lower than corresponding values in controls (1.10 +/- 0.18 vs. 1.49 +/- 0.11 ng/ml, p < 0.05; 1.10 +/- 0.19 vs. 1.59 +/- 0.11 ng/ml, p < 0.05; and 1.05 +/- 0.18 vs. 1.65 +/- 0.13 ng/ml, p < 0.01, respectively). The rate of sEGF output, which was 1.30 +/- 0.24 ng/min during basal conditions, increased significantly during stimulation with parafilm (2.30 +/- 0.38 ng/min, p < 0.05). Both basal and parafilm-stimulated sEGF outputs were somewhat higher, although nonsignificantly, than corresponding values recorded in healthy individuals. Mechanical and chemical stimulation (initial NaCl, HCl, and ending NaCl) failed to evoke a significant increase in sEGF output over the value observed during parafilm stimulation in patients with RE, although such a significant increase was clearly demonstrated in healthy individuals. Therefore, sEGF output in patients with RE remained significantly lower than corresponding values recorded in controls during an entire mechanical stimulation (2.65 +/- 0.35 vs. 4.60 +/- 0.85 ng/min, p < 0.001, after placement of intraesophageal tubing and 2.80 +/- 0.54 vs. 5.15 +/- 0.70 ng/min, p < 0.001, after inflation of balloons). sEGF output in patients with RE remained also significantly lower than adequate control values during chemical stimulation (3.65 +/- 0.64 vs. 5.20 +/- 0.60 ng/min, p < 0.05, during perfusion with initial saline; 3.70 +/- 0.70 vs. 5.20 +/- 0.60 ng/min, p < 0.05, during perfusion with HCl; 3.70 +/- 0.52 vs. 5.55 +/- 0.72 ng/min, p < 0.01, during perfusion with HCl/pepsin, and 3.30 +/- 0.56 vs. 5.80 +/- 0.86 ng/min, p < 0.001, during ending saline). CONCLUSION: Impairment in sEGF secretion during mechanical and chemical intraesophageal stimulation, mimicking the natural scenario occurring during gastroesophageal reflux, may facilitate the development of esophageal mucosal pathology and delay the healing of already developed mucosal injury.

Adult↗
PubMed1993

The nitrogenase proteins of Rhizobium meliloti: purification and properties of the MoFe and Fe components.

The alfalfa-Rhizobium meliloti symbiosis contributes a major portion of biologically fixed nitrogen to temperate zone forage crop production. Highly-purified molybdenum-iron (MoFe) and iron (Fe) nitrogenase components were obtained for the first time from extracts of R. meliloti bacteroids. Intact bacteroid cells were isolated anaerobically from 100 g quantities of alfalfa nodules following storage in liquid nitrogen. Centrifuged bacteroid extracts showed a marked reduction in specific activity when assayed at protein concentrations less than 1 mg/ml. Both nitrogenase proteins were resolved and purified to homogeneity as determined spectroscopically and by SDS-PAGE. The purified MoFe protein differed in several respects from previously characterized nitrogenase proteins. Saturation of the acetylene-reducing and proton-reducing activities of the R. meliloti MoFe protein required higher relative concentrations of Fe protein than nitrogenase proteins purified from free living diazotrophs. Electron allocation to dinitrogen reduction was sustained at component ratios similar to those present in bacteroid extracts, suggesting that while the observed saturation effects were not detrimental to physiological function in the symbiotic system, overall activity could be enhanced by higher levels of iron protein. Analyses of the MoFe protein gave 22 Fe, 22 labile sulfide and 1.7 Mo atoms per molecular unit of 215 kDa. Dithionite-reduced MoFe protein contained a spin 3/2 iron centre but had a lower visible absorbance at 360 nm than the equivalent Azotobacter chroococcum component. Amino-acid composition indicated a notably lesser tryptophan content, and cysteine content greater than that of the equivalent tetrameric protein of free living diazotrophs. Ratios of acidic and basic residues were similar to other MoFe proteins. Calculation of hydrophobicity and discriminant parameters gave values midway between those expected for soluble cytoplasmic proteins and peripheral membrane associated proteins. ADP was tightly bound by the dithionite-free MoFe protein containing reduced iron-molybdenum cofactor. The R. meliloti iron protein was found to be a 64 kDa homodimer containing a single 4Fe-4S metal centre.

Amino Acids↗
PubMed1993

Circadian rhythm in aspirin (ASA)-induced injury to the stomach of the fasted rat.

Circadian rhythms exist in several gastric parameters affecting ulcerogenesis. This study investigated possible circadian rhythmicity in observed aspirin (ASA)-induced gastric lesions in the rat. In five experiments 183 rats were studied at 14 time points over a 24-hr period. Rats were adapted for three weeks on a 12-hr light schedule, fasted 18 hr, then given oral acidified ASA. One hour later, the rats were sacrificed, the stomachs were removed, stretched flat, photographed, scanned, and measured for percent area of gross lesions in the gastric corpus by computer planimetry. Cosinor rhythmometric analysis showed a significant (P < 0.001) rhythm. Mean of rhythm (MESOR) was 5.60 +/- 0.25% of total corpus. Amplitude was 25.89 +/- 6.07% of MESOR. Peak time (acrophase) was 1909 +/- 0101 hr after lights on. Thus, gastric vulnerability to ASA injury exhibited circadian rhythmicity with peak injury during the dark period in this species.

Animals↗
PubMed1993

Compressive and diametral tensile strengths of current adhesive luting agents.

Strength parameters greatly influence the selection of luting agents. This study compared the compressive and diametral tensile strengths of six classes of new adhesive luting agents (ALAs) with zinc phosphate as the controls. The 11 materials tested were prepared according to the manufacturers' instructions for use as luting agents. Mean compressive and diametral strengths and standard errors were calculated for each luting agent (n = 10). Analysis of variance was computed (p < 0.0011) and multiple comparisons tests were performed. Compressive strengths varied from 41.5 MPa for a hydroxyapatite ALA to 178.5 MPa for a composite resin ALA. Diametral tensile strengths ranged from 8.1 MPa for a hydroxyapatite ALA to 45.1 MPa for a composite resin ALA. Conventional powder-liquid glass ionomer ALAs, an encapsulated glass ionomer ALA, a composite resin-glass ionomer hybrid ALA, and the composite resin ALAs demonstrated significantly greater compressive and diametral strengths than the zinc phosphate cements.

Analysis of Variance↗
PubMed1993

Elevated endometrial natural killer cell activity during early porcine pregnancy is conceptus-mediated.

This study investigated an extended time course of endometrial NK cell activity during gestation and the mechanisms underlying changes in uterine NK cell activity in pigs. Endometrial tissues were collected from cyclic, pseudopregnant and pregnant nulliparous pigs on various days post-estrus, and from pigs 10 days after insemination with seminal plasma or killed spermatozoa. NK effector cells were isolated from each endometrial sample, size fractionated and tested for cytolytic activity against NK target cells (K562) using chromium release assays and immunocytochemically for the frequency of perforin-positive cells. Various cell fractions showed different levels of NK activity and had different proportions of cells expressing perforin. Morphologically, cells in the fraction with maximal NK activity almost all showed typical lymphocyte size and shape. Substantially elevated NK cell activity was recorded in pregnant pigs on days 10 and 20 of gestation. By day 30, the cytolytic activity declined dramatically to an almost undetectable level. Very little activity was found in uterine cells isolated from cyclic, pseudopregnant, and seminal plasma or killed spermatozoa inseminated animals, and no differences were detected either between follicular and luteal phases of the estrous cycle or between different days of pseudopregnancy. These results indicate that elevated NK cell activity during early porcine pregnancy cannot be attributed to contributions from either the maternal systemic endocrine status or from components of boar semen. The changes in NK cell activity observed in porcine endometrial tissues during early pregnancy must therefore be associated with the actual presence of conceptuses.

Animals↗
PubMed1993

Effect of esophageal intraluminal mechanical and chemical stressors on salivary epidermal growth factor in humans.

Although various animal and clinical studies have demonstrated the significant effect of salivary epidermal growth factor (sEGF) on esophageal morphology and function, its secretory patterns still remain inadequately explored. Therefore, we have studied the impact of esophageal mechanical and chemical stimuli on sEGF in humans. sEGF was measured in saliva collected during basal conditions, chewing of parafilm, placement of esophageal tubing, inflation of intraesophageal balloons, and perfusion with NaCl, HCl, and HCl/pepsin solutions. The concentration of sEGF was measured with a radioimmunoassay kit from Amersham (Arlington Heights, IL). The concentration of sEGF in basal saliva was (mean +/- SEM) 2.08 +/- 0.22 ng/ml. Chewing the parafilm resulted in a significant decline of sEGF concentration to the value of 1.39 +/- 0.16 ng/ml (p < 0.0005). Similar decline in sEGF concentration also prevailed after placement of intraesophageal tubing (p < 0.03), and inflation of intraesophageal balloons (p < 0.01). This decline intensified significantly when prolonged esophageal perfusion with saline was implemented (p < 0.03 vs. tubing). Substitution of NaCl with HCl in the second and third perfusion periods prevented the decline in sEGF concentration, whereas HCl accompanied by pepsin enhanced sEGF concentration. The rate of sEGF output was 0.90 +/- 0.13 ng/min during basal conditions and increased significantly during parafilm chewing (1.53 +/- 0.25 ng/min; p < 0.05). However, sEGF secretion during both placement of esophageal tubing and inflation of balloons increased 4.1- and 4.9-fold, respectively (p < 0.002 and < 0.00005), over the basal value, and 2.4- and 2.9-fold, respectively, over the parafilm stimulated secretion. Subsequently, we observed a further significant decline of sEGF output (p < 0.05) which was sustained during perfusion of the esophagus with saline. Interestingly, esophageal perfusion with HCl prevented the decline of sEGF secretion observed during perfusion with saline. sEGF output during esophageal perfusion with HCl/pepsin exhibited a strong increase, reaching the value of 5.86 +/- 0.70 ng/ml. This value corresponds to a 58% increase over the secretory rate observed during mechanical stimulation by placement of esophageal tubing (3.71 +/- 0.47; p < 0.05). HCl/pepsin-induced potentiation of sEGF secretion was also highly significantly increased over both the value recorded during basal (p < 0.0005) and parafilm-stimulated (p < 0.002) conditions. Subsequent substitution of HCl/pepsin solution with a final saline perfusate still maintained enhanced sEGF output, compared with both basal (p < 0.02) and parafilm-stimulated conditions (p < 0.02).(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗