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Biomedical subjects

Z Xu

Publications and source records attributed to Z Xu.

At least 145 records · Page 8Linked to original sources
PubMed2001

[Decomposition processes of Tilia amurensis leaves in the Changbai Mountain forest ecosystem].

A 4-year field experiment was carried out in the Changbai Mountain to study the decomposition process of Tilia amurensis leaf and its affecting environmental factors. The results reveal that the decomposition process of Tilia amurensis leaves could be expressed by an exponent equitation. With the increase of elevation and the change of vegetation types, the decomposition rate in 1-5 standard experimental fields was -0.391, -0.339, -0.257, -0.198 and -0.125, respectively, indicating that the decomposition rate was decreased with ascending elevation. The maximum decomposition took place when the environment was in the shadow of 60%-80%. The leaves were acceleratively decomposed when the tested moisture was increased. Moisture affected the decomposition process more strongly than light.

Biodegradation, Environmental↗
PubMed2001

[Effect of environmental factors on N and P uptake by Gracilaria tenuistipitata var. liui Zhang et Xia].

The effect of light intensity, pH, temperature, salinity on the uptake of nitrogen and phosphorus by Gracilaria tenuistipitata var. liui Zhang et Xia was were studied under laboratory conditions. The uptake rate of nitrogen reached as high as 0.32-0.36 mumol.g-1.h-1 at favorable light intensity of 800-2400 mumol photon.m-2.s-1, while that of phosphorus reached 0.017-0.018 mumol.g-1.h-1 at 320-1600 mumol photon.m-2.s-1. The nutrient uptake rate reached the maximum at pH 8.0. A significant difference of N uptake rate at different temperatures or salinities and that of P uptake rate at different temperatures were found. The N uptake rate was higher at 21-26 degrees C and 20@1000-28@1000, but P uptake was higher at 21-26 degrees C. There was no interaction between temperature and salinity.

Absorption↗
PubMed2001

Hepatic artery angiography and embolization for hemobilia after hepatobiliary surgery.

OBJECTIVE: To evaluate the effectiveness of hepatic angiography and embolization in the diagnosis and treatment of hemobilia after hepatobiliary surgery. METHODS: Nine patients had upper gastrointestinal bleeding 7 days to 3 months after surgery. They underwent emergency hepatic artery angiography and were treated by embolization using Gelfoam particles only (8 patients) and Gelfoam particles plus microcoils (1 patient). RESULTS: Hepatic artery angiography revealed hepatic artery pseudoaneurysms in 3 patients, diffuse hemorrhage of the hepatic artery branches in 3, right hepatic artery-bile duct fistulas in 2, and hepatic artery-small intestine fistula in 1. Hemobilia was controlled with embolization in 7 patients, of whom 1 had recurrent bleeding 1 day after treatment. During the follow-up, 3 patients died of multiple organ dysfunction syndrome. Two patients whose hemorrhage could not be controlled due to technical reasons died several days later. CONCLUSION: When hemobilia after hepatobiliary surgery is suspected, patients should receive hepatic angiography as a first diagnostic procedure and be treated with minimally invasive procedure of selective embolization of the involved artery as soon as possible.

Adolescent↗
PubMed2001

[Role of fibronectin in rat pulmonary fibrosis].

OBJECTIVE: To study the roles of fibronectin (FN) played in rat pulmonary fibrosis. METHODS: The dynamic change of FN was observed in bleomycin-induced rat pulmonary fibrosis using immunohistochemical technique. Northern blot analysis and immunocytochemistry techniques were performed to detect the expression of type I, III procollagen mRNA and their relevant proteins in cultured rat pulmonary fibroblasts (PFbs) after FN treatment. RESULTS: (1) The pulmonary tissue of rats which received bleomycin showed continuously strong positive reaction with anti-FN. (2) The expression of type I, III procollagen mRNA in rat PFbs enhanced at 2 h after FN treatment, and reached their peaks at 6 h, 12 h respectively. The expression of type I, III procollagen proteins detected by immunocytochemistry was also increased after the FN treatment (P < 0.01). Anti-integrin alpha(5) monoclonal antibody could partially inhibit the effect of FN on enhancing the expression of type I, III procollagen in PFbs (P < 0.05). CONCLUSIONS: FN could up-regulate the synthesis of type I, III procollagen mainly by enhancing the transcriptions of procollagen genes.

Animals↗
PubMed2001

[Effects of erythromycin and fosfomycin on Pseudomonas aeruginosa biofilm in vitro].

OBJECTIVE: To observe the effects on Pseudomonas aeruginosa biofilm of non-antipseudomonal drugs such as erythromycin and fosfomycin. METHODS: Clinical isolates of Pseudomonas aeruginosa in respiratory infection and ATCC27853 were cultivated in TSB-Teflon and NS-Teflon system respectively for biolfilm formation. Biofilm was observed under scanning electronic microscope (SEM). MICs were determined by broth micro-dilution. RESULTS: SEM showed that biofilm of clinical isolate in TSB-Teflon system and ATCC27853 in NS-Teflon system increased as being cultivated for longer in 1, 3 and 7 days. And biofilm formation in the process of cultivating was inhibited by SubMIC of erythromycin and fosfomycin. MICs evaluation suggested that there were synergetic effects against biofilm Pseudomonas aeruginosa when SubMIC of erythromycin and fosfomycin were combined with ceftazidime or levofloxacin in vitro. CONCLUSION: SubMIC of erythromycin and fosfomycin could inhibit Pseudomonas aeruginosa biofilm in vitro.

Anti-Bacterial Agents↗
PubMed2001

[Clinico-pathological significance of microvessel density and vascular endothelial growth factor expression in adenoid cystic carcinoma of salivary glands].

OBJECTIVE: To evaluate the clinical significance of microvessel density(MVD) and vascular endothelial growth factor(VEGF) expression in adenoid cystic carcinoma of salivary glands. METHODS: Immunohistochemical staining(SP) was used to quantify MVD and VEGF expression in specimens from 55 patients with adenoid cystic carcinoma of salivary glands. SPSS statistical software was used. RESULTS: High VEGF expression was correlated with tumor site and stage (P = 0.0398, P = 0.0175), but not with local recurrence, distant metastasis, regional lymph node metastasis, histological classification and survival rate. A significant correlation was not noted between MVD and the above variables. The mean MVD in cases of high VEGF expression was significantly higher than those in cases of low VEGF expression (P = 0.0202). CONCLUSIONS: In adenoid cystic carcinoma of salivary glands, MVD and VEGF may be served as a prognostic factor which needs further study, but there is a significant positive correlation between MVD and VEGF expression.

Adult↗
PubMed2001

[Study on variation and interrelations of characteristics at ginseng germplasms].

The variation and interrelations of some characteristics of Panax ginseng germplasms(lines), which were five selfmating generations of some single plants were studied. Methods of multivariate analysis, such as correlation, regression, or clustering, and coefficient of variation were used. The results were of benefit to the breeding and standardized planting of Panax ginseng.

Genetic Variation↗
PubMed2001

Experiences and lessons about soft-tissue flaps covering of severe open tibial fracture.

OBJECTIVE: To sum up experiences and lessons ab out management of soft-tissue reconstruction in open tibial fracture over a 6-year period. METHODS: Twenty-two flap reconstructions were performed to tre at soft-tissue defect of 22 patients with open tibial fracture Type IIIB (Gustilo) from 1993 to 1998. The cases were analyzed and discussed retrospectively aft er follow up of 12-61 months. RESULTS: The size of the flap ranged from 6.6 cm(2) to 28.18 cm(2) and the rate of flap failure was 13.6%. Besides, 3 partial necrosis and 2 postoperative infections occurred in this series. CONCLUSIONS: For soft tissue defect of delayed open tibial fracture Type IIIB, flap reconstruction is still an optimal option. The experiences we obtained are 1) to design a triangular skin extension or a small Z-plasty over the pedicle to reduce the flap tension; 2) to select a unilateral external fixation to provide convenience for any secondary manipulation; and 3) to use serial debridement to diminish flap failure.

Adolescent↗
PubMed2001

[Detection of SYT-SSX fusion gene in paraffin-embedded tissues and its clinicopathologic significance for synovial sarcoma].

OBJECTIVE: To investigate the feasibility of detecting SYT-SSX fusion gene in paraffin-embedded tissues and its diagnostic significance for synovial sarcoma (SS). METHODS: Formalin-fixed, paraffin-embedded samples of 38 cases of SS and 40 cases of control tumors (including spindle cell sarcoma and metastatic adenocarcinoma) were retrived from archival materials. SYT-SSX fusion transcripts were detected in all samples by RT-PCR. House-keeping gene Porphobilinogen Deaminase (PBGD) was used to detect the quality of mRNA. RESULTS: PBGD mRNA was detected in 64 of the 78 tumor cases (82.1%). SYT-SSX fusion transcripts was detected in 33 of the 38 synovial sarcoma specimens. No SYT-SSX mRNA expression was found in control tumors. SYT-SSX mRNA expression rate reached 89.2% (33/37) in synovial sarcomas after exclusion of 1 case which was negative for both SYT-SSX and PBGD. In 33 SYT-SSX positive synovial sarcomas, 22 had SYT-SSX1 and 6 had SYT-SSX2 fusion transcripts. In 5 cases, the fusion type could not be distinguished. There was an association between SYT-SSX fusion type and histologic subtype. All 10 biphasic synovial sarcoma cases had the SYT-SSX1 fusion, whereas 12 of 18 monophasic synovial sarcoma had the SYT-SSX1 and 6 had the SYT-SSX2 fusion gene (P < 0.05). CONCLUSIONS: (1) Detection of SYT-SSX fusion gene in paraffin-embedded tissues was found to be a sensitive and specific method for the diagnosis and differential diagnosis of synovial sarcoma. (2) There was an association between SYT-SSX fusion type and histologic subtype.SYT-SSX2 fusion transcript could only be found in monophasic synovial sarcomas.

Adolescent↗
PubMed2001

[Detection and analysis of SYT-SSX fusion gene in synovial sarcoma].

OBJECTIVE: To demonstrate that a characteristic SYT-SSX fusion gene resulting from chromosomal translocation t (X; 18) (p11; q11) was detectable in archival paraffin-embedded synovial sarcoma tissues. METHODS: SYT-SSX fusion transcripts in 20 synovial sarcomas (histologic subtypes, 15 monophasic and 5 biphasic) were detected and analyzed by the reverse-transcriptase polymerase chain reaction and compared with the results from relevant pathological data. RESULTS: A specific SYT-SSX RT-PCR product was found in 19 of 20 (95%) synovial sarcomas tested and of the 13 tumors containing SYT-SSX2, 10 were monophasic. CONCLUSION: SYT-SSX fusion transcripts are considered as a defining diagnostic marker of synovial sarcomas and the subtypes of SYT-SSX fusion transcripts (SYT-SSX1 and SYT-SSX2) may yield prognostic information.

Adolescent↗
PubMed2001

[Expression of matrix metalloproteinase-2 in rat pulmonary fibrosis].

OBJECTIVE: To study the role of matrix metalloproteinase-2 (MMP(2)) in rat pulmonary fibrosis. METHODS: (1) Dynamic changes of MMP(2) expression were observed in bleomycin-induced rat pulmonary fibrosis by use of immunohistochemical technique. (2) RT-PCR was carried out to show the dynamic changes of MMP(2) mRNA expression of isolated pulmonary fibroblasts (PFbs) during rat pulmonary fibrosis. (3) Northern blot analysis and immunocyto chemistry techniques were performed to detect the expression of MMP(2) mRNA and their relevant protein in cultured rat PFbs after treatment with transforming growth factor 1 (TGF beta(1)). RESULTS: (1) On day 1-7th after receiving bleomycin, the infiltrated pulmonary macrophages and septa mesenchymal cells showed positive reaction with anti-MMP(2) and an increase in number. After 14 days and on 28 th of bleomycin treatment, the number of positive staining macrophages and mesenchymal cells were decreased respectively. (2) The gene expression of MMP(2) mRNA of PFbs were enhanced after bleomycin treatment from the Day 1 and decreased by Day 28. (3) The expression of MMP(2) mRNA and their relevant protein were enhanced in rat PFbs after TGF beta(1) treatment. CONCLUSIONS: Over expression of MMP(2) in pulmonary tissue may be an important factor in the initiation of pulmonary fibrogenesis.

Animals↗
PubMed2001

[Effects of baicalin on hepatocyte apoptosis induced by TNF-alpha and act D in rats].

OBJECTIVE: To study the effects of baicalin on rat hepatocyte apoptosis induced by tumor necrosis factora (TNF-alpha) and actinomycin D (Act D) in vitro. METHODS: Hepatocytes were cultured with different concentrations of baicalin and the cell apoptosis was induced by TNF-alpha and Act D in vitro. After culturing for 24 h, the activity of hepatocysts was examined by MTT assay and the function of hepatocytes for secreting albumin was detected by bromocresolum Viride method. Agarose gelelectrophoresis and flow cytometric analysis were used to determine the apoptotic cells. RESULTS: The actively of the hepatocyte (A) and content of albumin (ALB) in the supernatant of cultured cells in the groups of 0.2 microgram.ml-1 and 2.0 micrograms.ml-1 of baicaline were significantly higher than those in apoptotic model group (A: P < 0.01; ALB: P < 0.01); respectively Even the ALB value in the group of 0.2 microgram.ml-1 baicalin was higher that of black control group (P < 0.01); The clear "ladder-like" streak-band of DNA fragments was found only in the apoptosis model group by the agarose gel electrophoresis. The flow cytometry showed that all the apoptosis rates in the three baicalin groups were lower than that of the apoptotic model group (P < 0.01). CONCLUSION: Baicaline can restrain rat hepatocyte apoptosis induced by TNF-alpha and ActD in vitro.

Animals↗
PubMed2001

[Analysis on characters of 220 cases of tympani membrane perforated].

220 cases of tympani membrane perforated were summarized retrospectively. The tympani membrane perforated of injury was different from otitis media's because they had marked or extreme marked difference in sex, age, ear distinction, shape, position, size, degree of hearing damaged, time of healing, cause of perforating and adhesive substance. This difference was relative to mechanism of tympani membrane perforated. The distinction gist of two sorts of tympani membrane perforated was expounded through comparison and analysis. It would be of great value to clinical forensic medical examination.

Adolescent↗
PubMed2001

[Confirmation of patulous eustachian tube syndrome by Tubo-tymanoaerodynamic graphy].

OBJECTIVE: To compare the advantage of tympanogram, Morimitsu's method and Tubotymanoaerodynamic graphy (TTAG) in the confirmation of patulous eustachian tube syndrome. METHOD: Twenty ears with patulous eustachian tube syndrome diagnosed clinically were selected. The tympanogram, Morimitsu's method and TTAG were examined in these ears and the positive rate was estimated. RESULT: The confirmation of patulous eustachian tube syndrome by tympanogram, Morimitsu's method and TTAG was 5, 12 and 20 ears in 20 ears with patulous eustachian tube syndrome. The positive rate was 25%, 60% and 100% respectively. TTAG also was useful in following-up. CONCLUSION: TTAG is an important method for diagnosis and following-up in patulous eustachian tube syndrome.

Acoustic Impedance Tests↗
PubMed2001

[The curative effect of livostin spray on treating allergic rhinitis of children].

OBJECTIVE: To observe the effect of Livostin spray on children's allergic rhinitis and to search the mechanism of treating allergic rhinitis. METHOD: 113 patients were treated with Livostin spray (Livostin group) or normal saline spray (control group). RESULT: The total efficiency of Livostin group in treating allergic rhinitis is above 95.1% and that of the control group is 25.0%. Initial time of starting effect of Livostin (72.1%) is in 1 minute, and that of the control group (mostly 23.1%) is in 3 minutes. The keeping curative effect time of Livostin spray is mostly (72.1%) above 5 hours and that of the control group is mostly (30.8%) in 3 hours. After 2 weeks, the eosinophilic granulocyte number in nose's secretion of Livostin group is obviously reduced (P < 0.05). CONCLUSION: Livostin is better than control group in relieving symptoms, keeping curative effect and safety, so Livostin is one kind of effective drug in treating children's allergic rhinitis.

Administration, Intranasal↗
PubMed2001

[Application of CT virtual bronchoscope in foreign-body in children's bronchus].

OBJECTIVE: To evaluate the clinical application of CT virtual bronchoscope in foreign-body in bonchus. METHOD: The CT virtual bronchoscope were used in 21 cases with foreign-body in bronchus, all the images and reports were compared with the actual bronchoscopy. RESULT: The reports and images of CT virtual bronchoscope are very agreed with that of actual bronchoscopy. CONCLUSION: CT virtual bronchoscope is a very good complemental method in preoperative diagnosis of foreign-body in bronchus.

Bronchi↗
PubMed2001

[A study on the breeding of new Ganoderma varieties by UV induced mutagenesis].

UV induced mutagenesis of Ganoderma's protoplast was carried out. By crude screen and careful screen, we obtained two mutants, 43020# and 43026$, whose dry weight and polysaccharides contents are higher than original strain. Results of continued cultivation for 10 generation on PDA slants and successive tests of shaking bottles, and successive 3 time pilot scale tests at 3 tons, showed that these two mutants are excellent strains with steady properties, high production and high content of polysaccharides. This study has provided a rapid and effective method for breeding of Ganoderma varieties which is suitable to industrial fermentation.

Breeding↗