[Effect of total alkaloids of Fissistigma oldhamii on cAMP and isolated hearts].
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Biomedical subjects
Publications and source records attributed to Z X Chen.
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AaHI, a proteolytic hemorrhagic toxin from A. acutus venom, contains one g-atom Zn per mole protein and 2 g-atoms Ca per mole protein. AaHI is activated by calcium and slightly inhibited by zinc. When treated with EDTA, AaHI is completely inactivated. When dialyzed against 1,10-O-phenanthroline, 50-80% of the metal content and activities are lost, while 90% of the hemorrhagic and proteolytic activities are retained when dialyzed against 1,10-O-phenanthroline containing 5mM Ca2+. The results suggest that calcium is essential for the hemorrhagic and proteolytic activities. The circular dichroism spectra show that calcium may play an important role in maintaining a proper structure for AaHI. The function of zinc is not clear.
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