[Advances in the research on seroepidemiology of human herpesvirus-6 infections].
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China↗
Biomedical subjects
Z Wu
Publications and source records attributed to Z Wu.
[Comparison of diet, physical activity and serum lipids between Chinese and Japanese: Chengdu-Fukuoka Study].
A detailed diet history was taken from 326 Chinese adults, 116 college students in Chengdu, China, and 300 Japanese adults and 4529 college students in Fukuoka, Japan. Both populations consume high carbohydrate and low fat diet, but the Chinese diet was higher in carbohydrate, cereal energy/total energy ratio and polyunsaturated fatty acids/saturated fatty acids ratio, and lower in protein, fat and animal fat/total fat ratio compared to the Japanese diet. Cholesterol intake was significantly lower in Chinese than in Japanese (P < 0.001). Physical activity index in Chinese was higher than that in Japanese. Serum lipids and lipoprotein cholesterols were measured in the adults of Chinese and Japanese. Except the serum triglycerids level in female, the total cholesterol, TG, high density lipoprotein-cholesterol and low density lipoprotein-cholesterol levels in Chinese adults were lower than those in Japanese adults (P < 0.001). Since cholesterol is the major predisposing factor for coronary heart disease and the results of our study show that a high carbohydrate and low fat diet can be effective in lowering the cholesterol level in blood it will be of interest to see which kind of oriental dietary may be a preventive against coronary heart disease.
Adult↗
A study of time window for oxidative stress associated with myocardial ischemia and reperfusion.
OBJECTIVE: To investigate the time window for oxidative stress occurred in myocardial tissue and associated with reperfusion. METHODS: Thirty nine conscious rabbits were subjected to 0.5 hours (h) of myocardial ischemia and reperfusion for 0 h, 6 h, 24 h and 72 h, respectively, and nine rabbits served as controls. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and myeloperoxidase (MPO), and contents of GSH and malondyaldehyde (MDA) were measured in myocardial tissues taken from ischemic non-necrotic area (INNA), ischemic necrotic area (INA) and non-ischemic area (NIA), respectively. RESULTS: The antioxidant status of INNA was weakened at 6th h, 24th h, and 48th h of reperfusion. Activities of Mn-SOD and Cu-Zu-SOD returned to normal value at 72nd h of reperfusion, but GSH-px activity and GSH content remained in lower value. MDA content had a marked increase at 6th h, 24th h, 48th/h and 72nd h of reperfusion. MPO activity had a progressive increase after reperfusion, with its peak value at 48th h, and PMN accumulation occurred mostly in necrotic zone and its outer board zone. A mild oxidative stress also occurred in non-ischemic tissue, but without obvious neutrophils infiltration. CONCLUSIONS: The time window for oxidative stress associated with myocardial ischemia and reperfusion lasts for at least 72 h, and PMN infiltration is one of the most important factors of delayed myocardial reperfusion injury.
Animals↗
Analysis of Chinese isolates of Trichinella spiralis by molecular biotechnology.
OBJECTIVE: To investigate the relationships among Trichinella spiralis isolates obtained from different hosts and geographical regions in China. MATERIALS AND METHODS: Six isolates of Trichinella spiralis obtained from Changchun, Tianjin, Xi'an, Henan and Yunnan were detected. All the isolates were collected from pigs or dogs. Restriction fragment length polymorphism (RFLP), isoenzyme electrophoresis and soluble protein analysis were used. RESULTS: Distinctive banding patterns in restriction maps were seen between Changchun and other isolates studied. A cloned specific repetitive DNA sequence (1.12 kb) from Changchun isolate was then selected to prepare probe for Southern blotting of DNA fragments. The hybridizing banding patterns among the isolates were different and only the Changchun isolate showed the 1.12 kb band. The Changchun isolate was also distinguishable from the other isolates in isoenzyme patterns of GP1, G6PD, HK, 6PGDH and AK, as well as in isoelectric focusing electrophoresis where the Changchun isolate presented a specific band at 4.1 P1. CONCLUSIONS: There are at least two different biological types of Trichinella spiralis in China and the differences between them may be attributed to different geographical origins and/or different hosts.
Animals↗
[Gene diagnosis for hepatolenticular degeneration by genetic linkage analysis with four short tandem repeat polymorphisms].
OBJECTIVE: To study the clinical application value of genetic linkage analysis for hepatolenticular degenaration (Wilson's disease, WD) with four STR--D13S316, D13S133, D13S301, and D13S314. METHODS: Polymorphisms of the four short STR, of which one (D13S301) was located in WD gene and the other three (D13S316, D13S133 and D13S314) were at flanking sites of the gene, were amplified by polymerase chain reaction, and the alleles indentification was performed with denaturing polyacrylamide gel electrophoresis and used as genetic marker for haplotype analysis. RESULTS: Genetic linkage analysis indicated that among 56 individuals (35 siblings and 21 secondary-relatives) without any clinical symptoms, 26 were WD heterozygotes, 2 presymptomatic and 28 normal. Among three patient previously diagnosed as possible WD patients, one was identified as WD heterozygote and the other two WD patients definitely. CONCLUSION: This genetic diagnosis system based on the four STR polymorphisms is of high clinical application value for distinguishing individuals from WD families.
Adolescent↗
[Progress in the study of endemic diseases in China, 1996].
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Animals↗
[Studies of mast cell-mediated cytotoxicity to hepatoma cells in vitro].
The effects of mast cells (MCs) isolated from rat peritoneal cavity on rat hepatoma cell line (CBRH7919) in vitro were studied with phase contrast microscopy, scanning and transmission electron microscopy. The results showed that different degrees of degeneration were present in all CBRH7919 cells and a few of them exhibited necrosis or disruption when CBRH7919 cells were cocultured with MCs for 24 hours. In situ hybridization demonstrated that the expression of c-myc mRNA in CBRH7919 cells was markedly reduced by MCs. MTT colorimetric assay indicated that the supernatants of MC cultures had suppressive effect on the proliferation of CBRH7919. A monoclonal anti-mouse TNF antibody could decrease the suppressive effect. These results suggest that MCs had anti-tumor effect.
Animals↗
[Research of clinic and laboratory of face acupuncture effect and the exploration of their afferent pathways].
This paper shows that face acupuncture point (Liver point through gallbladder point) has significant effect of promoting bile secretion on the patients of cholecystectomy and choledochotomy and drainage. Special different point is that face acupuncture stimulation has very obvious posteffect comparing with other three body acupuncture groups and this posteffect lasts more than fifty minuts. The results also show that the successful rate of face acupuncture anesthesia is better than body acupuncture anesthesia during the ligation of oviduct and subtotal gastrectomy. The above results are all analysed statistically and there is a very significant difference among them. During the research on domestic dog anesthesiaed by face acupuncture, the successful rate is 86.5%, moreover the analgesic effect of face acupuncture can be blocked by blocking bilateral postganglionic fibre of trigeminal ganglion with 2% procaine. The results imply that trigeminal ganglion is the main afferent way of stimulation information of face acupuncture.
Acupuncture Analgesia↗
[Effect of recombinant human epidermal growth factor on the expansion of human skin in vitro].
We investigated the effect of the different concentrations of recombinant human epidermal growth factor (rhEGF) on the expansion of human skin in vitro. Pieces of skin about 2 mm2 in area were placed in 24-cell wells and rhEGF in different concentrations was added. The wells were incubated at 37 degrees C in 5% CO2 for 4 d. The expanded area of each piece of skin was measured. The results indicated that rhEGF possessed a regulating effect on the expansion of skin pieces, and the expanded area was parabolically related with the concentrations of rhEGF. When the rhEGF concentration reached 5 micrograms/L, the maximal area expansion (7.08 +/- 2.40 mm2) was found, which was as twice as the area in control group (3.63 +/- 1.98 mm2). It was suggested that rhEGF had a potential therapeutic effect in wound healing.
Cell Division↗
[Operative treatment of the endobronchial tuberculosis with cicatricial bronchiarctia].
OBJECTIVE: The experience of surgical treatment in 32 patients with cicatricial brochiarctia by endobronchial tuberculosis was reported. METHOD: The modes of surgical treatment in the series include: sleeve resection was done in 19 cases, lobectomy in 4 cases, pneumonectomy in 7, and left principal bronchial reconstruction in 2 cases. RESULTS: The results were excellent and without operative death. Serious postoperative complication was bronchopleural fistula in one case. The patient was treated by surgical repair of the bronchial stump and buttressed with vascularized pedicle flaps of omentum in the second operation. 2 cases of 32 patients were affected with mild stenosis of bronchial anestomosis after a follow-up of 12 to 18 months. CONCLUSIONS: It was suggested that when the patient developed the endobronchial tuberculosis with cicatricial bronchiarctia, corrective operation should be performed as early as possible, and the sleeve resection should be the first choice.
Adolescent↗
[An experimental and clinical study on radix Salviae miltiorrhiae in the treatment of hepatocellular Ca2+ overload during hepatic ischemia/reperfusion injury].
In recent years there has been considerable interest concerning the role of hepatocellular Ca2+ overload which probably was a major factor in hepatic ischemia/reperfusion injury. We studied the effect of radix salviae miltiorrhizae (RSM) on cytosolic free calciumion concentration [(Ca2+)i](nM) in isolated hepatocytes in rats and patients with ischemia reperfusion by microflurometry using fluorescent (Ca2+)i indicator Fura-2/AM. Changes of lipid peroxide free radical (ROO.) signal ranges with in the liver tissue by ESR technique and those of hepatocellular ultrastructure by electronmicroscope were also observed. The results showed that RSM reduced levels of (Ca2+)i and ROO. Ymax (mm) ESR signal rangs. RSM had an effect on protecting hepatocytes against ischemia/reperfusion injury as a useful receptor-operated calcium channels (ROC) blocker.
Animals↗
[Effect of tetrandrine on calmodulin activity of lung tissue in rats with hypoxic pulmonary hypertension].
OBJECTIVE: The mechanism that tetrandrine (Tet) reduces hypoxic pulmonary hypertension was investigated METHOD: 30 Wistar rats were divided into normal control group, pulmonary hypertension group and tetrandrine-treated group. Total calcium conteins and calmodulin activity in lung tissue were measured with atomic absorption spectrophotometry and phosphodiesterase method. RESULTS: The results showed that Tet could remarkably reduce the mean pulmonary artery pressure and pulmonary vascular resistance in rats with hypoxic pulmonary hypertension, the contents of calcium (62 +/- 10 umol.g-1) and calmodulin (20 +/- 4 ng.mg-1) in the lung were significantly lower in rats administrated with Tet than those (74 +/- 16 umol.g-1, 27 +/- 6 ng.mg-1) rats with hypoxic pulmonary hypertension, and the contents of calcium were related with the mean pulmonary artery pressure and pulmonary vascular resistance in the lung administrated with Tet. CONCLUSION: The results indicated the mechanism that tetrandrine effectively reduces hypoxia pulmonary hypertension may be associated with decreasing intracellular calcium concentration in vascular smooth cells, and the effect may be a result of anti-calmodulin activity.
Alkaloids↗
[A survey on drug abuse in young males in Longchuan County, Yunnan Province].
To understand the prevalence of drug abuse among young people, 1,548 men aged 18 to 29 years were interviewed in 82 natural villages of Longchuan County, Yunnan Province during August to October, 1994, and 433 were found being drug abusers (28%). Most of them started their drug abuse after mid-1980s, and new ones increased yearly. Prevalence of drug abuse in Jingpo ethnic was significantly higher than that in Han and Dai ethnics, and levels of schooling of the people related inversely to their prevalence of drug abuse. Prevalence of drug abuse in young men with smoking, drinking, and premarital or extra-marital sex was much higher than in those without them, and related to their cumulative amounts of smoking and drinking and inversely to their age to start smoking and drinking and to have their first sexual experience. Prevalence of drug abuse in young men who were encouraged to try drugs by others and had family members using drugs was significantly higher than in those without these risk factors. This study suggests drug abuse is prevalent among young men in Longchuan County, and prevention and control of it in adolescents and youngsters should be implemented as soon as possible.
Adolescent↗
External use of bi yuan ning in 200 cases of nasosinusitis and rhinitis.
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Administration, Intranasal↗
[Relationship between the abnormal expression of CD44 gene and bladder cancer].
Reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot hybridization were used to detect exon V6-containing isoforms of CD44 gene in 42 surgical bladder specimens (20 cases of bladder cancer, 10 cases of corresponding normal bladder tissue, 8 non-neoplastic bladder tissue from the benign prostatic hyperplasia, and 4 cystitis). All samples of bladder cancer tissue showed over-expression of many abnormal alternatively-spliced products containing transcripts from exon V6 of the CD44 gene except those from 22 non-neoplastic tissue. Furthermore, the band pattern permitted differentiation between the 2 cases of metastatic bladder tumors and the 18 cases of non metastatic tissue. Our results suggest that there is a remarkable relationship between the abnormal CD44 gene expression (inappropriate splicing and overexpression) and malignant phenotype and metastasis of bladder cancer. The CD44 splice variants may prove to be a more ideal tumor marker for the early diagnosis of bladder cancer and for the early detection of metastatic potential in surgical biopsy specimens, which deserves further evaluation.
Adult↗
[Diagnosis and treatment of iatrogenic ureteral injury].
Diagnosis and treatment of 17 patients who sustained 20 iatrogenic ureteral injuries were analyzed. Primary operations in which ureteral injury occurred are almost gynecologic procedures and general surgery. Injuries were managed by ureteroureterostomy in four, ureteral stent in one, removal suture ligation and ureteral double J-catheter in one, ureteroneocystostomy in ten, psoas-hitch technique and ureteroneocystostomy in one, nephrectomy in two. All patients with appropriate repair were followed-up periods of three to five years. Renal function of these patients had optimal results. When ureteral injury occurs, B-US, IVU, cystoscopy, retrograde ureterography or infusion of dye may be useful. An appropriate repair should be chosen according to length and position of ureteral injuries. Reoperation was optimal in two to three weeks. Immediate recognition of accidental ureteral injury provides optimal results. Patients with unrecognized injuries had suboptimal results.
Adult↗
[Experience of laparoscopic vagotomy for the treatment of chronic duodenal ulcer].
Ten patients with chronic duodenal ulcer underwent posterior truncal vagotomy combined with anterior seromyotomy of lesser curvature of the stomach (Taylor's procedure). Perioperative acid secretory studies (Horlland test) and gastric emptying tests were performed. Postoperative recovery was uneventful in all patients. The acid secretory studies showed decreased BAO, MAO and PAO (P < 0.01). The postoperative gastric emptying was significantly delayed, and the patients are all symptom free. Follow up gastroscopy revealed ulcer healing in 7 patients.
Adult↗
[Determination of color pattern visual evoked potential in early primary open angle glaucoma].
OBJECTIVE: To evaluate the clinical significance of changes of color visual evoked potential (PVEP) in early primary open angle glaucoma (POAG). METHOD: Thirty-one patients (45 eyes) with POAG and fifty-one persons (94 eyes) as age-matched normal controls were tested by color PVEP. RESULTS: In POAG group, the latencies of P100 wave were lengthened, its amplitudes and wave form indexes decreased, the latencies of N145 wave were lengthened, and the amplitudes of N75 wave were lowered. In early POAG eyes without the changes detected by Goldmann perimetry, their color PVEPs were already abnormal. Stepwise regression analysis shows that four factors in color PVEP are correlated to the defect of visual field in POAG. It is proved with the stepwise discriminatory analysis that seven factors in the color PVEP are useful in the diagnosis of early POAG. CONCLUSIONS: Several factors in color PVEP are abnormal in POAG. In early POAG eyes without changes detected by Goldmann perimetry, their color PVEPs are already abnormal. By using discriminatory function, the positive rate of the diagnosis of early POAG tested by color PVEP might be increased.
Aged↗