Biomedical subjects

Z Wu

Publications and source records attributed to Z Wu.

At least 235 records · Page 13Linked to original sources

PubMed2000

Chinese-made electrolytic detachable coil in the embolization of a dog aneurysmal model.

OBJECTIVE: To develop a Chinese-made electrolytic detachable coil (CEDC) that would allow patients to enjoy the benefit of electrolytic detachable coil without having to rely on the expensive Guglielmi detachable coil (GDC), and to test the safety and efficiency of CEDC. METHODS: Radiopacity of the marker of the CEDC was tested. The anti-strain force of coils and connection points were measured. In vitro and in vivo electrolyses were conducted. We produced 21 aneurysm models including lateral, bifurcation, and terminal aneurysm models, and embolized 18 models with CEDC. RESULTS: Radiopacity of the marker was satisfactory. Anti-strain of the coil and the connection point have shown the safety and efficiency of CEDC. Electrolysis in vitro and vivo, was similar to that of GDC. 83% of the aneurysm models were completely occluded. Coils were found to induce thrombosis in the aneurysm cavity, and could lead to a new endothelium over the entrance of the aneurysm, with organized tissue replacing thrombosis in 2 months. CONCLUSIONS: CEDC is as efficient and safe as GDC. The marker, the ability to induce thrombosis inside the sac and the growth of new endothelium over the orifice are very satisfactory.

Animals↗

PubMed2000

[Field epidemiological and experimental study on relationship between genetic factor and nonresponse or hyporesponse to hepatitis B vaccine].

OBJECTIVE: To evaluate the role of genetic factor in the development of nonresponder or hyporesponder to hepatitis B vaccine in Chinese people from field epidemiology and experimental study. METHODS: Six hundred and thirty-four susceptible healthy students were vaccinated with three doses of plasma-derived hepatitis B vaccine. Twenty-nine non- or hypo-responders (subjects) and 30 hyperresponders (controls) as well as their 80 and 79 first degree relatives were determined and recruited in the study. The first degree relatives of subjects and controls were then immunized with hepatitis B vaccine and followed up. On the basis of the above results, alleles of HLA-A, B, C, DR and DQ locus were further detected in the two groups. RESULTS: The non- and hypo-response rate in immunized students was 4.6% (29/634). After administered with hepatitis B vaccine, the first degree relatives of the subjects displayed a 17.9% frequency of nonresponsiveness, higher than in the controls. The phenotype frequency of HLA-DR7 and B54 was 52.2% and 21.7% in subjects, 9.1% and 0.0% higher than in controls, respectively. The extended haplotype B54-DR7 was more frequent in the former (17.0%) than in the latter (0.0%) (P < 0.01). CONCLUSION: These results demonstrated that the genetic factor was likely associated with the non- and hypo-response to hepatitis B vaccine in Chinese people.

Adolescent↗

PubMed2000

Azathioprine and methylprednisolone: prevention of chronic cerebral vasospasm in dogs.

OBJECTIVE: To study the preventive effects of single use of azathioprine and its combination with methylprednisolone on chronic cerebral vasospasm (CVS). METHODS: A canine model of the "double subarachnoid hemorrhage (SAH)" was established to study the angiogram characteristics of chronic CVS. The effects of azathioprine and its combination with methylprednisolone on the prevention of chronic CVS were observed in a randomized study. The content of malondialdehyde (MDA) and pathological changes in the basilar artery (BA) wall were also analyzed. RESULTS: The results were obtained on the 7th day after subcarachnoid hemorrhage. In the azathioprine group, the BA diameter was 87% +/- 26% of the original BA diameter, and the MDA content of BA wall was 0.03 +/- 0.01 nmol/mg tissue. In the azathioprine plus methylprednisolone group, it was 93% +/- 20% (BA diameter) and 0.02 +/- 0.01 nmol/mg tissue (MDA content). In the control group, the BA diameter was 53% +/- 19% and MDA content was 0.11 +/- 0.05 nmol/mg tissue. Pathological examination confirmed the reduction in BA wall damage in the experimental groups. CONCLUSION: Azathioprine has a preventive effect on chronic CVS following SAH. It may suppress the production of free radicals and reduce damage to the BA wall. Combined use of azathioprine with methylprednisolone allows lower dosage and less complication compared with a single agent, and results in a better outcome.

Animals↗

PubMed2000

Changes in systemic and splanchnic hemodynamics after orthotopic liver transplantation in cirrhotic rats.

OBJECTIVE: To investigate early changes in systemic and splanchnic hemodynamics after orthotopic liver transplantation (OLT) in normal and cirrhotic rats. METHODS: Male Sprague-Dawley rats were divided into 4 groups: normal controls (NL, n = 10), intrahepatic portal hypertension (IHPH, n = 10) induced by injection of CCl4, normal rats with OLT (NL-OLT, n = 9) and IHPH rats with OLT (IHPH-OLT, n = 16). IHPH-OLT rats were divided into 2 subgroups: 3 days (Group A, n = 9) and 7 days (Group B, n = 7) after OLT. OLT was performed in rats using cuffs for the anastomosis of the suprahepatic inferior vena cava, infrahepatic vena cava and portal vein. Two weeks after production of IHPH rats, 7 days after NL-OLT rats, 3 days and 7 days after IHPH-OLT rats, radioactive microspheres were used in a hemodynamic study. RESULTS: There were no significant differences in hemodynamic changes between NL-OLT and NL rats, except mean arterial blood pressure (MAP). The characteristics of systemic and splanchnic hyperdynamic circulatory state, including increased cardiac output and splanchnic blood flow, decreased mean arterial blood pressure, total peripheral vascular resistance and splanchnic vascular resistance were observed in IHPH, IHPH-OLT A, and IHPH-OLT B rats. The magnitude of hyperhemodynamics was in the order of IHPH > IHPH-OLT A > IHPH-OLT B rats. Moreover, the derangement of splanchnic hyperhemodynamics was more significant than that of systemic hyperthermodynamics. CONCLUSIONS: The present study demonstrates that the persistence of early systemic and splanchnic hyperkinetic circulation after OLT may be the consequence of factors which maintain hyperhemodynamics in liver cirrhosis, where portal hypertension is not completely eliminated. Hyperhemodynamics is not induced by OLT per se.

Animals↗

PubMed2000

[Construction of adenovirus vector for human p27kip1 gene and its expression].

OBJECTIVE: To study the effects of exogenous p27kip1 gene on cell cycle and proliferation. METHODS: E1 substitutive adenovirus vector pAxlcw containing human p27kip1 cDNA (Cihp27kip1) under the transcriptional control of CMV promoter was constructed and then cotransfected with Ad5 adenovirus DNA-terminal peptide complex EcoT221-digested into 293 cells for preparing p27kip1 recombinant adenovirus Adhp27kip1. After infecting HeLa cells for 24 h, the exogenous p27kip1 protein expression in HeLa cells and its effects on cell cycle and proliferation were determined by Western blot, FACS and MTT assay. RESULTS: The titers of resultant recombinant adenoviruses was 1.7 x 10(9) pfu/ml. p27kip1 protein was over-expressed in HeLa cells infected with p27kip1 recombinant adenovirus for 24 h, resulting in blockage of cell cycle transition from G1 phase to S phase and inhibition of cell proliferation. CONCLUSION: p27kip1 recombinant adenoviruses prepared in this study can efficiently transfer p27kip1 gene into HeLa cells and over-express p27kip1 protein in the infected cells. Adhp27kip1 can be used as an agent for gene therapy.

Adenoviridae↗

PubMed2000

[Comparison of several therapeutic methods for hepatocellular carcinoma with tumor thrombi in portal vein].

OBJECTIVE: To compare the therapeutic effect and significance of different treatment methods for hepatocellular carcinoma (HCC) with portal vein tumor thrombi (PVTT). METHODS: One hundred and forty-seven HCC patients with tumor thrombi in the main portal vein or the first branch of portal vein were divided into four groups. A, conservative treatment group (n = 18); B, hepatic artery ligation (HAL) and/or hepatic artery infusion (HAI) group (n = 18), periodically received postoperative chemoembolizations; C, excision of HCC with removal of PVTT group (n = 79); D, transcatheter hepatic arterial chemoembolization or portal vein infusion (PVI) or HAI after operation group (n = 32). RESULTS: The median survival period was 2, 5, 12, and 16 months in group A, B, C, D, respectively. Their 1-, 3- and 5-year survival rates was 5.6%, 0 and 0 in group A; 22.2%, 5.6% and 0 in group B; 53.9%, 26.9% and 16.6% in group C; 82.8%, 48.8% and 41.3% in group D, respectively. The survival rates differed significantly between the 4 groups (P < 0.05). CONCLUSION: Resection of cancer with removal of tumor thrombi for HCC with PVTT significantly improves the curative effect and quality of life. Local hepatic chemotherapy or chemoembolization after tumor resection with removal of tumor thrombi may further prolong survival period.

Adult↗

PubMed2000

[Effect of recombinant human bone morphogenetic protein-2 on the expression of osteopontin in human periodontal ligament cells].

OBJECTIVE: To investigate the effects of growth factor rhBMP-2 on the expression of osteopontin in periodontal ligament cells(PDLC). METHODS: In culture model of human PDLC, in situ hybridization method was used to detect the expression of osteopontin in human PDLC. RESULTS: The PDLC showed negative reaction to express osteopontin, while those PDLC stimulated by rhBMP-2 showed osteopontin mRNA positive reaction in plasm. CONCLUSION: rhBMP-2 can induce the expression of osteopontin in PDLC. rhBMP-2 may be an effective growth factor in stimulating PDLC differentiation to osteoblast and promote periodontal regeneration.

Bone Morphogenetic Protein 2↗

PubMed2000

[Single cell analysis of H(2)O(2) mediated Fas mRNA expression and Ca(2+) influx in LO(2) cells].

OBJECTIVE: To investigate whether apoptotic injury is induced by Fas mRNA expression mediated by H(2)O(2) and concerned with intracellular Ca(2+) unbalance at a single cell level. METHODS: Fas mRNA expression in human hepatocyte (LO(2) cell) was analyzed by whole-cell patch-clamp combined with single cell reverse transcription polymerase chain reaction (RT-PCR). Cytosolic Ca(2+) concentration was measured with another whole-cell patch-clamp combined with single-cell microfluorescence [Ca(2+)] i measurement at the same time. The index of early phase apoptotic cells (Annexin-V(+) cells) measured with flow cytometry (FCM) and the hepatocytic morphological changes were observed by scanning electron microscopy. RESULTS: Fas mRNA of LO(2) cells cultured with H(2)O(2) (10 micromol/L) was expressed at 2 h. The single cell [Ca(2+)] i analyzed by another whole-cell patchclamp increased to 1 115 nmol/L +/- 227 nmol/L (P < 0.01, t = 13.37); at the same time, an elevated Ca(2+) wave was recorded. The index of Annexin-V(+) cells was as high as 16.18 +/- 0.65 (P < 0.01, t = 48.41), and the membrane surface protuberances were observed. CONCLUSIONS: The activation of Fas mRNA, as a major apoptotic factor mediated by H(2)O(2) in LO(2) cells, is probably associated with the imbalance of intracellular Ca(2+).

Apoptosis↗

PubMed2000

[Apoptosis in atrophic skeletal muscle induced by brachial plexus injury in rats].

OBJECTIVE: To study the role of apoptosis and the expression of apoptosis-associated genes in skeletal muscle atrophy induced by brachial plexus injury in rats. METHODS: Rat models of skeletal muscle atrophy were established by cutting off brachial plexus of one upper limb. Apoptosis of muscular cells was investigated by TUNEL, flowcytometry, DNA electrophoresis and electromicroscopic observation. The apoptosis associated genes such as Fas, FADD, Caspase 8, c-myc, P53 and Bcl-2 were detected by immunohistochemical method (ABC) and Northern-blot. RESULTS: It was found with TUNEL and flowcytometry that the percentage of apoptotic muscle cell rose obviously in atrophic skeletal muscle (P < 0.05). DNA laddering could be seen in DNA gel electrophoresis of atrophic muscle after brachial injury. Morphologic changes of early stage in apoptotic cell could be seen under eletcromicroscope, such as the aggregation of chromosome, the expansion of nucleic cistern and the contraction of nucleus. Fas, FADD and Caspase genes were expressed highly and Bcl-2 gene was expressed lowly with immunohistochemical method in atrophic muscle. The results were all significantly different with that of the controls (P < 0.01). But the expression changes in P53 and c-myc genes were not obvious. The result of Northern-blot indicated that the mRNA of Fas gene rose and that of Bcl-2 gene decreased obviously (P < 0.01) in atrophic muscle induced by brachial plexus injury. CONCLUSION: There are much more apoptotic cells in atrophic muscle induced by brachial plexus injury, and apoptosis plays an important role in its pathogenesis. The apoptotic signal maybe transmitted through Fas-->FADD-->Caspase 8, and the decrease in Bcl-2 gene expression aggravates the process.

Adaptor Proteins, Signal Transducing↗

PubMed2000

Hepatectomy for huge primary liver cancer: report of 171 patients.

OBJECTIVE: To discuss the safety and feasibility of hepatectomy for huge primary liver cancer (PLC). METHODS: The effect of resection of huge PLC was examined retrospectively. Some problems about resection of huge PLC were discussed. RESULTS: In 171 patients with huge PLC undergoing hepatectomy, 2 (1.2%) died in one month after operation. The 1-, 2-, 3-, 5- and 10-year survival rates of the patients were 66.1%, 42.1%, 32.7%, 12.2% and 2.3%, respectively. The effect of prolonging survival time was significant. CONCLUSION: Hepatectomy for huge PLC is safe, feasible, and effective.

Adolescent↗

PubMed2000

[Treatment of non iatrogenic injury of retrohepatic inferior vena cava].

OBJECTIVE: To study a repair method for injured retrohepatic inferior vena cava (IVC) associated with liver trauma. METHODS: Laceration of IVC was sutured bloodlessly after the injured retrohepatic IVC was occluded by compression combined with occlusion of the first hepatic hilus. RESULTS: From April 1996 to January 1999, the injured IVC was successfully repaired in 6 patients. The length of laceration was 2.9 cm (1.5 - 4 cm). The occlusion time was 9.2 min (6 - 15 min) for the IVC and 20.5 min (15 - 29 min) for the first hepatic hilus, respectively. All patients recovered uneventfully, and the IVC was patency without stricture as showed by B-mode ultrasonography and CT. CONCLUSION: The injured retrohepatic IVC can be repaired by direct compression when the laceration of the IVC is caused by liver trauma.

Adolescent↗

PubMed2000

[The affect of Erigeron Breviscapus (Vant.) Hand-Mazz on axoplasmic transport of optic nerve in rats with experimentally elevated intraocular pressure].

OBJECTIVE: To investigate whether Erigeron Breviscapus (Vant.) Hand-Mazz (EBHM) can improve the optic nerve axoplasmic transport in rats with experimentally elevated intraocular pressure (IOP). METHODS: Thirty healthy SD rats were used for the study, acute elevated IOP model in the right eye was built, then they were divided into three groups randomly: Group A (0 day group) included six rats for retinal ganglion cell (RGC) counting via left superior colliculus retrograde horse radish perokidase labeling; Group B, twelve rats divided into EBHM treatment group and control group (6 rats in each subgroup) for RGC counting via left superior colliculus retrograde labeling after twenty days, and Group C included twelve rats submitted the same treatment and procedure as group B after 40 days. RESULTS: After 0 day of acute elevated IOP, no labeled RGCs were observed. After twenty days of acute elevated IOP, in the control and EBHM subgroups the density of labeled RGCs were (423 +/- 220)/mm(2) and (749 +/- 294)/mm(2) respectively, the difference between two subgroups showed statistical significance (P < 0.01). After 40 days of acute elevated IOP, the density of RGCs in the control and EBHM subgroups in group C were (610 +/- 315)/mm(2) and (1,048 +/- 393)/mm(2) respectively, the difference between the two subgroups being statistically significant (P < 0.01). CONCLUSION: After 20 days and 40 days of acute elevation of IOP, the density of RGCs is obviously higher in EBHM group than that in the control group. It is revealed that EBHM can improve the optic nerve axoplasmic transportation blocked by acute elevation of IOP in rats.

Animals↗

PubMed2000

[A case control study on the distribution of apolipoprotein AI gene polymorphisms in the survivors of atherosclerosis cerebral infarction].

OBJECTIVE: To investigate the frequency of the restrict fragment length polimorphisms (RFLP) at 2 MspI sites in the 5' - end of apolipoprotein AI (ApoAI) gene and its potential interaction to atherosclerotic cerebral infarction (ACI) in Chinese Han population. METHOD: Polymerase chain reaction (PCR) technique was used in a sample of 199 cases with documented ACI and 204 healthy matched by age and sex individuals selected from Chinese Han nationality in Beijing. The studied loci include promoter region (-75 bp) and the intron - 1 (+83 bp). RESULTS: (1) Both ACI and control groups M1 + + and M1 + - were major frequent genotypes in Beijng area, M2 + + were major frequent genotypes in intron - 1; (2) M1 + - genotype appeared more common among patients than in controls (0.487 vs 0.368, P < 0.05, OR = 1.64); (3) Individuals with M1 + -/M2 + + haplotype were significantly increased in patients compared with controls (0.437 vs 0.314, P < 0.05, OR = 1.70). CONCLUSION: It is suggested that both M1 + - genotype and M1 + -/M2 + + haplotype of ApoAI gene might associate with ACI in our study.

Apolipoprotein A-I↗

PubMed2000

[The recognition of the epidemic area of tsutsugamushi disease on Nan Peng Lie Island in China and the strategy of prevention].

OBJECTIVE: To investigate the region for the epidemic area of tsutsugamushi disease. METHODS: Epidemiological studies, Orientia tsutsugamushi isolation, and preventive measures were used. RESULTS: The region belonged to epidemic area of south subtropical zone. The main host was found Rattus norvegicu. The main biological vector was Leptotrombidium deliens. The seasonal trends of the quantity of Rattus norvegicu and Leptotrombidium deliens were consistent with the incidence change of the disease in the region. Orientia tsutsugamushi has been isolated from Rattus norvegicu and Leptotrombidium deliens. Data showed that Orientia tsutsugamushi isolated strains mainly belongs to Karp. Sero-epidemiological data showed that antibodies of Orientia tsutsugamushi are 100.0% positive in the residents of the region and 4.0% in army men. After taking comprehensive preventive measures, the incidence was descending. CONCLUSION: Nan Peng Lie island was proved an epidemic area of tsutsugamushi disease.

Animals↗

PubMed2000

[Effect of qi replenishing and blood circulation activating drugs in treatment of middle-advanced pancreatic cancer with radio- and chemotherapy].

OBJECTIVE: To study the effect of Qi replenishing and blood circulation activating drugs (QRBCAD) in treatment of middle-advanced pancreatic cancer with radio- and chemotherapy. METHODS: Patients were divided randomly into two groups, 28 patients treated with radiotherapy and intervention treatment as Group A and 30 patients treated with the same therapy but with additional QRBCAD medication as the Group B, and the effects in the two groups were compared. RESULTS: The short-term effective rate in Group A and B was 53.6% and 67.6% respectively (P < 0.05). The remission rates of jaundice and abdominal pain in Group A were 57.1% and 50.0% respectively, while in Group B, 83.3% and 76.7% respectively, there was significant difference between the two groups, P < 0.05. The gastroenteric reaction occurred in Group B was significantly lesser than that in Group A, P < 0.05. The 1- and 2-year survival rate in Group A were 50% and 21.4%, and in Group B, 80.0% and 46.6% respectively, significant difference (P < 0.05) showed in comparison of the two groups. CONCLUSION: QRBCAD could alleviate the gastroenteric reaction caused by radio- and chemotherapy, raise the clinical symptomatic effective rate of treatment, and prolong the 1- and 2-year survival rate of patients.

Adenocarcinoma↗

PubMed2000

[In vitro studies about the effects of implant materials on calmodulin, introcellular calcium of rat osteoblasts].

OBJECTIVE: 1. To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on the calmodulin (CaM) content of osteoblasts, the concentration of intracellular free calcium and the ATPases activity of cell membrane of rat osteoblasts cultured in vitro; 2. To reveal the biological compatibility of the four implant materials and the index to estimate the biological compatibility condition. METHODS: The powders of HPA, HA, BGC and Ti were sterilized and made into extracted solutions with 199 culture medium. The isolated osteoblasts, which were taken from three-day-old sparague Dawley rats' calvaria, were incubated in 199 culture medium also. Then the osteoblasts were mixed and incubated into the four kinds of extracted solutions of the implant materials. After three days of co-incubation, the cells were taken out, and the cellular calmodulin content were measured with radiobiological analysis. The concentration of intracellular free calcium were under fluorescent analysis, and the ATPases activities of cell membrane were estimated with colorimetric analysis. RESULTS: 1. Calmodulin content of the osteoblasts co-incubated with all the implant materials decreased, and moreover, only that of the Ti group decreased more obviously than that of the Ti and HPA group (P < 0.05). 2. The concentration of intracellular free calcium of all the co-incubated osteoblasts were as normal as that of the control group. 3. Ca(2+)-ATPases activities of osteoblasts co-incubated with HPA, HA, BGC increased (P < 0.05), and Na+/K(+)-ATPases activities of the cells co-incubated with HPA, HA were strenghtened (P < 0.05). CONCLUSION: The indexes of cellular calmodulin content, concentration of intracellular free calcium and ATPases activity of cell membrane can reflect the biological compatibility of implant materials, and they may be used as indexes of estimating biological compatibility condition.

Animals↗

PubMed2000

[Invasion behavior of hepatocellular carcinoma cells correlates with their rheological properties].

We investigated the viscoelasticity, mechanics of adhesion to collagen I coated surfaces and mechanics of adhesin to liver sinosoidal endothelial cells(LECs) among primary culture human hepatocytes, hepatocellular carcinoma(HCC) cells as well as HCC-Inv cells, namely HCC cells capable of invading the artificial basement membrane, Matrigel. The results showed that the above-mentioned rheological properties of HCC cells differed obviously from those of normal hepatocytes. HCC-Inv cells exhibited higher viscoelastic coefficients, higher adhesion forces to collagen I coated surfaces as well as higher adhesion forces to LECs than those of normal hepatocytes and HCC cells. Immunocytochemistry showed the differences in cell morphology and cytoskeleton structure between HCC cells and HCC-Inv cells. A brief discussion was presented in relation to the changes in cell rheological properties of hepatocellular carcinoma cells and their pathological significance.

Carcinoma, Hepatocellular↗

PubMed2000

[Supercritical extraction of Alpinia officinarum and GC-MS analysis of its components].

In order to find the effective components and new pharmaceutical effect of Alpinia officinarum, and further exploit it, we extracted its essential oils with the technique of Supercritical-CO2 fluid, then confirmed there were 27 components in Pot I And 111 components in Pot II by using the chemical component analysis and the computer information retrieval in the GC-MS system.

Alpinia↗