The concentrations of the individual free amino acids were determined in one hour fraction of basal secretion and peak hydrogen ion secretion following stimulation with 2-deoxy-D-glucose (2-DG) (group I) or insulin (group II). Group I consisted of 9 patients with duodenal ulcer having hypersecretion of gastric acid as determined by histamine test; 7 patients with duodenal ulcer who underwent truncal vagotomy and had insulin test performed two weeks after the operation formed group II. The total concentration of free amino acids was similar in basal and in stimulated gastric juice in both groups. Also the concentrations of the individual amino acids did not change significantly after stimulation. There was, however, a significant increase following stimulation in the output of amino acids both in group I and in group II. This increase was parallel to that in the volume of gastric juice, which suggests that a definite amount of free amino acids is always present in the gastric juice, and that the secretion of these acids is not under vagal control.

Amino Acids↗

PubMedDate not supplied

[Monotherapy of epileptic children with monitoring of the serum levels of anticonvulsive drugs].

In 46 children with partial and primarily generalized seizures monotherapy was started with amizepine, luminal, mysodin or phenytoin. The treatment was monitored determining serum concentrations of the drugs. Monotherapy was effective in 74% of cases. In 89% of them the duration of treatment was one year, at least. In some children the values of amizepine, luminal and mysodin concentrations below the therapeutic level were effective. The evolution of electroencephalographic changes failed to correspond strictly to the clinical results of the treatment.

Adolescent↗

PubMedDate not supplied

Gastric acid secretion and the changes of blood gastrin, glucose and insulin levels following 2-deoxy-D-glucose administration in hypersecretory and normosecretory duodenal ulcer patients.

Changes in gastric acid output, as well as the concentration of serum gastrin, blood glucose and serum insulin were measured in hypersecretory and normosecretory duodenal ulcer patients following 2-deoxy-D-glucose (2-DG) administration. The stimulation of gastric acid secretion caused by 2-DG was accompanied by a rise in serum gastrin and blood glucose concentrations. The values of serum gastrin measured during the test were significantly higher in hypersecretors. Despite high glucose level, there was no significant increase in serum insulin concentration both in hypersecretors and in normosecretors.

Adolescent↗

PubMedDate not supplied

Free amino acids of human gastric juice.

The concentration of free amino acids was determined with an amino acid analyzer in the basal and peak secretion fractions of gastric juice obtained from 20 duodenal ulcer patients. 20 amino acids were identified in both fractions, with 5 amino acids, i.e. histidine, glycine, phenylalanine, leucine, and glutamic acid constituting over 50 per cent of the total amino acid concentration. The total concentration of free amino acids in gastric juice, similar in both fractions was lower than in blood serum. The total output of free amino acids was significantly higher in the peak fraction as compared with the basal fraction. The analysis of the results obtained suggests that besides the direct contribution of blood plasma amino acid pool there are two other sources of gastric juice amino acids: secretion from the cells of gastric mucosa and intragastric hydrolysis of proteins and peptides present in gastric juice by concomitantly secreted gastric proteases. No correlation was found between the values of output and concentration of gastric juice amino acids and the correspondent values obtained for hydrogen ion secretion.

Adolescent↗

PubMedDate not supplied

Hydrolysis of milk triglycerides by human gastric lipase.

The concentrations of myristic, palmitic, palmitoleic, stearic and oleic acids were determined in the products of hydrolysis of lipids of cow milk incubated with human gastric juice using thin-layer chromatography for the separation of lipid fractions, and gas liquid chromatography for the determination of fatty acids. It was found that the percentage ratio of the above fatty acids in hydrolysis products was similar to that in milk triglycerides. It was concluded that triglycerides containing higher fatty acids present in milk are hydrolysed by the lipase appearing in human gastric juice, the rate of hydrolysis of the individual acids being roughly proportional to the concentration of these acids in triglyceride substrate.

Animals↗

PubMedDate not supplied

Effect of pentagastrin and 2-deoxy-D-glucose stimulation on the secretion of lipase in human gastric juice.

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Adult↗