A karyometric study of the supraoptic and paraventricular nuclei of the hypothalamus of kainic acid-treated mice.
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Biomedical subjects
Publications and source records attributed to Z Srebro.
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The number of neurocytes containing lipofuscin pigment (LEP) was determined in the nucleus infundibularis dorsalis (NID), the subfornical organ (SFO) and the medulla oblongata (MO) of the brain of sexually mature females and males of Rana temporaria L. approximately identical in length and age. The frogs were captured in their natural habitat in seven periods of the annual cycle. The number of LFP-positive neurocytes shows distinct seasonal variations in the analyzed areas. In general, neurocytes containing LFP were found in higher numbers during the winter months, and their significant drop was observed in summer. The results seem to suggest that cells containing LFP represent a certain kind of dynamic cellular system responding to various internal and/or external factors changing within a year.
The changes in volume of neurocyte nuclei of the subfornical organ (SFO) of Rana temporaria L. under the influence of dehydration were analyzed in the annual cycle. Dehydration conspicuously influenced the activity of the neurocytes of the SFO during the year, depending on the stage of active life on land. It is suggested that in the amphibian under study, SFO participates in the regulation of water balance.
Cytophotometric determinations of cytoplasmic RNA were performed at various hours in normal mice and those given a single injection of mercuric citrate or lead citrate. A cosinor analysis of the results has revealed a marked reduction of the amplitude of the rhythm of RNA content a shift of the acrophase to earlier hours in the experimental animals. Moreover, administration of the tested compounds caused a general reduction of the RNA content in the hypothalami.
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