Biomedical subjects

Z Sheng

Publications and source records attributed to Z Sheng.

113 records · Page 7Linked to original sources

PubMed1987

Hemodilution affects the pressor response to norepinephrine.

A hindlimb preparation of 19 male rats was used to determine the responses to norepinephrine (NE) at different levels of hemodilution at a constant flow, at increased flow and maintained pressure, and after reversal of hemodilution. An initial series demonstrated that the preparation remained stable with unchanged blood pressure, plasma electrolytes, and blood gases; and had reproducible responses to NE throughout the experiment. Two levels of isovolumic hemodilution were achieved by blood/hetastarch exchanges equivalent to 1.5% and 3.0% body weight, which reduced hematocrit to 30.8 +/- 2% and 18.7 +/- 1.5%, respectively. Hemodilution reduced the perfusion pressure from 73.6 mmHg to 58.6 and 55.7 mmHg following 1.5% and 3.0% hemodilution, respectively. A dose-response relationship to NE (0.1, 0.3, 1.0 micrograms/kg) was obtained. The responses to NE were markedly reduced following both 1.5% and 3.0% hemodilution. Retransfusion of packed red blood cells increased the hematocrit from 21.1 +/- 2.2% during hemodilution to 41.2 +/- 2.1% (P less than .01). Concomitantly, perfusion pressure increased from 46.2 +/- 3.6 to 68.3 +/- 6.5 mmHg (P less than .001), close to prehemodilution levels (65.3 +/- 5.3 mmHg). Restoration of hematocrit levels restored responses to NE. In a third series, hemodilution was continued, but the blood flow was increased from 4.0 to 8.3 +/- 0.9 mL/min, and perfusion pressure returned to baseline values. This also restored the pressor responsiveness to NE to near normal.

Animals↗

PubMedDate not supplied

Acidic fibroblast growth factor reduces renal morphologic and functional indicators of injury caused by ischemia and reperfusion.

Therapeutic effects of acidic fibroblast growth factor on postischemic renal injury were evaluated in a rat model of bilateral renal ischemia (60 minutes) and reperfusion (7 days). Twenty-four rats were randomly divided into two groups (12 rats each). After 60 minutes of ischemia and at the onset of reperfusion, rats in the acidic fibroblast growth factor-treated group received 2.6 microg of acidic fibroblast growth factor/rat in 50 microl of phosphate-buffered saline solution containing 0.1% heparin (w/v) through the jugular vein, whereas the rats in the phosphate-buffered saline solution-treated group received the same vehicle without acidic fibroblast growth factor. Compared with the phosphate-buffered saline solution-treated group, rats in the acidic fibroblast growth factor-treated group had significantly lower blood urea nitrogen (83.13 +/- 26.07 versus 176.36 +/- 62.36, p < 0.05) and serum creatinine (0.73 +/- 0.14 versus 1.14 +/- 0.36, p < 0.05) levels 1 day after occlusion. Histopathologic scores showed much less renal damage on day 1 in the acidic fibroblast growth factor-treated rats compared with the phosphate-buffered saline solution controls. We conclude that intravenous administration of acidic fibroblast growth factor offers significant protection against postischemic renal injury and these protective effects may come from its nonmitogenic effects such as the regulation of vessel tone and calcium concentration in the body.

Journal Article↗

PubMedDate not supplied

Ischemia and reperfusion reduce the endogenous basic fibroblast growth factor in rat skeletal muscles: an immunohistochemical study.

Polyclonal antibodies directed against human recombinant basic fibroblast growth factor were used in immunohistochemical studies to localize this growth factor in normal and wounded rat skeletal muscles. According to the intensity of the stain, three main classes of fibers could be identified: the strongly, moderately, and weakly stained fibers. Basic fibroblast growth factor immunoreactivity was found mainly in the extracellular matrix, primarily in the endomysium, which includes the heparin-containing basal lamina, and also in the capillary basal membrane of both normal and wounded muscles; however, the signal intensity was much stronger in normal muscles. The distribution of basic fibroblast growth factor in wounded muscles became markedly heterogeneous and sparse. After 4 hours of ischemia, about 40% of skeletal muscle fibers lost their basic fibroblast growth factor immuno-reactivity. Muscles which underwent 4 hours of ischemia and 24 hours of reperfusion had only a diminished basic fibroblast growth factor immunoreactivity. The pathologic results supported the concept of destroyed cell connection and fiber necrosis in ischemic and reperfused muscles. Potential mechanisms involved in this reduced concentration of basic fibroblast growth factor in wounded muscles may include oxygen free radical activation, a generalized effect of the inflammatory response, and reduced secretion of endogenous basic fibroblast growth factor. These results are only partially compatible with the established mitogenic role of this growth factor and suggest that a reduction of endogenous fibroblast growth factor may partly contribute to a delay in wound healing.

Journal Article↗

PubMedDate not supplied

Epidemiological study of chronic dermal ulcers in China.

A total of 30,000 hospitalized surgical patients in 15 hospitals were screened for chronic ulcers. A total of 489 patients with chronic dermal ulcers were found with their major causes of ulceration including traumatic wounds, infections, diabetes mellitus, and venous diseases. Patients with chronic ulcers following trauma and infection comprised 67.48% of the total patient population. The incidence of diabetic ulcers and venous ulcers was 4.91% and 6.54% respectively. Sites of ulceration differed with different etiological factors. The percentage of chronic dermal ulcers in the lower extremities, upper extremities, thorax and abdomen, back, and head was 63.10%, 17.93%, 7.76%, 4.83% and 6.38% respectively. Of the 489 patients with chronic ulcers, 183 were farmers (37.42%), and 131 were workers (26.79%). Chronic dermal ulcers were more common in men than in women, but there was no significant difference in the sex-related prevalence. According to these data from different hospitals, the incidence of chronic ulcers in patients hospitalized for surgery was 1.5% to 3.0%. These data have primarily shown the prevalence and clinical characteristics of chronic ulcers in hospitalized patients in China. These data may not be consistent with reports from other countries. Significant differences in etiological factors of ulceration, professional distribution of patients with chronic dermal ulcers, and treatment methods were found when compared with reports of studies conducted in developed countries. Our results will benefit not only additional basic research, but these data will also be useful in preventing and managing chronic wounds in developing countries.

Adolescent↗

PubMedDate not supplied

Advances in wound healing research in China: from antiquity to the present.

China is the largest developing country in the world with wounds occuring everyday and everywhere, representing one of the main killers of people. Prevention and treatment of wounds are the main tasks for Chinese surgeons. Although wound care and management have a long history in China, wound healing research and management have made significant progress only in recent years. In this article we give a brief account of the history of wound care and management in China. In addition, we introduce the main research fields and achievements in tissue repair and regeneration during recent times in this country. We hope that these works will benefit our foreign colleagues to know our work and enhance the communication among us.

Animals↗