Biomedical subjects
Z Schwarz
Publications and source records attributed to Z Schwarz.
Nucleotide sequences of five maize chloroplast transfer RNA genes and their flanking regions.
Maize chloroplast tRNA genes encoding tRNA3Ser, tRNAPhe, tRNA2Thr, tRNA2Leu, and tRNAmMet, and their flanking regions have been sequenced. All five gene sequences show features commonly found in the few chloroplast tRNA genes sequenced so far; none of them encodes the 3'-terminal CCA triplet and none of them shows abnormal loop or stem lengths as seen in some genes for mammalian mitochondrial tRNAs. The 5'-flanking regions of some of these tRNA genes contain nucleotide stretches strongly homologous to -35 and -10 promoter regions of bacterial genes. Analysis of these conserved regions together with those seen in other plastid genes yields the consensus sequences ATTGANA at "-35" and TAAGAT at "-10." The 3'-flanking regions of some tRNA genes show dyad symmetries followed by a stretch of Thds. These and other regions may be involved in transcription termination or transcript processing.
Structural and functional analysis of an rRNA operon and its flanking tRNA genes from Zea mays chloroplasts.
The complete analysis of an rRNA operon from Zea mays chloroplasts and its comparison with other plastidic or bacterial rRNA operons is presented. The maize operon contains structural genes for 16S, 23S and 4.5S rRNA species, a leader region proximal to the 16S rRNA gene and a 2.4 kb spacer between the 16S and 23S rRNA genes. Within the spacer DNA sequence are two tRNA genes which code for a tRNAIle and tRNAAla species but each gene is split by large intervening sequences of 949 and 806 bp, respectively. 4.5S rRNA is a structural equivalent of the 3' terminal region of bacterial 23S rRNA. The operon is flanked at its 5' side by a tRNAVal gene and at its 3' side by a 5S rRNA gene. Both these genes are probably not included in the large, primary precursor rRNA transcript.
Electrophoretic elution of nucleic acids from gels adapted for subsequent biological tests. Application for analysis of mRNAs from maize endosperm.
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[Glycolysis and ATP consumption in membrane-free hemolysates].
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