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Biomedical subjects

Z R Chen

Publications and source records attributed to Z R Chen.

At least 37 records · Page 2Linked to original sources
PubMed1989

Immunological studies on thromboangiitis obliterans.

Using humoral immunity (gamma-globulin 18 cases, IgG, IgA, IgM each 30 cases, CH50, C3, C4 each 29 cases and CIC 31 cases), cellular immunity (E-RFC 68 cases, As 30 cases, SmIg, SmIgG each 18 cases, IMIT 30 cases) and immunopathological manifestations (light microscope, fluorescence microscope, transmitted electron microscope each 7 cases) as indices, we studied the immunological changes of "progressing", "remittent" and "stabilized" groups of patients with thromboangiitis obliterans (TAO) in different stages. Humoral immunity indicated that gamma-globulin, immune complex and IgG were all increased; cellular immunity indicated that the rate of T cells and suppressor cells was declined, while that of B cells was elevated. Immunopathologically, under light microscope all the layers of involved vessels were infiltrated with neutrophils, lymphocytes and monocytes; under fluorescence and electron microscope, immune complexes were found in the involved vessel walls. Our preliminary results suggest that TAO is an autoimmune disease relevant to antigen-antibody complex.

Adult↗
PubMed1989

Experimental research on utilization of bioluminescent technique to substitute the current viability count for BCG vaccine.

The possibility of using the bioluminescent (BL) technique to substitute the traditional viability count of colony forming units (CFU) of BCG vaccine was investigated. The results showed there is a significant dose-dependent correlation between the concentration of standard adenosine triphosphate (ATP) and ATP BL value. The ultrasonic-chloroform method designed by us yielded the best results. The correlation coefficient values (r) of BL of the liquid and lyophilized vaccine were found to be 0.8 155 and 0.8 484 respectively (P less than 0.05). The coefficient of variation (CV) between the BL value of different lots of ATP obtained was 3.2-4.4%, much lower than that of CFU (CV = 10.4-11.2%). The presence of bacterial clumps within the vaccine had great influence on the bacterial ATP value and on the CFU viability count. 2.8 fg of ATP was found in each CFU formed in the vaccine with clumps, while it was only 0.94 fg in the vaccine without clumps, indicating the superiority of the BL method. The BL method has shown a high sensitivity, good reproducibility and simplicity in handling with quick results and high accuracy. Therefore, we consider that the BL method can be used to substitute the CFU method.

Adenosine Triphosphate↗
PubMed1989

A multivariate analysis of pathologic factors influencing prognosis of colorectal cancer in 1067 cases.

Multivariate analysis (risk state analysis) was performed to evaluate the single and joint effects of pathologic variables on the survivals of 1067 cases of colorectal carcinoma. Nineteen of the twenty-eight variables analyzed were found to have statistically significant prognostic value, with over half having a strong influence on prognosis. The first seven, ranked in order of their effects on survival, are metastasis in lymph nodes, depth of invasion, histologic grading, lymphocyte infiltration of the cancer, GH (immune response in regional lymph nodes), growth pattern and fibrous proliferation around the cancer. The joint effects of combinations of these major variables are even more striking than those of single ones.

Colorectal Neoplasms↗
PubMed1988

Pharmacokinetics of pholcodine in healthy volunteers: single and chronic dosing studies.

1. The pharmacokinetics of pholcodine after two single doses and after chronic administration were studied in healthy human volunteers. 2. Six subjects received single oral doses of 20 and 60 mg of pholcodine according to a balanced cross-over design with an interval of 3 weeks between the two treatments. Blood and saliva samples and all urine were collected over 168 h after each dosage administration. Subsequently, the same subjects received 20 mg pholcodine 8 hourly orally for 10 days. Blood and saliva samples and all urine were collected during an 8 h dosing interval after the last dose on day 11. 3. Plasma, saliva and urine concentrations of pholcodine were determined by a high performance liquid chromatographic assay. 4. After the single doses, pholcodine was absorbed rapidly (tmax = 1.6 +/- 1.2 h) and eliminated slowly with a mean half-life of 50.1 +/- 4.1 h. The renal clearance of pholcodine was 137 +/- 34 ml min-1 and was inversely correlated with urine pH (r = 0.60) but not with urine flow rate. 26.2 +/- 3.3% of the dose was excreted as unchanged pholcodine after both doses. The concentration of pholcodine in saliva was 3.6 times higher than in plasma. 5. After chronic administration, the pharmacokinetics of pholcodine were not statistically different from the single dose parameters. 6. Pholcodine did not appear to undergo conjugation. The plasma protein binding was 23.5%. Morphine, in unconjugated or conjugated form, was not detected in the urine of any subject after pholcodine administration.

Adult↗
PubMed1988

[Route and preparation of 5-Fu administration as preoperative adjuvant chemotherapy in rectal cancer. II. Morphologic, ultrastructural and histochemical changes of the cancer cells after intrarectal and intravenous 5-Fu administration].

From March 1981 to October 1985, 5-Fu was preoperatively given to 65 Dukes B and C rectal cancer patients (intrarectal suppository 40 and emulsion 20, intravenous 5). The results indicated that after intrarectal administration, marked changes and destruction of the cancer cells in morphology were observed in 40% of the resected rectal specimens for suppository and in 45% for emulsion; marked retrograde degeneration in ultrastructure was found in 47.5% for suppository and in 50% for emulsion; DNA synthesis was obviously reduced in 63% for suppository and in 75% for emulsion. It is suggested that the emulsion be a better preparation. No obvious changes or destruction in morphology and ultrastructure were observed in cancer cells treated by intravenous drip of high dose 5-Fu though leukopenia below 4000 was found in 2/5. However, it was 0/60 by rectal administration. This implies that the intrarectal route is more rational than the conventional intravenous route. This study presents an alternate supplementary treatment in addition to radiotherapy for the reduction of postoperative local recurrence of Dukes B and C rectal cancers.

Administration, Rectal↗
PubMed1982

BCG in China.

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BCG Vaccine↗