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Biomedical subjects

Z Qin

Publications and source records attributed to Z Qin.

At least 91 records · Page 5Linked to original sources
PubMed1993

[Pleural malignant mesothelioma complicated with disseminated intravascular coagulation].

Three cases of MPM complicated with DIC were reported in a total of 6862 autopsies (0.04%) from 1950 to 1989 year in our university hospital. All the three cases showed hemorrhagic patches, shock, decreased platelets and fibrinogen, and prolonged prothrombin and thrombin time. Hyaline thrombosis was observed in the pulmonary interstitial microvessels. We have also discussed the diagnosis and pathogenesis of DIC.

Adult↗
PubMed1993

[Overcoming restriction of Streptomyces hygroscopicus 10-22 by the modification of S. fradiae--an attempt to develop a transformation system for S. hygroscopicus 10-22].

No transformant was obtained when pIJ702 (tsr,mel+) from S. lividans TK24 was used to transform S. hygroscopicus 10-22. pIJ702 isolated from S. fradiae ATCC 10745, however, was transformed into 10-22 at a frequency of 10(3)-10(4) transformants/micrograms DNA. Among the transformant colonies, only 1/1000 of them were black in colour (mel+) while a great majority of them remained white (mel-). Plasraid pIJ702 band was only visualized on agarose gels from the black colonies but not from the white colonies. However, when pIJ702 isolated from both black and white transformants were used to transform S. lividans TK24, the mel gene was expressed normally in the recipients. The preparations was also successful in transforming S. hygroscopicus 10-22, and again gave rise to 1/1000 of black colonies only. When the 10-22 (pIJ702) black colonies were plated on non-selective medium, among the majority of black colonies grown, there were a few white colonies, which were proved to be host mutants of 10-22. These mutants were transformable by pIJ702 and homogeneous black colonies were obtained.

Mutation↗
PubMed1993

[A clinicopathological and immunohistochemical study of 49 cases of necrotizing lymphadenitis].

A clinicopathological and immunohistiochemical study of 49 cases of necrotizing lymphadenitis was made. Twenty-four patients were male and twenty-five female with the M:F ratio of 0.96:1. The patients ranged in age from 9 to 62 years with a mean of 26. Forty-nine patients had superficial lymphadenopathy, and twenty-two were accompanied by fever. The results also showed that there was multifoci necrosis present in the involved lymph nodes, particularly in the cortex or/and paracortex, with variable numbers of small lymphocytes, immunoblasts, histocytes and phagocytes, the latter with phagocytized nuclear debris derived from necrotizing T lymphocytes. However, granulocytes and plasmacytes were generally absent, and B lymphocytes rare. The pathogenesis of this disease remains unknown and the pathogen has not been found by Gram, Giemsa, PAS, Ziehl-Neelsen and Warthin-Starry stain. The diagnosis, differential diagnosis and etiology of this disease are discussed. We support the suggestion that this entity be called "Kikuchi-Fujimoto's disease" rather than "Histiocytic necrotizing lymphadenitis".

Adolescent↗
PubMed1991

Anti-presynaptic membrane receptor antibodies in myasthenia gravis.

Myasthenia gravis (MG) is considered as an autoimmune disease of neuromuscular junction resulting from antibodies directed to acetylcholine receptors (AChR). We describe the use of beta-bungarotoxin (beta-BuTx) and alpha-bungarotoxin (alpha-BuTx) to capture their corresponding proteins from preparation of crude human muscle receptor. beta-BuTx binds to presynaptic membrane receptor (PsmR) of the whole receptor fraction, while alpha-BuTx binds to AchR. The captured proteins were used as antigen in ELISA to detect antibodies to PsmR and to AchR in sera from 82 Chinese patients with MG and in controls. In MG, antibodies to PsmR only were detected in 13%, to AchR only in 11% and both to PsmR and AchR in 54%. Only 3 of 50 patients with other neurological diseases and none of 50 healthy subjects had these antibodies. Specificity tests for antibodies showed that the detection systems which we used are specific and confident. No correlation was found between antibody levels and clinical status. The significance of the PsmR antibodies in the pathogenesis of MG is unknown. We suggest that myasthenia gravis is not only due to damage of the postsynaptic membrane, but of presynaptic structures as well.

Adult↗
PubMed1991

Differential disruption of simple drawing movements in patients with unilateral brain lesion.

In examining the ability of patients with unilateral brain lesion to copy simple drawings of a house and a human face, 18 apoplectic patients confirmed by CT scanning were studied. We found that their drawing of a house was inferior to that of a face in 19 tests (90.5%) of 17 patients (94.4%). Marked differences existed in nearly 90% of these tests. No difference was found between the right-hemisphere and left-hemisphere group, except that more patients of the right-hemisphere group showed contralateral neglect, but in the house drawings only.

Cerebral Cortex↗
PubMed1990

Heterogeneity of mRNA and protein products arising from the protein 4.1 gene in erythroid and nonerythroid tissues.

Immunologically cross-reactive isoforms of the cytoskeletal element protein 4.1 have been identified in many tissues in which they exhibit heterogeneity of molecular weight, abundance, and intracellular localization. To examine the basis for isoform production in erythroid and nonerythroid tissues, we have compared the structure and expression of cDNAs isolated from human erythroid and nonerythroid sources. We have encountered cDNAs representing many distinct mRNA sequences. These exhibit complete nucleotide sequence homology along most of their lengths. Differences were confined to five sequence blocks designated Motifs I-V, which were present or absent in each mRNA moiety. Motif I was expressed only in erythroid cells; it encodes 21 amino acids in a well-characterized spectrin/actin binding domain. Motif II, located near the COOH terminus of the 80-kD "erythroid" protein 4.1 molecule is present in the vast majority of transcripts from both erythroid and nonerythroid cells. Motifs IV and V alter the 5' untranslated region: simultaneous insertion of Motif IV and deletion of Motif V in the untranslated region inserts a new initiator methionine and establishes a contiguous open reading frame encoding a novel 135-kD protein 4.1 molecule. By immunochemical analysis we have identified the longer isoform in cells. Our results are most consistent with tissue-specific alternative mRNA splicing of transcripts of the protein 4.1 gene to yield numerous isoforms. These isoforms exhibit tissue specificity and alter strategic portions of the molecule. Moreover, we describe a novel high molecular weight form of protein 4.1 that arises by splicing events which allow translation at an upstream site.

Amino Acid Sequence↗
PubMed1990

Effect of radix Salviae miltiorrhizae extract injection on survival of allogeneic heart transplantation.

The effect of Radix Salviae Miltiorrhizae (RSM), a commonly used herbal blood circulation invigorator for the treatment of blood stasis in traditional Chinese medicine, on the duration of allograft survival following heterotopic heart transplantation in experimental animals was observed. The results in three heart transplantation models--auricular free graft in mice, abdominal graft in rats and cervical graft in rabbits--suggested that RSM injection in an appropriate dosage prolonged the survival time of cardiac allograft. The herb showed no significant toxicity. It was also found that RSM injection had a synergic effect with corticosteroids against graft rejection.

Animals↗
PubMed1990

[Effect of oxymatrine on prolonging the survival time of cardiac tissue allograft in mice and its immunologic mechanisms].

Oxymatrine is an extract from Sophora flavescens Ait. A daily dose of 75 mg/kg or 225 mg/kg of oxymatrine was given to the recipient intramuscularly for 14 days. The survival time of cardiac tissue allograft was prolonged significantly to 12.2 days (at the dose of 75 mg/kg, P less than 0.05) and 15.7 days (at the dose of 225 mg/kg, P less than 0.001) by oxymatrine, while that in the control group was 10.8 days. The effects of oxymatrine on immune-function in BABL/c mice with or without heart allograft were further studied. Experiments showed that in vitro spontaneous proliferation of spleen cells increased markedly on the 10th day after transplantation, while the proliferation response to Con A of spleen cells decreased. The spontaneous proliferation and proliferation responses to Con A or to LPS of spleen cells could be inhibited significantly in normal mice by oxymatrine. The proliferation response to LPS of spleen cells and RPFC was inhibited obviously in transplanted mice by oxymatrine. However, oxymatrine did not affect the proliferation response to Con A of spleen cells, which had been decreased after transplantation. The results suggested that this drug exhibited selective immuno-suppression on function of B cells without obvious effect on T cell function in transplanted mice. This characteristics of the drug seemed beneficial for avoiding side-effect produced by the conventional immuno-suppressive agents.

Alkaloids↗
PubMed1989

Structure and evolution of mouse interleukin 6 gene.

Restriction fragment length polymorphism in the interleukin 6 gene of murine rodents extending phylogenetically from Mus musculus domesticus to the rat has been analyzed. Most species exhibit distinct restriction site patterns. In contrast, limited polymorphism was found in the tumor necrosis factor alpha gene indicating different selective pressure acting on both genes. The gene encoding interleukin 6 was isolated from a genomic library and the exon/intron organization was determined by restriction analysis and limited DNA sequence analysis. It consists of five exons which distribute over about seven kilobases, thus resembling in structure and organization the human counterpart. Furthermore, no restriction fragment length polymorphisms in the interleukin 6 gene of autoimmune strains NZB, NZW, MRL-lpr/lpr and BxSB could be detected for either EcoRI, BamHI or HindIII.

Animals↗
PubMed1989

[Natural history of spontaneous subarachnoid hemorrhage. A 1-7 year follow-up study of 74 cases].

74 cases of spontaneous subarachnoid hemorrhage (SAH) were followed up for a period of 1 to 7 years, most of them had been examined by CT-scan and/or cerebral angiography. The life-table method was used to determine the cumulative survival rate and cumulative recurrence rate. The mortality rates in both acute and chronic stages and the re-bleeding rate were found to be essentially similar to those of other series of SAH admitted to a tertiary care center as were reported in the Chinese and world literature. Hypertension was probably a risk factor for SAH. The incidence of diabetes mellitus in the present series was significantly higher than that found in the same age groups of general population in Shanghai, which fact deserves further evaluation. The clinical characteristics of the patients in the acute stage were not found to be related to their outcome.

Adolescent↗
PubMed1986

Altered glycosylation of membrane glycoproteins in human uroepithelial cell lines.

Total cellular glycopeptides of 7 human uroepithelial cell lines that differ in the grade of transformation (TGr) were analysed by gel filtration and affinity chromatography on immobilized lectins. The 4 cell lines that are tumorigenic in nude mice and invasive in vitro (TGr III) possess more highly branched, tri- and tetraantennary N-acetyllactosaminic glycans, with less biantennary glycans than the 2 non-tumorigenic, noninvasive (TGr II) cell lines examined. The only exception to this general pattern is the third cell line, which is classified as TGr II. The cellular glycopeptide distribution pattern in this cell line is similar to that of the TGr III cells. The possible relationship between altered glycosylation of membrane glycoproteins and the expression of a malignant phenotype is discussed.

Cell Line↗