Biomedical subjects

Z Pietruska

Publications and source records attributed to Z Pietruska.

At least 19 recordsLinked to original sources

PubMed1998

Changes in soluble IL-6 receptor and IL-6 production by polymorphonuclear cells and whole blood cells of breast cancer patients.

The release of soluble interleukin 6 receptor (IL-6sR) and interleukin 6 (IL-6) was investigated in polymorphonuclear and whole blood cell cultures from untreated patients with breast cancer. IL-6sR and IL-6 were determined in the culture supernatants by a sensitive enzymoimmunological assay. We have shown a decreased ability of unstimulated and stimulated polymorphonuclear cells (PMNs) and whole blood cells (WBC) of cancer patients to release IL-6sR in comparison to a control group. Simultaneously, we have also found an increased capacity of unstimulated cells from the patients to produce of IL-6. The used stimulators-zymosan and lipopolysaccharide (LPS) resulted in increased IL-6sR secretion by PMNs and WBC of patients but did not influence IL-6 production by the same cells. We have also determined the levels of IL-6sR and IL-6 in the sera of cancer patients. The mean concentrations of IL-6sR and IL-6 in the sera of patients were higher than those in the control group. We have not noticed any correlations in the culture supernatant and serum levels of IL-6sR or IL-6 between control and cancer patient groups.

Adult↗

PubMed1997

Release of soluble tumor necrosis factor receptors from polymorphonuclear cells of breast cancer patients.

The release of soluble tumor necrosis factor receptors (sTNF-Rs): soluble tumor necrosis factor receptor I (sTNF-RI) and soluble tumor necrosis factor receptor II (sTNF-RII) by polymorphonuclear cells (PMNs) derived from patients with breast cancer were measured. Serum levels of sTNF-RII and sTNF-RII in patients group were also determined. Significantly higher values of sTNF-RI and sTNF-RII released by PMNs of patients were observed than those in the healthy subjects. There was no significant differences in the serum levels of sTNF-RI and sTNF-RII between breast cancer patients and healthy control. Presented study demostrate that the tumor bearing state, involving breast cancer, may induce an altered soluble cytokine receptors release from the peripheral blood PMNs characterized by increased concentrations of sTNF-RI and sTNF-RII in the culture supernatants of these cells in vitro.

Adult↗

PubMed1997

The ability of human polymorphonuclear cells (PMNS) to release of soluble IL-6 receptor (IL-6sR) in response to different stimuli in vitro.

In the present work we have examined the release of soluble IL-6 receptor (IL-6sR) by polymorphonuclear cells (PMNs) in response to different stimuli: zymosan, ConA, PHA and LPS. We have also investigated the generation of IL-6sR by whole blood cells (WBC) under the same condition of the culture. IL-6sR secreted by PMNs and WBC were detected in cell-free supernatants by ELISA. Results of our investigation revealed that freshly isolated PMNs from healthy subjects exhibit a different ability to the secretion of IL-6sR in response to used stimuli. LPS showed the most significant effect on the IL-6sR secretion by PMNs and WBC as compared to the other inducers. The mean concentrations of IL-6sR released by PMNs were lower than concentrations of IL-6sR released by WBC. Measurements of the IL-6sR secretion by immunocompetent cells appear to be useful in the estimation of the cellular activity mediated by IL-6.

B-Lymphocytes↗

PubMed1996

[Examination of human neutrophil activation in hyperlipidemia].

Chemotaxis, spontaneous migration, phagocytosis, expression of the receptors of Fc Ig fragments and of C3 component of complement has been examined in blood of 51 patients with obesity divided into 3 groups with: 1. disturbances in carbohydrate and lipid metabolism, 2. disturbance in lipid metabolism, 3. no symptoms of metabolic diseases and 20 healthy controls. A comparison of the characteristics of neutral granulocytes in the blood of the healthy controls before and after fat rich meal has been performed. Obtained results indicate that high concentration of lipids, decrease granulocyte activity therefore could be the cause of the decrease of immunological defence.

Adult↗

PubMed1994

Soluble interleukin-2 receptor in serum of patients with esophageal carcinoma.

We measured serum levels of sIL-2R in 28 patients with squamous cell esophageal carcinoma and in 12 controls. Sera for presence of sIL-2R were examined using DAKO Interleukin-2-receptor (CD25) ELISA. The samples were analyzed for CEA and SCC by using IMx System (Abbot). The mean values of sIL-2R in esophageal carcinoma group were significantly increased in comparison with control group. Our results suggest that sIL-2R may be useful in monitoring and prognosis estimation of disease in esophageal carcinoma and could be a valuable addition to the current spectrum of cancer markers.

Adult↗

PubMed1994

Polymorphonuclear leukocytes (PMN) activity in peripheral blood in patients with esophageal carcinoma.

The aim of this study was to evaluate some of the PMN functions connected with phagocytosis in 34 patients with esophageal carcinoma, confronted with a clinical progression of the neoplastic disease, according to TNM classification. The level of the neoplastic markers--SCC and CEA antigens were determined also. In the examined group of patients significant decrease in the mean values of FcR and CR receptors expression, migration, chemotaxis and phagocytosis of PMN were observed. No correlation was found between the percentage cells with FcR and CR and phagocytosis and clinical progression of neoplasma. Significant differences were in mean values of chemotaxis and migration in group with IV stage TNM. There was no found correlation between granulocytic tests and levels of SCC and CEA.

Adult↗

PubMed1993

Complement levels of C4, C3C, factor B, and C1 inactivator in peripheral and retroplacental blood of parturient women with EPH gestosis.

The complement levels were determined in 54 parturient women with EPH gestosis, in 32 normal parturient women and in 24 normal non pregnant women. Complement components: C4, C3c, factor B /Bf/ and C1 inactivator /C1 INA/ were measured by radial immunodiffusion. Peripheral and retroplacental blood were examined. Concentrations of C4, C3c, Bf in peripheral blood of both groups of parturient women were significantly higher than in normal non pregnant women. Peripheral blood of parturient women with EPH gestosis contained significantly higher concentrations of C3c than in normal parturient women. Complement Bf levels of normal parturient women were lower in the retroplacental blood than in the peripheral. The levels of C4 were significantly decreased and C3c increased in the retroplacental blood of parturient women with EPH gestosis when results were compared with retroplacental blood of normal parturient women. Results of present study suggest that complement activation could contribute to the pathogenesis of EPH gestosis.

Complement C1 Inactivator Proteins↗

PubMed1993

[Circulating immune complexes in patients with diabetes type I and II].

The relation between the concentration of immunocomplexes and the number of granulocytes having the surface receptors for Fc immunoglobulin fragment and for C3 component of complement was studied in diabetic patients. The concentration of immunocomplexes was determined with the precipitation method of Swierczyńska or with the method of rosette blocking according to Pazdur et al. The expression of the granulocytic receptors was evaluated by using the modified rosette EA and EAC method according to Buesher. An increase in the concentration of immunocomplexes and a decrease in the percentage of granulocytes having the surface receptors for Fc fragment and C3 protein have been observed in patients with type I and type II diabetes. No correlation was found between the concentration of immunocomplexes and the number of cells with receptors for Fc immunoglobulin fragment and C3 component of complement.

Adult↗

PubMed1991

[Behavior of granulocytic receptors for Fc IgG fragment and C3 component of complement in patients with diabetes].

The count of peripheral neutrophils having the superficial receptors for Fc IgG fragment (FcR) and C3 component of the complement (CR) was determined in diabetic patients by using the rosette tests EA and EAC according to the modified Buescher method. A significantly lower percentage of neutrophils with FcR and CR receptors was found in patients with both insulin-dependent and noninsulin-dependent diabetes. The lowest values were found in noncompensated diabetes; in compensated diabetes they were higher but still significantly lower than in the controls. The difference was significant in both tests used. The results obtained suggest that the observed fall in the percentage of neutrophils with FcR and CR receptors is caused by metabolic disturbances associated with diabetes.

Complement C3c↗

PubMed1989

[Evaluation of the NBT test by cytochemical and spectrophotometric methods].

Reduction of NBT by polymorphonuclear neutrophils was determined with two methods techniques: cytochemical according to Park and spectrophotometrical according to Raman and Poland. 40 tests in healthy men were done. Statistical significance correlation was observed between results obtained using these two techniques. The authors suggest that spectrophotometric technique is easier, more objective and requires less blood for testing.

Adult↗

PubMed1989

[Serum immunoglobulins and various components of complement in patients with insulin-dependent diabetes mellitus].

Serum concentrations of immunoglobulin (Ig) A, M, G and complement components C3, C4, Bf were investigated in 50 patients with insulin--dependent, non--insulin dependent diabetes of 60 patient and control healthy subjects. The mean level of IgA was lower and IgM and IgG higher in both types of diabetic patients. But the low level of IgG was observed in 11% patients with insulin dependent diabetes. It was not significant difference in concentrations of complement components proteins in both diabetic groups and control subjects. Insulin dependent diabetic patients with microangiopathic complications had lower level of serum C4, but this difference was not statistically significant.

Adolescent↗

PubMed1988