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Biomedical subjects

Z Peng

Publications and source records attributed to Z Peng.

At least 73 records · Page 4Linked to original sources
PubMed2000

[Relationship between vulvar dystrophy, malignant tumor and the estrogen progestin receptor].

In order to investigate the relationship between vulvar dystrophy, malignant tumor, and estrogen, progestin receptor, we made an analysis on estrogen and progestin receptor (ER, PR) contents in 31 cases of vulvar dystrophy and 19 cases of malignant tumor, using the dextran-coated charcoal method. The Results showed that ER content of the mixed type was higher than that of the atrophic type. PR content of the mixed type was higher than that of the hyperplastic type; and the PR content of the hyperplastic type was higher than that of the atrophic type. The relationship between ER and PR contents of the vulvar dystrophy and adjacent normal tissue was in positive correlation. The PR content of vulvar squamous cell carcinoma went down along with the drop of its dedifferentiation degree. The PR content of carcinoma was much lower than that of the adjacent normal tissue. The results of suggest that the study provided theoretic basis for endocrinotherapy of the vulvar dystrophy, PR in malignant tumor is regarded as one of the indexes to predict prognosis.

Biomarkers, Tumor↗
PubMed2000

[An adenosine triphosphate bioluminescence assay for detecting the number of living cells].

The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.

Adenosine Triphosphate↗
PubMed2000

[An evaluation of adenosine triphosphate bioluminescence assay for tumor in vitro chemosensitivity testing].

This study was aimed at the feasibility of using ATP-bioluminescence assay for tumor in vitro chemosensitivity testing. With the use of this assay, the authors determined dose-response curve in mouse fibroma cell line L929 treated with chemotherapeutic agents, and investigated the different in vitro responses of 6 ovarian carcinomas (5 from fresh tumor tissues, 1 from ascites) treated with etopside, cis-plating, 5-fluorouracil and adriamycin. The results showed that the coefficients of variation for triplicate assays ranged from 1.2% to 15.8% which means high reproducibility of the assay. The single cell suspension (including < 30 cells clusters) could be separated from tissue fragments by means of enzyme cocktail (collagenase, Dnase, pronase). The viable cells were over 90%. This study demonstrates that ATP-bioluminescence assay is a sensitive, reliable and efficient method for tumor chemosensitivity testing. In this connection, the correlation between in vitro drug sensitivity and in vivo patient response is worth further studying.

Adenosine Triphosphate↗
PubMed2000

[Production and some properties of a thermophilic protease from Bacillus stearothermophilus WF146].

The factors affecting Bacillus stearothermophilus WF146 for thermophilic protease producing have been investigated, more than 600 units of enzyme in 1 mL of fermented culture could be achieved under suitable condition. The protease had a molecular weight around 34 kD estimated by SDS-PAGE, and functioned optimally at pH 8.0 and 80 degrees C, respectively. In addition, the enzyme exhibited high temperature tolerance and was stable at a wide range of pH, and Ca2+ played a key role for the stability of the enzyme. While the protease activity of the enzyme was strongly inhibited by PMSF, DFP and IAA, and was not affected by DTT.

Endopeptidases↗
PubMed1999

Production and characterization of monoclonal antibodies to two new mosquito Aedes aegypti salivary proteins.

Mosquito salivary proteins, which are fundamental to the process of blood feeding, also facilitate disease transmission and cause allergic reactions. The identification and characterisation of these proteins have been hampered by the difficulty of obtaining them in purified form. In this report, we describe the production of mouse monoclonal antibodies (mAbs) against mosquito salivary proteins. BALB/c mice were immunised with Aedes aegypti saliva proteins. Hybridomas were produced by fusion of spleen cells with a mouse myeloma cell line. Positive clones were selected using a saliva-capture ELISA and further identified using immunoblotting. Three mAbs reacted with a 44 kDa protein (Aed a X1) in the saliva-immunoblotting, and did not react with 2 recombinant salivary proteins, rAed a 1 (apyrase) and rAed a 2 (D7), in both immunoblotting and ELISA. Two other mAbs reacted with a 37 kDa protein in saliva-immunoblotting, but failed to react with the 37 kDa rAed a 2 in either immunoblotting or ELISA, suggesting that there is a second 37 kDa protein (Aed a X2) which is recognised by the two mAbs. The 44 kDa and 37 kDa proteins have not been previously identified. These mAbs provide a means to purify proteins, to isolate new genes from the salivary gland cDNA library, and to standardise mosquito extracts, facilitating studies of disease transmission by mosquitoes and of mosquito allergy.

Aedes↗
PubMed1999

Induction of IgE responses using a recombinant mosquito salivary allergen rAed a 2 without adjuvant in mice.

BACKGROUND: Reactions to mosquito bites are a global problem. Several salivary proteins from Aedes (Ae.) aegypti, the most common mosquito species, have been cloned and expressed. Plasmid DNA vaccination has been shown to be effective in the downregulation of IgE responses. To investigate the in vivo antigenicity of these recombinant proteins and to study the mechanisms underlying plasmid DNA vaccination, a mouse model sensitized with a recombinant antigen has been developed. METHODS: BALB/c and C57BL/6 mice were injected intradermally with a 37-kD recombinant Ae. aegypti salivary allergen (rAed a 2) in the absence of adjuvant twice weekly for 8 weeks and then challenged twice with rAed a 2 at weeks 10 and 12. Serum rAed a 2-specific IgE, IgG1 and IgG2a were measured by ELISA. Intradermal tests were performed every 4 weeks. The binding capacity of rAed a 2-specific IgE to the native Aed a 2 was examined by immunoblotting. RESULTS: In both strains, sensitization with rAed a 2 induced a significant increase in IgE and IgG1, but not IgG2a. In all sensitized mice, a positive immediate skin reaction was apparent, while delayed-type hypersensitivity reactions were not observed. BALB/c mice produced significantly higher levels of IgE and IgG1 and larger wheals than C57BL/6 mice. The IgE antibodies elicited by rAed a 2 bound to not only rAed a 2 but also its natural form in mosquito saliva. CONCLUSION: (1) Repeated injections of rAed a 2 without adjuvant induce predominant Th2-type responses in mice. (2) BALB/c mice are better responders for IgE production than C57BL/6 mice. (3) rAed a 2 has identical allergenicity to its natural form.

Adjuvants, Immunologic↗
PubMed1999

Alteration in the mechanical competence and structural properties in the femoral neck and vertebrae of ovariectomized rats.

The structural and mechanical properties of bone in the femoral neck and various other sites were investigated in intact (INT), sham-operated (Sham), and ovariectomized (OVX) rats. Six weeks after operation, the maximal load and energy absorption of the femoral neck were significantly lower in the OVX than in the INT or Sham groups, being 73.2 +/- 1.4 (SE) N, 86.3 +/- 4.1 N, and 87.1 +/- 3.2 N, respectively (p < 0.01) for load. The total cross-sectional area of the femoral neck did not change after OVX, but the marrow cavity area was enlarged, leading to a reduced bone area (including both cortical and trabecular bone) (p < 0.01). Histomorphometric analysis showed that new bone formation could not be detected at the periosteum of the femoral neck below the femoral head, but at the endocortical surfaces the double tetracycline labeling revealed an increased mineral apposition rate (MAR) and bone formation rate (BFR) in OVX animals (p < 0.001). In contrast, MAR and BFR were significantly increased in both periosteal and endocortical surfaces of the tibia, humerus, and femoral shaft, thus preventing a decrease in cortical bone area. The maximal bending loads of the tibia and humerus were not different in the various groups of animals. The correlation coefficient between maximal load and bone area revealed positive relationships in the femoral neck (r = 0.54, p < 0.01), tibia (r = 0.46, p < 0.01), and humerus (r = 0.51, p < 0.01). Ovariectomy resulted in a decreased trabecular bone volume of lumbar vertebra VI (L6) decreased compressive loads of lumbar vertebrae I, III, and IV. These lumbar bone loads were positively related to their L6 bone area (L4/L6: r = 0.66, p < 0.001). Element analyses (energy dispersion spectrometer) from trabecular and cortical areas of bone showed some changes related to aging but not to OVX. These results indicate that ovariectomy influences the biomechanical properties of rat bone by changing structural properties rather than material ones.

Animals↗
PubMed1999

[Comparative study of HLA-DRB1 allele in patients with chronic bronchitis and bronchial asthma].

OBJECTIVE: To make a comparative study of HLA-DRB1 allele frequencies in the cases of chronic bronchitis and bronchial asthma. METHODS: The authors investigated 74 patients with chronic bronchitis and 64 patients with bronchial asthma from among the Han people in Shanxi province. PCR/SSP technique was used for HLA-DRB1 typing, and the patients' data were compared with the normal controls'. RESULTS: The frequency of HLA-DRB1*1201/1202(24.32%) was significantly increased in the chronic bronchitis group(P<0.01),and the frequency of HLA-DRB1*1501/1502(23.44%) was significantly increased in the bronchial asthma group(P<0.05). The frequencies of other DRB1 alleles were not significantly increased in these groups. CONCLUSION: The results indicate that HLA-DRB1*1201/1202 allele is associated with chronic bronchitis and HLA-DRB1*1501/1502 allele is associated with bronchial asthma in the patients among the Hans in Shanxi province.

Alleles↗
PubMed1999

[The contrast between chemotherapy via pelvic retroperitoneal cannulation and intraperitoneal chemotherapy in gynecological malignancies cancers with lymph node metastases].

OBJECTIVE: To contrast the pelvic retroperitoneal (RP) chemotherapy with intraperitoneal (i.p.) chemotherapy in gynecological malignancies with lymph node metastases. METHODS: Fluorouracil (5-FU) was repeatedly injected into the pelvic retroperitoneal space of 43 patients and the abdominal cavity of 11 patients. High performance liquid chromatography was used to determine the drug concentration of 5-FU. 5-FU was repeatedly injected into the pelvic retroperitoneal space of 16 patients via cannulation to observe the nodal 5-FU concentration of injected side and the control side. 99mTc-Sb2S3, Isovist-300 were injected into the pelvic retroperitoneal space of two groups (each one has 3 patients) respectively via cannulation. Single proton emission computer tomography (SPECT) and X-Ray film were taken to observe the allocation of the drugs. RESULTS: (1) Pelvic and periaortic nodal 5-FU concentrations of RP were 60-106 and 76-119 times of that of i.p. respectively. (2) The nodal 5-FU concentrations on repeated injection side were 16-20 times of that on control sides of RP. (3) There was no complications or adverse effect observed. (4) 5-FU was allocated to the space including the external iliac, internal iliac, obturator, deep inguinal and common iliac lymph nodes and also up to the periaortic lymph nodes. CONCLUSION: RP was more effective than i.p. for the treatment of lymphatic metastasis of gynecologic cancer.

Antimetabolites, Antineoplastic↗
PubMed1999

[Phorbol-12-myristate-13-acetate and interferon-gamma synergistic induction of B7-1 expression in ovary carcinoma cells and immunological value].

OBJECTIVE: To investigate the therapeutic potential of phorbol-12-myristate-13-acetate(PMA) and interferon-gamma(IFN-gamma) by induction of expression of adhesion molecule in mucious cystadenocarcinoma of ovary (MCAS) and other tumor cells. METHODS: Expression of costimulatory signals(B7-1), major histocompatibility complex(MHC-I, MHC-II) was analysed with flow cytometry. Cytotoxic T lymphocytes(CTL) killing activity and T cell proliferation induced with expression of B7-1 were evaluated by 4-h 51Cr release assay and standard 3HTdR incorporation and scintitation counting. RESULTS: Pretreatment with PMA and then treated with IFN-gamma was able to enhance B7-1 expression on the tumor cell lines with the constitutive B7-1 expression or on B7-1-transfected tumor cells and to induce B7-1 expression on MCAS and some tumor cell lines without constitutive B7-1 expression. However, treatment with IFN-gamma alone failed to induce B7-1 expression on the tumor cell lines. The synergistic effect of PMA combined with IFN-gamma in the induction of B7-1 expression can be anatagonised by H7 [1-(5-isoquinolinesulfonyl)-2-methyl-Piperazine dihydrochloride]. In addition, treatment with PMA combined with IFN-gamma simultaneously induced or enhanced the expression of MHC class I/II on these tumor cell lines. The tumor cells treated by PMA combined with IFN-gamma were able to induce CTL killing activity and T cell proliferation, which is involved in the expression of B7-1. CONCLUSIONS: The enhancement or induction of B7-1 expression by IFN-gamma may be dependent on the prior activation of protein kinase by PMA and the induced B7-1 expression on tumor cells may have some therapeutic potential.

B7-1 Antigen↗
PubMed1999

[The relationship between angiogenesis and p53 overexpression in endometrioid adenocarcinoma].

OBJECTIVE: To determine the relationship between angiogenesis and p53 overexpression and explore the relationship between angiogenesis and clinicopathologic features in endometrioid adenocarcinoma. METHODS: Angiogenesis was evaluated by intratumoral microvessel density (IMD). Eighty-five endometrioid adenocarcinomas' IMD were identified by immunohistochemical staining of endothelial cells for factor VIII-related antigen, and the expression of p53 protein was stained with the same method by it's monoclone antibody. RESULTS: (1) The positive rate of p53 expression in endometrioid adenocarcinoma was 50.6%. The positive rate in stage III-IV (69.2%) was higher than that in stage I-II(42.4%) (P < 0.05). (2) IMD was higher in stage III-IV (13.27 +/- 6.14) than that in stage I-II (8.31 +/- 4.78) (P < 0.01). IMD in G1, G2 and G3 was 8.95 +/- 5.51, 9.28 +/- 4.87 and 14.73 +/- 7.66, respectively (P < 0.05). In groups of non-myoinvasion, < 1/2 myoinvasion and > or = 1/2 myoinvasion, IMD was 8.45 +/- 5.53, 8.95 +/- 4.99 and 11.96 +/- 6.39, respectively (P < 0.05). (3) In negative, weakly positive and strong positive groups of p53 overexpression, IMD was 5.52 +/- 2.11, 11.75 +/- 4.18 and 16.00 +/- 4.63, respectively (P < 0.01). CONCLUSIONS: Angiogenesis tightly correlated with clinicopathologic factors, such as stage, grade and myoinvasion, is promoted by p53 overexpression in endometrioid adenocarcinoma. It can be concluded that angiogenesis may act as a predictor of prognosis of endometrioid adenocarcinoma.

Adult↗
PubMed1999

[Apoptosis of ovarian carcinoma cell line induced by amiloride].

OBJECTIVE: To investigate induction of apoptosis and its possible mechanism in amiloride-treated ovarian carcinoma cell line. METHODS: Morphological changes of apoptotic cells were investigated by light and fluorescence microscopy. DNA fragmentation was analysed by agarose gel electrophoresis. In addition, the number of hypodiploid cells (apoptotic cells) was quantitatively assessed by flow cytometry and intracellular pH was also analysed. RESULTS: Amiloride-treated ovarian carcinoma cells showed morphological characteristics of apoptosis. A ladder-like pattern of DNA fragmentation was demonstrated on agarose gel electrophoresis. Amiloride at 0.01-5 mumol/L could induce apoptosis in 18.7%-61.6% of ovarian carcinoma cells. The apoptosis-inducing effect of amiloride was dose- and time-dependent. In addition, amiloride induced intracellular acidification in a subpopulation of the treated cells. Furthermore, these isolated acidified cells revealed chromatin condensation as well as DNA degradation with characteristics of apoptosis. There was good correlation between apoptotic cells and acidic cells. CONCLUSION: Amiloride triggers apoptosis of ovarian carcinoma cells; intracellular acidification may be involved in the mechanism of apoptosis.

Amiloride↗
PubMed1999

[Surgical treatment of ovarian malignant tumors metastasized to the gastro-intestinal tract: a report of 131 cases].

OBJECTIVE: To investigate the value and feasibility of surgical excision of gastro-intestinal metastatic tumors in patients with ovarian malignancy. METHODS: The surgical and clinical data were retrospectively reviewed in 131 cases of ovarian malignant tumor with surgically excised gastrointestinal metastasis (> or = 2 cm in diameter). RESULTS: Ovarian malignant tumor with gastro-intestinal metastasis accounted for 28.9% of the patients with primary ovarian malignancies in the same time period. Of the 131 cases, 126(93.9%) were in stage III and IV. The treatment was surgical excision combined with chemotherapy. Of the 141 operations, 83 (58.9%) were done by stripping of tumors on the gastro-intestinal wall and repair, 31(22.0%) received resection of the involved segment of the bowel with anastomosis, 27(19.2%) with colostomies. Postoperative complications occurred in 26.9% of the operated patients. The overall 2- and 5-year survival rate was 79.7% and 42.5%, respectively. CONCLUSION: Surgical excision of gastro-intestinal tumors metastasized from primary ovarion malignancies is feasible to achieve cytoreduction. Prognosis can be improved when combined with chemotherapy.

Adolescent↗
PubMed1999

[Effect of Angelica sinensis injection on CD11c and CD14 expression in alveolar macrophage membrane of chronic bronchitis patients].

OBJECTIVE: To explore the effect of Angelica sinensis injection (ASI) in CD11c and CD14 expression in alveolar macrophage (AM) membrane of chronic bronchitis patients. METHODS: AM from 10 chronic bronchitis patients (remmision stage) and 10 healthy subjects was obtained by bronchoalveolar lavage. After had cultured for 24 hours with ASI or/and lipopolysaccharide (LPS), CD11c and CD14 expression and intracellular calcium ion concentration in AM were analyzed by flow cytometry. RESULTS: CD11c and CD14 expression levels of patients' AM membrane (n = 10) were (39.17 +/- 5.56)% and (35.73 +/- 8.05)% respectively, which were significantly higher than those of the control [n = 10, (16.29 +/- 3.78)% and (15.26 +/- 5.96)%], P < 0.05. LPS could increase the elevation of CD11c expression of patients [(47.25 +/- 7.00)%, P < 0.05], while ASI could reduce the increment [LPS Plus ASI group (27.61 +/- 8.64)%, P < 0.05]. The basic calcium level of AM cytoplasma of patients was (189.47 +/- 23.69) nmol/L (n = 7), which was higher than that of healthy control (99.65 +/- 32.21) nmol/L (n = 6), P < 0.05. The intracellular calcium ion elevation in AM of patients could be induced by LPS, and ASI could inhibit the elevation. The calcium level in LPS group was (288.47 +/- 43.68) nmol/L, in ASI Plus LPS group, (236.68 +/- 28.60) nmol/L, P < 0.05. CONCLUSIONS: ASI could reduce the CD11c expression in AM of chronic bronchitis patients by inhibiting LPS induced intracellular calcium ion elevation in AM, suggesting that ASI may inhibit non-specific inflammation of respiratory tract.

Aged↗
PubMed1999

[A study of Bcl-2 expression in normal endometrium and endometrial carcinoma].

To make an initial inquiry into the correlation of development and prognosis with the expression of Bcl-2 protein in endometrial carcinoma, we detected the Bcl-2 protein expression in 40 normal and 60 malignant samples of the endometrium by means of an immunohistochemical technique. We observed that Bcl-2 expression varied with menstrual cycle, becoming stronger in proliferative phase and then declining in secretory phase. Bcl-2 expression in endometrial carcinoma was much weaker than that in normal endometrium, and Bcl-2 was particularly expressed in adenoacanthoma, papillary adenocarcinoma and simple adenocarcinoma. The higher grade and stage were, the stronger Bcl-2 expression was (P < 0.01). In 5 cases of endometrial carcinoma with hyperplastic endometrium, Bcl-2 expression of carcinoma was obviously weaker than that of hyperplastic endometrium. So we conclude that Bcl-2 overexpression may contribute to the formation of hyperplastic endometrium, that obvious reduction of Bcl-2 expression in endometrial carcinoma may promote apoptosis of cells, and that Bcl-2 over-expression may be associated with good prognosis.

Adenocarcinoma↗
PubMed1999

[A pharmacokinetic study of teniposide in intraperitoneal chemotherapy of ovarian cancer].

The objectives of this study were to determine the characteristics of pharmacokinetics of teniposide (VM-26) instilled intraperitoneally with three dosages (100 mg, 150 mg and 200 mg) and to evaluate its toxicity. Twelve patients with ovarian cancer were divided into three groups: teniposide 100 mg i.p., 5 patients; teniposide 150 mg i.p., 5 patients; and teniposide 200 mg i.p., 2 patients. Samples of ascitic fluid, blood and urine were collected after administration of these drugs in 24 hours. Concentrations of teniposide were determined with high-performance liquid chromatographic procedure. The results showed that the data collected from peritoneum and plasma were found to conform to a two-compartment open model. The peak concentration (Cmax) and the area under the curves (AUC) in peritoneal cavity and plasma were dose-dependent. The ratios of Cmax and AUC between peritoneal fluid and plasma varied within 13.46 +/- 1.89 and 7.65 +/- 2.03 respectively. The elimination half-lives (T1/2 beta) in the peritoneum and plasma of teniposide were 5.28 +/- 0.95 and 6.64 +/- 2.73 hours respectively. The volume of peritoneal distribution and its clearance were lower than those of plasma (P < 0.001). The side effects were mild and were not dependent on drug dosage. The results suggest that teniposide as an intraperitoneal therapeutic agent offers some advantages in the treatment of ovarian cancer. The tumor tissues and peritoneal growths could be bathed in a high concentration of teniposide. Teniposide is safe, reasonable in intraperitoneal chemotherapy and shows prospects in the treatment of ovarian cancer.

Adult↗
PubMed1999

[Microvessel count in normal cervical tissue and in cervical carcinomas].

This study was conducted to determine microvessel count(MVC) status in normal cervical tissue (NCT) and in cervical carcinomas (CCs). 15 cases of NCT and 80 cases of CCs were stained by immunohistochemical method for Factor VIII related antigen (F8RA). The results showed that the MVC in CCs was much higher than that in NCT and it was correlated with clinical stage, size of tumor and lymph node status (P < 0.05). Every time when MVC increased by 5 in number (LM x 200, field 0.739 mm2), the chance of lymph node metastasis increased by 1.61 times. There was no relationship between MVC and tumor grade. These suggested that MVC might be a useful early parameter in the diagnosis and prognosis of CCs.

Carcinoma, Squamous Cell↗