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Z P Vasiurenko

Publications and source records attributed to Z P Vasiurenko.

At least 19 recordsLinked to original sources
PubMed1990

[The role of lipids in the interaction of influenza virus with the host cell].

The published data on the lipids composition of influenza virus, the significance of lipid-protein interactions for support of the virion structure are reviewed. The roles of viral and cellular membrane lipids in the different stages of viral interaction with the host cell: viral adsorption to the cellular surface, penetration into the cell, maturation and assembly of virions are discussed. The data on the significance of lipid composition phenotype of the virus and host cell in realization of the viral infection of the cell are reviewed.

Animals↗
PubMed1989

[The fatty acid composition of Haemophilus and Pasteurella multocida cells as a phylogenetic marker].

When grown on meat-peptone agar with heated blood, different Haemophilus species (H. influenzae, H. parahaemolyticus, H. parasuis, H. pleuropneumoniae), including different H. influenzae serovars (a, b, c, d, e, f), and Pasteurella multocida have identical fatty acid composition, characterized by the prevalence of fatty acids with 16 carbon atoms, constituting about 70% and more of the total number of fatty acids, and a low level of fatty acids with 18 carbon atoms. P. multocida strains cultivated on meat-peptone agar with unheated blood have a greatly increased content of fatty acids with 18 carbon atoms, while the content of fatty acids with 16 carbon atoms is much lower. The identity of fatty acid composition under similar cultivation conditions, together with their similarity in other phenetic signs, is indicative of close phylogenic relationship between bacteria belonging to the genus Haemophilus and P. multocida.

Fatty Acids↗
PubMed1988

[Antibiotic AL-87 induction of changes in the fatty acid composition of phospholipids and neutral lipids in a sensitive strain of Staphylococcus aureus 209P].

Under the action of the sub-bacteriostatic concentration (0.02 microgram/ml) of antibiotic AL-87 there formed in all the fractions of phospholipids and neutral lipids of S. aureus 209P unsaturated branched fatty acids not detected in the control and the content of shorter chain saturated branched fatty acids increased. This means that there were changes leading to increased lipid fluidity. The findings showed that fatty acids of the neutral lipids and phospholipids were involved in regulation of the bacterial cell functional state and participated in this case in providing increased membrane fluidity and permeability.

Anti-Bacterial Agents↗
PubMed1985

[Fatty acid composition of Leptospira lipids as a taxonomic criterion].

The fatty-acid composition of microbial cells in 17 pathogenic and saprophytic Leptospira strains, comprising 14 serovars and 10 serogroups, has been studied. The strains under investigation have proved to fall into 3 groups differing by this characteristic. The group of saprophytic strains is characterized by a comparatively high level of myristic acid and, consequently, by the ratio of saturated and unsaturated fatty acids with 14 carbon atoms approaching 1:1; besides, it is also characterized by a lower, in comparison with the pathogenic Leptospira strains belonging to the serogroups Icterohaemorrhagiae, Canicola, Ballum has a higher level of unsaturated fatty acids. The second group of pathogenic Leptospira strains including the serogroups Grippotyphosa, Hebdomadis, Pomona, Tarassovi, Pyrogenes, Australia has been found to occupy an intermediate position between the first group of pathogenic Leptospira strains and the group of saprophytic ones. As the difference in the content of myristic acid in pathogenic and saprophytic Leptospira strains is a stable characteristic, it can be used for the differentiation of these strains. The present investigation has revealed that the distribution of the main fatty acids in Leptospira phospholipids is similar to their distribution in Leptospira neutral lipids with the exception of unsaturated fatty acid with 14 carbon atoms, occurring mainly in phospholipids.

Chromatography, Thin Layer↗
PubMed1985

[Fatty-acid composition of staphylococci induced by antibiotic AL-87 as a marker of their resistance or sensitivity to the given antibiotic].

Three strains of S. aureus isolated from patients were studied and it was shown that their population was heterogenous with respect to antibiotic AL-87: it contained both the antibiotic sensitive and the antibiotic resistant species in different ratios. The fatty acid composition induced by low concentrations of the antibiotic (subbacteriostatic for the sensitive microorganism) characterized by predominance of branched chain fatty acids in the antibiotic sensitive microorganisms and straight chain fatty acids in the antibiotic resistant microorganisms may serve a marker of sensitivity or resistance. Therefore, the data are indicative of the presence of genetic information in staphylococci determining their resistance to antibiotic AL-87 not yet used in medical practice.

Anti-Bacterial Agents↗
PubMed1984

[Taxonomic significance of the fatty acid composition of bacteria of the genera Bordetella and Haemophilus].

Bacteria of the species B. parapertussis and B. bronchiseptica have proved to be identical in their fatty-acid composition with a high level (35.7-39%) of methylene-hexadecanoic acid, found to be absent in B. pertussis in experimental conditions. At the same time the total content of methylene-hexadecanoic acid and its biosynthetic precursor, hexadecenoic acid, in the first two Bordetella species is similar to the content of hexadecenoic acid in B. pertussis, which, along with the presence of common characteristics in the sign under consideration (the low level of C18:1), indicates the close relationship of these three Bordetella species. Bacteria of the species H. influenzae, H. parainfluenzae, H. aegyptius, H. aphrophilus have similar fatty-acid composition with the prevalence of hexadecanoic and hexadecenoic acids and the low level of fatty acids with 18 carbon atoms. The data on fatty-acid composition may suggest the presence of philogenetic links between the genera Bordetella and Haemophilus.

Bordetella↗
PubMed1984

[Fatty acid composition of variant Staphylococcus aureus 209 P resistant to antibiotic AL-87].

The antibiotic AL-87 resistant variant of S. aureus 209P was grown on the antibiotic-free medium. The study on its fatty acid composition showed that it was analogous to the fatty acid composition of the initial sensitive strain with the predominance of branched fatty acids. However, when the resistant variant was grown on the antibiotic-containing medium, it was characterized by a markedly changed fatty acid composition with the predominance of the straight chain saturated and unsaturated fatty acids, mainly hexadecanoic and octadecenic acids. The fatty acid composition of the resistant variant grown in the presence of the antibiotic was close to that of a wide range of gram-positive and gram-negative microogranisms.

Anti-Bacterial Agents↗
PubMed1983

[Effect of antibiotic AL-87 on the fatty acid composition of microorganisms in different taxonomic groups].

The effect of antibiotic A1-87 on the fatty acid composition of S. aureus 209, E. coli O26 and M. luteus 169 significantly differing in this property was studied. The sub-bacteriostatic doses of the preparation induced the appearance of unsaturated fatty acids in S. aureus 209. These acids were not detected in the control cultures. They also significantly increased the content of the saturated branched fatty acid with 15 carbon atoms in this culture and decreased the content of the fatty acid of the same type with 19 carbon atoms. In E. coli O25 there was an almost two-fold increase in the content of the unsaturated straight chain fatty acids with a respective decrease in the content of cyclopropanoic acid and a markedly pronounced decrease in the content of nonadecanoic acid. In M. luteus 169 the content of the saturated branched fatty acid with 15 carbon atoms increased, while the content of the unsaturated straight chain fatty acids (C16:1, C18:1) decreased, the content of hexadecanoic acid being decreased almost two times. According to the present status the differences in the fatty acid composition of the above organisms are interpreted as one of the mechanisms increasing the membrane permeability.

Anti-Bacterial Agents↗
PubMed1983

[Fatty acid composition of the lipopolysaccharides of bacteria in the genera Escherichia, Shigella and Salmonella as a taxonomic trait].

The comparative study of the fatty acid composition of bacterial lipopolysaccharides (LPS) in the genera Escherichia, Shigella, Salmonella has been carried out under identical experimental conditions. The LPS of the bacteria under study have been found to contain a number of saturated fatty acids and oxyacids which could not be previously detected in these bacteria in other studies. In all bacterial strains under study LPS include mainly 3-oxytetradecanoic, tetradecanoic and dodecanoic fatty acids. The essential feature of the fatty acid composition of Salmonella is the presence of 2-oxytetradecanoic acid; this acid is absent in Escherichia and Shigella, which can thus be used as a differentiating criterion. The content of other fatty acids in Salmonella is similar to that in Escherichia and Shigella. These data confirm that the genera Escherichia, Shigella and Salmonella are phylogenetically related, the relationship between Escherichia and Shigella being more close.

Chromatography, Gas↗
PubMed1982

[Heterogeneity of the fatty acid composition of bacteria in the genera Klebsiella, Enterobacter, Hafnia and Serratia].

The analysis of the chromatograms of methyl esters of fatty acids in bacterial strains of the tribe Klebsielleae showed the heterogeneity of the fatty acid composition of bacteria belonging to the genera Klebsiella, Enterobacter, Hafnia and Serratia. The bacteria of this tribe could be subdivided into 5 provisory groups in accordance with the composition and profile of their fatty acids. Group I comprised K. pneumoniae K1, K. ozaenae and K. rhinoscleromatis strains forming a separate group characterized by the absence of cyclopropane fatty acids; group II comprised K. pneumoniae strains of other capsular serovars (K2, K8, K11, K13, K41, K47), as well as K. aerogenes and K. oxytoca strains. The fatty acid composition of the strains of group III, comprising E. aerogenes and E. cloacae, was similar to that of E. coli O1; only among the fatty acids of these bacteria acid C20:0 could be detected. H. alvei strains were included into group IV; their fatty acid profiles were similar to those of the genus Enterobacter, but had a higher content of acids C15:0 and C18:0. S. marcescens strains were similar to the strains of group II in their fatty acid composition, but considerably differed from the latter by a higher content of hexadecanoate (C16:0) and a lower level of C18:1; for this reason they were regarded as provisory group V.

Chromatography, Gas↗
PubMed1980

[Differentiation between B. pertussis and B. parapertussis according to their fatty acid composition].

The fatty acid composition of 3 B. pertussis strains and 2 B. parapertussis strains grown on casein-carbon agar (CCA) with 8% of sheep blood added and without blood, as well as B. parapertussis strain grown on beef-extract agar (BEA) has been studied by gas chromatography. The fatty acid profiles characteristic of B. pertussis and B. parapertussis were greatly different, as B. parapertussis has a considerable amount of methylene-hexadenocanoic acid, while containing less hexadecenoic and octadecanoic acids and more tetradecanoic acid. The fatty acid composition of 2- to 5-day Bordetella cultures grown on CCA with and without blood has no essential differences. Differences in the content of various fatty acids in B. parapertussis grown on CCA and BEA had no essential influence on the fatty acid profile. The specificity of the fatty acid composition of B. pertussis and B. parapertussis allows to use this characteristics for their differentiation.

Bordetella↗
PubMed1979

[Sh. flexneri population heterogeneity according to the cellular fatty acid makeup].

The composition of fatty acids in the cells of 100 clones of Sh. flexneri, strain 9054, isolated from a dysentery patient was studied by the method of gas chromatography. Fatty acid composition in 75 clones was similar to the fatty acid composition of the initial culture: 25 clones differed to a variable degree from the prevailing group of clones in the content of unsaturated and cyclopropane fatty acids and, to a considerably lesser extent, in the content of palmitic acid and a number of other saturated fatty acids. After the change of the culture medium (agar and broth) the differences in the fatty acid composition of the clones could still be observed. The data obtained in this study indicate the heterogeneity of Shigella population in the composition of cellular fatty acids.

Chromatography, Gas↗
PubMed1977

[Relationship between the culture medium and the fatty acid composition of diphtheria and non-pathogenic corynebacteria].

The gasochromatic method was applied to the study of the cellular fatty acids composition in diphtheria and nonpathogenic corynebacteria (diphtheroids and psendo diptheria bacillus). Marked differences in the content of unsaturated fatty acids were revealed in them. Thus, palmito leic acid served the preponderant unsaturated fatty acid in Corynebacteria diphtheriae, and unsaturated fatty acids with 18 carbon atoms (octadeconoic and linoleic)--in nonpathogenic corynebacteria. The mentioned changes permit use this sign as differential. When grown on Loeffler's medium all the corynebacteria under study had a similar fatty acid composition characterized by the prevalence of unsaturated fatty acids with 18 carbon atoms. On the basis of studying the fatty acid spectrum of the nutrient media used it is supposed that one of the factors determining the revealed dependence of the corynebacterial fatty acid composition on the culture medium was the fatty acid composition of the latter.

Adult↗
PubMed1977

[Fatty acid makeup of various Brucella species and its relationship to the culture medium].

Gas chromatographic method was applied to the study of the fatty acid composition (in Br. melitensis, Br. abortus, Br. suis, and Br. ovis strains. Fatty acid composition was similar in the mentioned brucellae species, except Br. suis No. 1330 significantly differing by this sign. Methyleneoctadecanoic acid content was considerably elevated, and that of octadecenoic -- reduced in brucellae grown on liver agar with the addition of serum and on meat-peptone agar in comparison with brucellae grown on liver agar; apparently this represents one of the mechanisms of the microorganism adaptation to the less favourable conditions of the nutrient medium. Passage of Br. ovis strain through the guinea pig organism led to the appearance of brucellae forming two types of colonies when grown on liver agar with the addition of serum. The fatty acid composition of brucellae forming small transparent colonies was the same as that of the initial culture with the prevalence of methyleneoctadecanoic acid; as to brucellae with larger colonies with irregular margin and nontransparent centre of the colony--octadecenoic acid prevailed in their fatty acid composition, i.e. their composition was similar to such in brucellae of the melitensis and abortus species grown on liver agar.

Brucella↗