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Biomedical subjects

Z P Huang

Publications and source records attributed to Z P Huang.

13 recordsLinked to original sources
PubMed2004

Uterine expression of implantation serine proteinase 2 during the implantation period and in vivo inhibitory effect of its antibody on embryo implantation in mice.

The aim of the present study was to examine the uterine expression pattern of implantation serine proteinase 2 (ISP2) protein during early pregnancy in mice and the effects of anti-ISP2 antibody on embryo implantation. Expression of ISP2 protein was found to be specifically up-regulated in mouse uterine endometrial glands following the initiation of embryo implantation. Similarly, ISP2 protein expression was observed during pseudopregnancy, indicating that its expression is not embryo dependent. In other experiments, rabbit anti-ISP2 IgG was infused into the mouse uterine lumen on Day 3 or 4 of pregnancy to examine its effects on embryo implantation, whereas vehicle (saline) or unspecific rabbit IgG served as controls. The mean number of implanted embryos from anti-ISP2-IgG-treated mice was significantly lower than that from control mice. These results suggest that ISP2 may play an important role during embryo implantation.

Animals↗
PubMed1999

[Induced-differentiation and cytotoxicity of isoverbascoside on HL-60 cells].

HL-60 cells were treated by isoverbascoside with different time and different concentrations in vitro. The differentiation of HL-60 cells was evaluated by light and electron microscopy to observe morphological changes, by chemiluminence to detect phagocytosis and by tumorigenesis in nude mice to determine malignancy. The cytotoxical effect of isoverbascoside on HL-60 cells was examined by trypan blue excluding staining and electron microscopy. The influence of isoverbascoside on cell cycle was measured by flow cytometry. Granular differentiation of HL-60 cells was induced by isoverbascoside at 20-25 mumol/L within 1-3 days as the results of morphological changes, enhancement of phagocytosis and decreasing of tumorigenesis. Strong cytotoxicity was evidenced in HL-60 cells treated by isoverbascoside at 30-35 mumol/L. HL-60 cells treated by isoverbascoside at 20 mumol/L were delayed at G1 phase at 12 hours and G2/M phase at 72 hours.

Animals↗
PubMed1994

[Effect of radix Angelicae sinensis on serum gastrin levels in patients with cirrhosis].

Acute and chronic effect of Radix Angelicae Sinensis (RAS) on serum gastrin levels in patients with cirrhosis were investigated. The results showed that after intravenous perfusion of RAS, serum gastrin levels of inferior vana cava, hepatic and peripheral veins were significantly decreased. After long-term administration of the agent, the level fell nearly to that of control subjects. It is suggested that the effect of reducing serum gastrin level by RAS may improve portal hemodynamics and be beneficial for portal hypertensive gastroduodenal mucosal lesions in cirrhosis.

Adult↗
PubMed1992

[An experimental study of gentamycin delivery into cornea and aqueous humor from the collagen corneal shield].

Collagen corneal shields immersed in 13.33 mg/ml gentamycin solution for 5 minutes were placed in rabbit eyes and the gentamycin concentrations in the cornea and aqueous humor were determined with radioimmunoassay. The results were compared with those achieved by soft contact lens, subconjunctival injection, and frequent instillations. It was found that (1) 1 and 3 hours after application, the collagen corneal shield produced significantly higher concentration of gentamycin in the aqueous than did the other methods, and in the cornea than did the contact lens or instillations; and (2) 6 hours after application, the antibiotic level in the cornea dropped lower than that by subconjunctival injection, but still 15-30 times higher than the bactericidal concentration.

Administration, Topical↗
PubMed1990

[A quantitative diagnosis of chronic gastritis].

To explore a definite diagnostic criterion of chronic gastritis, we undertook a quantitative study of the gastric biopsy specimens taken from 131 subjects. Seven stages were classified from normal gastric mucosa to mild, moderate and severe superficial and atrophic gastritis. The results shown the number of inflammatory cell was much greater in the mild superficial gastritis (128 +/- 84.37/HP) than in normal gastric mucosa (22 +/- 10.54HP, P less than 0.001). In these stages, the numbers of pyloric and fundic gland progressively decreased, the size of the gastric gland gradually enlarged and the percentage of intestinal metaplasia by degrees increased. According to these, we establish the quantitative diagnostic criterion of chronic gastritis.

Adolescent↗
PubMed1989

[Relationship between urinary hydroxyproline creatine ratio and growth rates in boys of age 13 to 16].

A total number of 241 boys aged 13 to 16 were studied longitudinally for 8 months from April 1986 to December 1986. The linear correlation analysis showed that urinary HP/Cr was highly correlated with height growth rate (r = 0.83) and moderately with weight growth rate (r = 0.63). The multiple correlation coefficients between urinary HP/Cr and height growth rates were much bigger than those between urinary HP/Cr and weight growth rates, only the former were statistically significant. It is suggested that the urinary HP/Cr is mainly correlated with height growth rate. The multiple regression analysis was employed with urinary HP/Cr, age as independent variables and height growth rate as dependent variable. The equation is very significant, 95% confidence limit is y +/- 1.38%. It is suggested that the prediction of height growth rate is rather precise, so urinary HP/Cr is a valuable index in the evaluation of growth of children.

Adolescent↗
PubMed1984

Nuclear pore complex in spermatogenesis of Beijing domestic duck. Electron microscope observation of freeze-etching.

The nuclear pore complex in spermatogenesis of Beijing domestic duck has been studied by means of electron microscopic observation of freeze-etching. The structure of the pore observed proves to be in accord with the model of the nuclear pore complex proposed respectively by Franke (1970) and Roberts et al. (1970). But its central granule may be present (1-3 granules) or absent. The granules are considered to be material in transit. The size and frequency of the pore vary with different types of reproductive cells, which is believed to be related to the cells' function. The pores concentrate on the side of nucleus near Golgi zone in primary spermatocyte and near the proacrosome of spermatid, and this shows the functional variety of parts in the nucleus.

Animals↗
PubMedDate not supplied

An enzymic digestion and solid-phase extraction procedure for the screening for acidic, neutral, and basic drugs in liver using gas chromatography for analysis.

Analysis of liver specimens is an important issue in forensic toxicology, but suitable workup and extraction methods for general screening purposes have been lacking until now. A workup and extraction scheme based on a recently developed procedure for the screening of biological fluids was developed that can be used for the screening of acidic, neutral, and basic drugs in liver. This method uses a single solid-phase extraction (SPE) column and gas chromatography-flame ionization detection (GC-FID) for the final analysis. First, the homogenized liver sample is sonicated and centrifuged; the resulting supernatant is applied to the SPE column. Elution of acidic, neutral, and some weakly basic drugs is then performed with acetone-chloroform and analyzed by GC-FID. Next, the pellet of tissue material obtained from the centrifugation is enzymically digested by subtilisin Carlsberg. This frees the drugs bound to the liver tissue. The resulting clear liquid is brought to the reconditioned SPE column. A wash step is introduced to remove acidic and neutral interferences and the basic drugs can then be eluted with ammoniated ethyl acetate. Using 100-mg wet liver samples spiked with 2 micrograms of amounts of various drugs, recoveries were 70-102% with relative standard deviations less than 9%. The resulting GC-FID chromatograms were virtually free of endogenous interferences. GC-nitrogen-phosphorous detection detected smaller amounts of nitrogen-containing drugs, again without endogenous interferences. With the SPE columns currently used, which contain a bed mass of 130 mg, the liver samples should be smaller than 200 mg because the endogenous compounds obtained after the digestion of the tissue will overload the column, which results in a lower recovery of the drugs of interest. Drugs that decompose under the digestion conditions (pH 10.5 at 60 degrees C for 1 h) may be lost in the present procedure. This phenomenon is being investigated further.

Flame Ionization↗