Search PubMed⌕ Search

Biomedical subjects

Z OVARY

Publications and source records attributed to Z OVARY.

At least 19 recordsLinked to original sources
PubMed1963

PROPERTIES OF GUINEA PIG 7S ANTIBODIES. V. INHIBITION BY GUINEA PIG GAMMA-2 ANTIBODIES OF PASSIVE IMMUNE LYSIS PROVOKED BY GAMMA-2 ANTIBODIES.

Passive immune lysis of antigen-coated erythrocytes provoked in vitro by guinea pig gamma(2) antihapten antibodies in the presence of complement, is inhibited by guinea pig gamma(1) antibodies directed against the same specificity. This inhibition of the lytic effect of complement-fixing gamma(2) antibodies is presumably due to the competition for antigen by non-lytic gamma(1) antibodies.

Animals↗
PubMed1963

STRUCTURE AND SPECIFICITY OF GUINEA PIG 7S ANTIBODIES.

Additional evidence has been obtained to show that different guinea pig anti-hapten antibodies differ in the structure of their L polypeptide chains. Antibodies from animals immunized with the same hapten conjugated to different carrier proteins gave similar starch gel electrophoretic patterns after dissociation of their chains. In a study of fine differences of specificity, cross-reacting antibodies were found to have some L chains with the same electrophoretic mobility. The multiplicity of L chain bands found in the characteristic starch gel electrophoretic patterns of dissociated anti-DNP antibodies was shown to be a reflection of the heterogeneity of antibodies of slightly different specificities. Reduction and alkylation of the active fragment produced by digestion of antibodies with papain yielded starch gel electrophoretic bands corresponding in mobility to L chains. The results are consistent with the notion that L chains are involved in the acquisition of immunologic specificity.

Animals↗
PubMed1963

Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies.

Guinea pigs hyperimmunized with single protein antigens or hapten conjugates emulsified in complete adjuvants produced two types of precipitating antibodies with different electrophoretic mobilities. "Slow" migrating antibody generally appeared earlier and "fast" migrating antibody later in the course of immunization. Animals initially immunized by the intraperitoneal route with hapten conjugates without adjuvants produced primarily fast migrating antibody. Purified guinea pig antibodies were also separable into slow and fast migrating components by electrophoresis in supporting media. Using suitable antisera prepared in rabbits hyperimmunized with guinea pig serum, it was demonstrated that slow and fast antibodies have both common and distinct antigenic determinants. Analytical ultracentrifugation disclosed that both antibodies have sedimentation coefficients of approximately 7S. These antibodies have been designated guinea pig 7Sgamma(1) and 7Sgamma(2).

Animals↗
PubMed1963

Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis.

Guinea pig 7Sgamma(1) antibodies were demonstrated to mediate passive systemic or cutaneous anaphylaxis; guinea pig 7Sgamma(2) antibodies were unable to mediate these reactions. Gamma-2 antibodies specifically inhibited passive cutaneous anaphylactic reactions provoked by gamma-1 antibodies by competing for antigen. However, gamma-2 antibodies were unable to inhibit passive cutaneous sensitization of guinea pigs by a heterologous antibody system. Guinea pig 7Sgamma(2) antibodies appear to lack receptors for fixation to guinea pig tissues and do not compete with sensitizing antibody for receptor sites.

Anaphylaxis↗
PubMed1963

Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis.

Guinea pig 7Sgamma(2) antibodies were demonstrated to fix complement in the presence of antigen and to sensitize antigen-coated, tanned erythrocytes for lysis in the presence of complement; guinea pig 7Sgamma(1) antibodies did not participate in these reactions. Gamma-2 antibodies were more efficient in provoking hemorrhagic necrosis in reverse passive Arthus reactions than equal amounts of non-complement-fixing gamma-1 antibodies. Unlike anaphylaxis in the guinea pig, both guinea pig 7Sgamma(1) and 7Sgamma(2) antibodies provoked passive cutaneous anaphylactic reactions in the rat. Efficient hemolytic activity attributable to 7S guinea pig anti-sheep erythrocyte antibodies migrated faster than the peak of complement-fixing activity, but slower than the peak of PCA activity in starch block electrophoresis. It is uncertain whether this activity is a function of a third type of antibody produced in response to the particulate property of the antigen or whether it is due to the antigenic heterogeneity of the erythrocyte cell membrane.

Anaphylaxis↗
PubMed1963

Passive cutaneous anaphylaxis with antibody fragments.

Pepsin-digested rabbit antibody (5S) provoked reverse passive cutaneous anaphylaxis in the guinea pig, but was somewhat less effective, mole for mole, than the native antibody. Fixation of guinea pig complement by the pepsin-digested antibody could not be demonstrated either in vitro or in vivo. Splitting of the 5S fragment into two monovalent fragments (3.5S) markedly reduced the capacity to provoke reverse passive cutaneous anaphylaxis.

Anaphylaxis↗