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Biomedical subjects

Z Nagy

Publications and source records attributed to Z Nagy.

At least 235 records · Page 13Linked to original sources
PubMed1982

Cytotoxic material released from Staphylococcus epidermidis. I. Effects on [3H]thymidine incorporation of human lymphocytes.

Incorporation of [3H]thymidine ([3H]TdR) into tonsil lymphocytes was inhibited by native Staphylococcus epidermidis while Staphylococcus aureus Cowan I caused stimulation. The inhibitory effect of S. epidermidis was abolished by formalin treatment but not by heat killing. A toxic agent was released from S. epidermidis on gentle water extraction without lysing the bacteria. The extract contained protein and other UV-absorbing material, but did not exhibit haemolytic, lysozyme, catalase or protease activity. The heat-resistant, formalin-sensitive inhibitor present in the aqueous extract of S. epidermidis inhibited [3H]TdR incorporation of lymphocytes in a dose-dependent manner and decreased the viability of lymphocytes.

Cytotoxins↗
PubMed1982

[Percutaneous kidney calculus removal].

The authors describe the method of percutaneously removing renal calculi. The application of the percutaneous method was made possible in their case by the fact that, following pyelotomy, a nephrostomy had been previously inserted into the system of renal cavities of the right side in a polytraumatized patient. Two recidive calculi formed in the immobilized patient; after the patient's mobilization the authors removed these through the opening of the drain with lithotomy forceps and with the help of an image intensifier.

Adult↗
PubMed1982

Cytotoxic material released from Staphylococcus epidermidis. II. Fractionation and some effects of the fractions on lymphocytes and hepatocytes.

Cytotoxic substance(s) of about 4 X 10(3) molecular weight, containing 9.5% peptide and 73% carbohydrate was released from Staphylococcus epidermidis in phosphate buffered saline. The material was soluble in ethanol and was heat-resistant. It blocked amino acid uptake and E-rosette formation of human tonsillar and blood lymphocytes. In isolated mouse hepatocytes the toxin inhibited protein synthesis, but only in the presence of calcium ions. The results suggest that eukaryotic cell membranes are damaged by the coccal agent.

Animals↗
PubMed1982

[Epidermoid cyst of the testis].

After a brief review of the literature on the epidermoid cyst of the testicle, the aspects of the disease are discussed. It stressed that castration is an unavoidable part of the treatment of this type of cyst.

Adult↗
PubMed1981

Endothelial surface charge: blood-brain barrier opening to horseradish peroxidase induced by the polycation protamin sulfate.

Perfusion of rat brain with the polycation protamin sulfate caused a decrease in the stainability of luminal surface of cerebral endothelium to positively charged colloidal iron. This change reflecting a decrease in the anionic sites of the endothelial plasma membrane was accompanied by opening of the blood-brain barrier to horseradish peroxidase. The polyanion heparin reversed colloidal iron binding but failed to prevent permeability alteration of the cerebral endothelium.

Animals↗
PubMed1981

Study of infective (secondary) renal calculus formation in vitro.

The factors of infective calculus formation and the conditions favoring their prevention were studied by examining in vitro the role of the most common representative of urease-producing bacteria, Proteus mirabilis. The stream of Proteus-containing urine was found to form deposits of magnesium ammonium phosphate, carbonate apatite and ammonium-urate on the glass surfaces. Application either of mandelic acid or of Gentamycin was found to reduce the production of these deposits, and their joint application proved fully preventive to their formation. Gentamycin applied in saline acidified with mandelic acid was capable of dissolving previously formed crystals. A medication of this composition is advocated for preventive use after surgery for staghorn calculi. The necessity for continuing peroral medication and specific antibacterial therapy for months is emphasized.

Crystallization↗
PubMed1980

Experimental study of marginal wedge resection and its practical application in the surgical therapy of staghorn calculi.

A surgical procedure for staghorn calculi, which are inaccessible to conservative therapy, is described. It not only provides for complete elimination of the calculus but may also contribute to the prevention of recurrences. The technique of marginal wedge excision of the renal parenchyma, developed in animal studies, is described in detail and 11 surgical cases are reported. Removal of staghorn calculi was successful all throughout, and renal function remained unaffected by the intervention in all of the cases. Recurrences were confined to a single case. The procedure is safe, it involves no greater surgical strain than does marginal nephrotomy, but its short-term metaphylactic results seem more promising. Assessment of the long-term results awaits further observations.

Adult↗
PubMed1980

The adherence of Staphylococcus epidermidis to human tonsil lymphocytes.

Attachment of various bacteria to human, peripheral blood and tonsil lymphocytes was investigated in vitro. About 20% of tonsil lymphocytes bound Staphylococcus epidermidis, whereas the binding of other strains was negligible. The influence on cytoadherence of human serum, immunoglobulins (human IgM, IgG, IgA, as well as their respective anti-Ig's), and carbohydrates (mono and polysaccharides) was measured. It was found that heterogenous surface structures of the lymphocytes participate in the attachment.

B-Lymphocytes↗
PubMed1979

Opening of tight junctions in cerebral endothelium. I. Effect of hyperosmolar mannitol infused through the internal carotid artery.

Infusion of 1.8 M mannitol solution into the internal carotid artery of Wistar rats allows horseradish peroxidase (HRP) to cross cerebral vascular endothelium via intercellular pathways. This was made evident by density gradients of HRP observed in consecutive tight junctional compartments following in vivo administration, and by the passage of colloidal lanthanum through junctional membrane appositions of glutaraldehyde fixed cerebral endothelium. Three hours after mannitol infusion, small non-capillary vascular segments were still focally permeable to HRP. Twenty-four hours after the osmotic insult, the tracer was absent in the affected hemisphere. Water and electrolyte assays indicated absence of brain edema during the period of observation of blood-brain barrier opening.

Animals↗
PubMed1979

Opening of tight junctions in cerebral endothelium. II. Effect of pressure-pulse induced acute arterial hypertension.

Acute arterial hypertension was produced in male Wistar rats by pressure pulse through the right internal carotid artery. The pressure pulse was induced by infusion of physiological saline as a bolus, at a rate of 0.63 ml per second by syringe pump. Evans blue (Eb) was used to visualize the areas of blood-brain barrier opening. Intravenously injected horseradish peroxidase (HRP) was used to study the ultrastructural basis of permeability changes in cerebral endothelium. Eb outlined circumscribed areas of blood-brain barrier opening. HRP extravasation was found mainly around small arteries. The capillary network was affected to a much lesser extent. Electron microscopy showed that HRP crossed the endothelial cell layer by intercellular routes. Glutaraldehyde fixed brain samples permeated with colloidal lanthanum supported these observations.

Animals↗
PubMed1979

Blood-brain barrier opening to horseradish peroxidase in acute arterial hypertension.

Acute arterial hypertension was induced in male Wistar rats using two experimental techniques: (1) i.v. injection of Aramine and (2) infusion of physiological saline as a bolus via internal carotid artery. Horseradish peroxidase (HRP) was injected i.v. prior to both experimental procedures and subsequently localized in the brain by light and electron microscopy. In the saline infusion (pressure pulse) model, colloidal lanthanum was also applied as a diffusion tracer following fixation of the cerebral endothelium. In the Aramine model, extravsation of HRP correlated with abrupt elevation of blood pressure. In the pressure pulse model HRP extravasation was consistently visualized in the affected hemisphere. Electron microscopy showed consistent labeling of plasmalemmal vesicles by HRP in segments of cerebral endothelium. However, HRP was also clearly visualized in junctional pools suggesting focal opening of endothelial tight junctions as a pathway for extravasation of this tracer in both hypertensive models. Colloidal lanthanum not transported by plasmalemmal vesicles across endothelium after fixation of the brain also bypassed consecutive membrane appositions of endothelial tight junctions indicating existance of interendothelial pathways to macromolecules in acute arterial hypertension.

Acute Disease↗