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Biomedical subjects

Z Marhoul

Publications and source records attributed to Z Marhoul.

At least 19 recordsLinked to original sources
PubMed1986

Some diagnostic, biologic and morphologic characteristics of Francisella tularensis strains isolated from the ticks Ixodes ricinus (L.) in the Prague agglomeration.

Four infectious agents were isolated from the ticks Ixodes ricinus (L.) collected in the recreational area and park-forest of Prague. On the basis of cultivation, staining, biochemical, serologic properties, pathogenicity for animals and histological tests they were identified as Francisella tularensis with the following features: they are short, gram-negative rods of approximate dimensions of 0.3 X 0.8 micron, growing in enriched media after 3-4 day incubation at 37 degrees C. They form small circular, at first transparent, later greyish turbid colonies with regular rims. They are little active biochemically. They are susceptible to streptomycin and some broad spectrum antibiotics. They react positively with tularemic serum, but in lower titres than those in which this serum reacts with standard antigen. The microbes are highly pathogenic for mice, guinea-pigs, young rats, in which a massive bacteriemia occurs before death, but they do not kill rabbits. They multiply well in chick embryo, but do not grow in cell or tissue cultures. The most important histologic changes were observed in liver and spleen of mice. No pathologic changes were found in brain, lungs, heart, kidneys. Necroses were found in liver and in their marginal zones the microbes were present. Conspicuous were changes in numerous hepatocytes which became enlarged due to microbial multiplication and finally transformed into "sacs" packed with microbes. Histological and electronoptical examination showed that these are intracellular parasites fringed with a light lytic zone. Discussed is the problem to what extent the properties of the isolated strains are typical of F. tularensis as well as the importance of their detection from the aspect of epidemiology and differential diagnostics.

Animals↗
PubMed1984

Ultrastructure of Taenia saginata oncospheres cultivated in artificial media.

Oncospheres of Taenia saginata cultivated in Leibovitz's medium on a cell monolayer from calf kidney developed up to 10th day of in vitro cultivation. Fine and often branched microvilli were observed on their surface. On day 5 of cultivation, the muscle system of hooks turned to a granular matter and on day 7 neither hooks nor their muscle system were found. The tegumental cytoplasm was not formed in the period of 7-60 days of cultivation. On days 7 and 10 of cultivation, a contact of oncospheres with the cells of monolayer was visible. On day 10 of cultivation, an extrusion of a portion of cytoplasm of surface cells filled with minute vesicles occurred. The bonds among cells, particularly in the centre of the oncosphere, were loosened in a 30-day-old culture and the cells were interconnected by long cytoplasmic bridges. Degenerative changes occurred on about day 40 of cultivation. The oncospheres cultivated in pure Leibovitz's medium lost the microvilli on their surface and the original tegumental cytoplasm turned to a fibrous layer already on day 5 of cultivation. Dystrophic changes in cells occurred from day 7 of cultivation and gradually increased.

Aging↗
PubMed1978

Rapid diagnosis of tick - borne encephalitis by immunofluorescence assay of specific serum IgM antibodies.

The indirect IF technique, using suspensions of TBE virus infected and uninfected PS cells as antigen-containing substrate, furnishes a rapid and practical test making possible the detection of specific IgM class serum antibodies in the initial stage of clinically manifest TBE. It enables early confirmation of diagnosis already in the acute phase of the disease and thus it can be instrumental in differential diagnosis and rational therapy, e.g., the administration of specific hyperimmune gamma-globulin.

Antibodies, Viral↗
PubMed1976

Influence of temperatures corresponding to those of the host animals on Tahyna virus.

The influence of temperature of 36.5 and 39.2 degrees C on uncloned low passage Tahyna virus in chick embryo cell cultures was studied in the course of 10 passages. At a high multiplicity of infection, the virus titres showed a considerable variability, especially at 39.2 degrees C. The cytopathic effect (CPE) apparent at both temperatures started later and was of lower intensity at 39.2 degrees C than at 36.5 degrees C. The temperature of 39.2 degrees C intensified the thermostable character of the virus. Changes in plaque size and virulence were connected mainly with the cultivation substrate of the virus.

Animals↗
PubMed1976

Influence of temperature corresponding to that of the vector on Tahyna virus.

The behaviour of uncloned, low-passage Tahyna virus in an Aedes albopictus (AA) cell line at 28 and 20 degrees C was studied in the course of 10 passages. The virus multiplied at both temperatures without any apparent effect on the host cell. At 28 and 20 degrees C reduction of plaque size, decrease of peripheral virulence and weakening of thermostability were observed. Differences between both temperatures were only in the intensity of these changes.

Aedes↗
PubMed1976

Cultivation of Lednice (Yaba 1) virus in goose, duck, and chick embryo cells.

Lednice (Yaba 1) virus was found to multiply in primary cultures of goose, duck and chick embryo cells. The size of inoculum and age of embryos used for preparation of primary cultures was decisive for the amount of multiplied virus. One strain (110) of Lednice virus showed reduced ability to induce a cytopathic effect in the first passage in chick embryo cells. Virus multiplication in all three kinds of cells was confirmed by immunofluorescence.

Animals↗