Search PubMed⌕ Search

Biomedical subjects

Z Magić

Publications and source records attributed to Z Magić.

9 recordsLinked to original sources
PubMed1999

Early inflammatory cytokine and acute phase protein response under the stress of thermal injury in rats.

The acute inflammatory response associated with thermal injury was examined in rats. The appearance of mediators of inflammation in the systemic circulation, including cytokines interleukin-1 (IL-1), tumor necrosis factor (TNF) and interleukin-6 (IL-6) and acute phase proteins were assessed during initial 72 h following thermal injury. Increased levels of activity were noted for all three cytokines, but with a different time-course. While serum IL-1 activity was elevated throughout the 3-day period of observation, the levels of serum TNF activity were enhanced after 12 h and on days 1 and 3 following scalding injury. The values of IL-6 were already increased one hour after thermal injury and increased progressively up to day 1 following scalding. Alpha2-macroglobulin and haptoglobin levels were increased 12 h after thermal injury, rising further on days 1 and 3. Positive correlation was found between the time-course of increased serum IL-6 activity and alpha2-macroglobulin, as well as between TNF and haptoglobin in the serum.

Acute-Phase Reaction↗
PubMed1997

Re-establishment of homeostasis and the acute-phase proteins.

The stimulation of transcription of acute-phase protein (APP) genes in the liver is incorporated in the complex interchange of cytokines, growth factors and glucocorticoid hormones that are released during the systemic defence reaction in response to trauma. Through the broad spectrum of their activities, this heterogeneous group of circulating proteins assists the injured organism in restoring homeostasis by assuming a protective role. APPs accomplish this by inactivating vasoactive, proteolytic and cytotoxic molecules liberated from damaged tissues and accumulating phagocytic cells, and by participating in a feedback control mechanism that prevents an overload by the organisms' immune response. APP synthesis represents a non-specific response of the liver, in so much as different types of trauma elicit the production of the same proteins. However, data obtained from different laboratory models and clinical observations revealed a certain relationship between the severity and type of trauma and the magnitude of activation of APP gene expression. The observed variations of the overall pattern of APP synthesis point to the existence of different interplays between humoral and cellular mediators capable of adjusting the production of individual proteins to suit different traumas. Hence, changes in the serum concentrations of some APPs have been shown to be useful in monitoring complications such as infection or sepsis after surgery or trauma, and predicting the clinical course of malignant and other diseases. Of the APPs studied in humans, information obtained on CRP and SAA has in particular proved to be a useful indicator of the progression of different pathological states.

Acute-Phase Proteins↗
PubMed1995

Ionizing radiation-induced expression of the genes associated with the acute response to injury in the rat.

Total-body irradiation of rats with doses ranging from an LD10/30 to an LD100/30 induced a dose-dependent increase in the concentration of serum protein associated with the acute response to the irradiation. However, this increase was reached at a later time and was not as pronounced as described previously during the typical acute phase of the response found experimentally (A. Koj, in Structure and Function of Plasma Proteins, Vol. 1, pp. 73-131, Plenum Press, London, 1974). The greatest increase in the serum concentrations of acute-phase proteins was found from the third to the seventh days postirradiation. At these times, the serum concentrations of alpha 2-macroglobulin, haptoglobin, fibrinogen and cysteine protease inhibitor were raised from two- to fivefold, whereas alpha 1-acid glycoprotein was increased sixfold. Incorporation of [35S]methionine into total serum and acute-phase proteins indicated that the increase in the concentration of the acute-phase proteins was preceded by their de novo synthesis in the liver. The results that were obtained by dot-blot analysis showed that the basic course of change in the relative mRNA concentrations in the liver for the acute-phase proteins examined correlated with the changes in their protein concentrations in the serum; only the relative increase in the concentration of alpha 1-acid glycoprotein mRNA was significantly lower than the increase in proteins in the serum, suggesting that a fraction of the serum alpha 1-acid glycoprotein had an extrahepatic origin. On the basis of these results we concluded that total-body irradiation increased the expression of acute-phase protein genes.

Acute-Phase Proteins↗
PubMed1986

Effect of non-lethal scalding on the amount of DNA and RNA in rat liver.

The quantities of DNA and RNA were measured in rat liver homogenate, supernatant and nuclei after scalding. Maximal increases of nuclear DNA and RNA were found 12 h after thermal injury. The RNA content in the rat liver supernatant and homogenate reached its maximum at 24 and 48 h, respectively. The results obtained in this study show that the thermal injury resulted in an activation of the genetic apparatus in rat liver cells. These findings correspond well with a significant role of the liver in post-traumatic metabolism.

Animals↗
PubMedDate not supplied

Variability of haptoglobin and alpha 2-macroglobulin in Albino Oxford and Dark Augustin inbred strains of rats before and after trauma.

Increased synthesis of acute-phase proteins (APP) in the liver represents the most prominent aspect of acute phase reaction (APR). Haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2-M) are among the most important APP in rats. In this study AO (Albino Oxford) and DA (Dark Augustin) inbred strains of rats, with different resistance to trauma, were used to compare concentrations of Hp, alpha 2-M, IL-1, IL-6 and TNF, before and after tourniquet trauma. Cytokines IL-1, IL-6 and TNF have an important role in APP gene expression. Our results showed that before trauma, more resistant AO rats had higher concentrations of Hp and alpha 2-M compared to DA rats. The influence of genetic elements of these differences is significant. Tourniquet trauma caused an early increase of IL-1, IL-6 and TNF activities in the serum which preceded the increase in the Hp and alpha 2-M concentrations but differences between strains were not found.

Animals↗
PubMedDate not supplied

[Acute phase proteins].

Explore the source record for details and available documents.

Acute-Phase Proteins↗