[Comparison of heterologous adult filarial antigens in detecting serum antibodies in Bancroftian filariasis patients by indirect fluorescent antibody test].
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Animals↗
Biomedical subjects
Z M Feng
Publications and source records attributed to Z M Feng.
A clone of hepatitis B virus (subtype adr) DNA with a new HindIII site.
We have cloned the HBV genome subtype adr through its HindIII site into plasmid pBR322. Twelve recombinant plasmids each with an insert of the HBV genome were obtained. The restriction map of one of the recombinants, plasmid pADR-H1, was analyzed. The location of the sites for BamHI, BglI, BglII, SstII, XbaI and XhoI in pADR-H1 were found to be the same as that in pADR-1, but the HindIII site of pADR-H1 is distinctly different from that of pADR-1. The BamHI-HindIII fragment is 316 bp long in the genome of pADR-1. However, it is only 82 bp in pADR-H1. No deletion of sequence between BamHI and HindIII sites has been found in the HBV genome of pADR-H1. The sequencing data around the HindIII site of pADR-H1 in both pADR-H1 and pADR-1 showed that there is a stretch of AAGTTT in pADR-1 compared to an AAGCTT in pADR-H1. In addition, other differences were found. There are three sites for AvaI, four for HincII, one for HpaI and none for SphI in the genome of pADR-H1 compared to four sites for AvaI, three for HincII, one for SphI and none for HpaI in pADR-1. The biological significance of base pair mutation in HBV DNA and the possibility of the existence of HindIII site in the genome of subtypes ayw and adw were discussed.
Base Sequence↗
[The effects of artemether on serum IgG and spleen weight in mice].
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Animals↗
Cloning and restriction mapping of human HBV genome serotype adr.
A Bam HI cleaved 3.2 Kb fragment from the HBV adr genome was cloned in E. coli using pBR322 as vector. Sixteen restriction sites from the action of Bam HI, Hind III, Bgl I, Bgl II, Ava I, Hinc II, Sph I, Xba I, Xho I and Sst II were determined and mapped. No cleavage sites were found for Eco RI, Pst I, Sma I, Hpa I, Kpn I, Pvu II and Sst I. The restriction map for HBV adr is significantly different from those reported for the subtypes adw, ayw and adyw.
Cloning, Molecular↗