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Biomedical subjects

Z Lodin

Publications and source records attributed to Z Lodin.

At least 55 records · Page 3Linked to original sources
PubMed1978

Nuclear pole complexes in cells of developing mouse cerebral cortex.

The nuclear pore complexes of cells of the superficial layers of the cerebral cortex of mice were studied by freezeetch technique from the 18--20th embryonic to 180th postnatal day. The nuclear membrane was found to be randomly penetrated by pore complexes in all age groups studied. The pores have a typical octagonal shape. The density of pores (per/um2) amounted to 7.8, 14.0, 17.0, 18.1 and 14.1 on 18--20th embryonic and 8th, 15th, 50th and 180th postnatal day respectively. The total number of pores per nucleus amounted to 1257, 6582, 5405 and 3384 on the 18--20th embryonic and the 15th, 50th and 180th postnatal day respectively. Thus the total number of pores per nucleus increases 5.2 times from the 18th--20th prenatal to the 15th postnatal day and than slightly decreases toward the 180th postnatal day. The density of pore complexes in adult brain cell nuclei is therefore very high in comparison with other normal somatic and cancer cells.

Age Factors↗
PubMed1977

Transplantation immunity in the brain.

The resistance of animals to allogeneic tumor cells injected into the brain increases with age. The second-set reaction in the brain of rats appears to be equally effective after preimmunization into the brain and preimmunization into the leg. Also, the presence of cytotoxic antibodies is demonstrable after preimmunization into the brain. These findings suggest that both arcs of the transplantation reaction, the afferent and efferent, are involved in tumor-cell allotransplantation in the brain.

Age Factors↗
PubMed1977

Experimental allergic encephalomyelitis. Water, sodium and potassium concentration in spinal cord and hemispheres and their changes after dexamethasone treatment.

Water content, Na+, K+ and Cl- concentration were measured in rats with experimentally produced allergic encephalomyelitis. Increase of water content by 10%, accumulation of Na+ by 13%, of Cl- by 20% and decrease of K+ by 10% was observed in the spinal cord, but no substantial changes were found in the brain tissue. The hydration of spinal cord is accompanied by perivascular infiltrates. Dexamethasone, administered from day 5, after injecting sensitising encephalitogenic basic protein, effects normal levels of water content and Na+, K+ and Cl- concentration.

Animals↗
PubMed1976

Experimental allergic encephalomyelitis.-- The effect of dexamethasone on growth and proliferation in the draining lymph node.

Dexamethasone, when administered from day 0 to day 9 following the sensitizing injection of encephalitogenic protein in complete Freund's adjuvant, suppressed both the characteristic enlargement of the paracortical area of the draining lymph node and the development of clinical and histopathological signs of experimental allergic encephalomyelitis. Division of cells within the node was not inhibited and therefore the effect of dexamethasone appeared to be a consequence of reduced migration of lymphocytes into the node.

Animals↗
PubMed1976

The metabolism of glucose of nerve cells cultivated under different conditions.

1. The consumption of glucose and formation of lactate was studied in medium of long-term cultivated nerve tissue. Fragments of chicken brain embryo dissociated and reaggregated brain cells were cultivated in Rose chambers, Falcon plastic dishes and Erlenmayer flasks. 2. Dissociated cells were cultivated in Petri dishes in media containing 100 mg/100 ml glucose. Consumption of glucose and formation of lactate increases until the 9th day. Glucose is completely exhausted in the medium up to the 2nd week of cultivation. 3. The time curve of both glucose consumption and lactate production is similar in cultures cultivated in Rose chambers, Petri dishes and Falcon plastic dishes. Cultures in Rose chambers utilize glucose at later stages anaerobically, whereas in Petri dishes and Falcon plastic dishes approximately 25% is utilized by aerobic glycolysis. 4. Cells dissociated by trypsinization and sieving are metabolically more active than cells separated mechanically (sieving only). During later stages of cultivation of enzymatically dissociated cells in 100 mg/100 ml glucose, lactate is utilized like a substrate, because of concentration of glucose in the medium is not being sufficient. 5. The concentration of glucose is essential for utilization by the aerobic pathway. In dissociated cells, cultivated in media enriched by 400 mg/100 ml of glucose in Falcon plastic dishes 75% of aerobic glycolysis is found during first 10 days and 50% in later stages. In the same system, cultivated in 100 mg/100 ml of glucose, glucose is exhausted up to the 12th day and lactate is utilized as a substrate. 6. In the close system of cultivation, i.e. in Rose chambers, 50% of glucose is utilized by the aerobic pathway if the medium contains 400 mg/100 ml of glucose. Early cultivation period of dissociated cells in Falcon plastic dishes is slowed, because cells adhere slowly to the plastic ground. 7. Structural development of cultures and differentiation of cells was studied during the cultivation period. Cells cultivated in elevated glucose concentration exhibit signs of better differentiation.

Aerobiosis↗
PubMed1975

An estimate of the number of cells arising by division in mouse cerebral hemispheres from age one to 12 months: an autoradiographic study of DNA synthesis.

Male CBA strain mice aged 1, 2, 4, 6, 8 and 12 months were injected with 5 muCi of 6-H3-thymidine per gm body weight and killed two hours after the injection. Incorporation of the isotope, as a measure of DNA synthesis and cell division, was studied in the cerebral cortex and corpus callosum by light-microscope autoradiography. DNA synthesis was found in a small number of nuclei of non-neuronal cells, both of ecto- and mesodermal origin. The percentage of labeled cells (Labeling Index, or LI) decreased in both regions exponentially with age. On the base of measured LI values, the total number of cells arising from age one to 12 months was calculated. As the reference value the number of cells in one month-old animals was taken. The calculated cell increment in the ectodermal population in the cortex amounted to 19.7-20.6% (average 20.2%) and to 196.0-425.0% (average 247.0%) in the corpus callosum (pairs of figures correspond to values calcualted from LI values measured in sagittal and frontal sections). When all labeled cells (LC) were taken into consideration (of both ecto- and mesodermal origin) the corresponding cell increment ranged from 25.0-30.5% (average 26.8%) in the cortex and from 205.0-454.8% (average 263.0%) in the corpus callosum. The number of all newly arising cells in the whole hemisphere (including both cerebral cortex and corpus calosum) calculated from mean LI values of all LC in sagittal and frontal sections amounted to 52.0%. It is suggested that the observed DNA synthesis and cell division, particularly in the corpus callosum and mesodermal cell groups of both regions studied, correspond mainly to renewal of cell populations. In the cerebral cortex accumulation of some newly arising cells in situ cannot be excluded.

Aging↗
PubMed1975

The influence of l-methionine-d,l-sulfoximine on the labelling of acid soluble fraction and proteins of the brain cortex after intraperitoneal injection of 14c-leucine.

Methionine sulfoximine (MSO) inhibits the labelling of the acid soluble fraction and proteins of the brain cortex after the injection of 14C-leucine. The inhibition takes place even during the early stages after the injection of MSO, when no symptoms of paroxysms are observable. The action of MSO on the labelling of the acid soluble fraction and proteins in the brain cortex is obviously different from that in the kidney and liver. Whereas in the brain tissue MSO markedly influences the labelling of the free amino acid pool, in the kidney and liver it seems primarily affect the protein synthetic mechanisms. Also no decrease in labelling of the plasma acid soluble fraction was found on mice, treated with MSO. Experimental data support the idea, that the changes in the metabolism of proteins in the brain are not connected with the onset of the paroxysmal period.

Animals↗
PubMed1975

Oxygen uptake by suspension of mouse brain cells.

A cell suspension was prepared by sieving mouse brain cortices in an isotonic solution of purified polyvinylpyrrolidone. A large increase on the O2-uptake by the suspension could be obtained with the preparation procedure described. The respiratory rate of suspension, incubated in saline medium, containing 6.2 mM K+, 10 mM pyruvate, 5 mM fumarate and 0.9 mM 5'-AMP, was equal to 68% of the respiratory rate of slices. High K+ concentration (65 mM) stimulated O2-consumption of suspension by 64% (73% in slices).

Adenosine Monophosphate↗