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Biomedical subjects

Z Lin

Publications and source records attributed to Z Lin.

At least 361 records · Page 20Linked to original sources
PubMed1993

Elevated plasma levels of VWF:Ag in hyperthyroidism are mediated through beta-adrenergic receptors.

Thyroid dysfunction influences the levels of Von Willebrand factor (VWF:Ag). Plasma VWF:Ag levels in 35 hyperthyroid patients were significantly elevated, which had a positive correlation with both T3 (Triiodothyronine) and T4 (Thyroxine) levels. A follow-up study of seven hyperthyroid patients undergoing anti-thyroid therapy showed that the elevated VWF:Ag levels returned to normal range with the normalization of thyroid function. On the other hand, after administration of 160 mg/day of propranolol, a specific blocker of beta-adrenergic receptors, to five hyperthyroid patients for twenty-eight days, plasma VWF:Ag levels returned to normal range, while T3, T4 levels remained high without significant alteration. These results demonstrated that the elevation of plasma VWF:Ag by thyroid hormone is mediated through beta-adrenergic receptors, which may be useful to further understand the physiological and pathological roles of thyroid hormone in the regulation of VWF:Ag levels.

Adult↗
PubMed1993

[Forensic toxicological application of TOXI-LAB screening for biological specimens in autopsy cases and emergency cares].

Using the TOXI-LAB drug detection system, emergency toxicological screening was performed in autopsy cases and emergency cares. In 280 autopsy cases (male 182 cases 65%, female 98 cases 35%), drug positive cases were 28 cases of male (15%) and 24 cases of female (24%). The age groups which showed higher rate of drug positive cases were 10s-40s in male (approximately 20%) and 20s in female (67%). In the 238 cases of emergency care (male 129 cases 54%, female 104 cases 44%, unknown 5 cases 2%), drugs were positive in 29 cases of male (22%) and 32 cases of female (30%). The age groups which showed relatively higher rate of drug positive cases were 40s in male (64%), 20s (71%) and 30s (89%) in female. Forty-four different kinds of drugs were detected in TOXI-LAB positive cases, in which the psychotropic drugs and the sedative-hypnotic drugs amounted to approximately 70%. Methamphetamine and amphetamine, which were the main abused drugs showing a socially important problem, were detected in total 15 cases. TOXI-LAB was based on thin-layer chromatography (TLC); however, from the extraction to development, coloration and detection have been accelerated (about 50 min) and simplified. In order to perform the forensic toxicological practice in Japan, it becomes more useful that TOXI-LAB is used in autopsy cases and emergency cares, if the drugs, which have caused poisoning cares in Japan, are added to TOXI-LAB. The present study describes the advantage and problem of TOXI-LAB drug detection system through demonstrating the practical cases of autopsy cases and emergency cares.

Emergencies↗
PubMed1993

[The comparative analysis of the complications of extracapsular cataract extraction with and without posterior chamber intraocular lens implantation].

Three-hundred and fourteen eyes that underwent extracapsular cataract extraction (ECCE) were analyzed. ECCE with posterior chamber intraocular lens implantation (PC IOL) was performed in 168 eyes and ECCE alone was performed in 146 eyes. The complications and visual acuity were compared in the two groups. The PC IOL group showed a low incidence of complications. In addition, vitreous loss occurred in 2.38%, cystoid macular edema occurred in 0.6%, and visual acuity recovered to 0.5 or better in 97.62% of the eyes. The incidence of complications in the ECCE group was slightly higher. The postoperative visual acuity in the ECCE group was lower than that in PC IOL group.

Adult↗
PubMed1993

[A fatal case due to intoxication with seven drugs detected by GC-MS and TDx methods].

A twenty-year-old woman was suspected to ingest large amounts of 4 kinds of over-the-counter analgesic and antipyretic drugs, and was found dead. Drugs and poisons were screened by TOXI-LAB drug detection system in the serum and urine, and analyzed by GC-MS and TDx system in the blood, urine, organ tissues and contents of stomach and small intestine. The concentrations (microgram/g) of bromisovalum, apronalide, ibuprofen, ethenzamide, acetaminophen and caffeine in the heart blood were 36.5, 7.58, 43.1, 16.9, 1.22 and 177, respectively. Salicylic acid concentration in the serum was 82.1 micrograms/ml. The concentrations of bromisovalum and caffeine are high enough to be lethal levels. Neither fatal pathological findings nor traumatic wounds were seen. The overdose of both bromisovalum and caffeine and synergistic, additive or combined effects of other 5 drugs above are considered to be her cause of death.

Acetaminophen↗
PubMed1993

[ABO blood grouping of fingerprint by means of immunohistochemical procedure].

For the purpose of ABO-blood typing on fingerprints, the detection of blood group substances in fingerprints attached on nitrocellulose filter or paper was performed immunohistochemically using avidin-biotin-peroxidase complex (ABC) method. At first, it was fundamentally tested whether ABO-blood typing could be specifically performed for the fingerprints of known ABO blood group, being made experimentally on nitrocellulose filter or paper, and the effect of fixation and paper quality for the detection was also examined. And, ABO-typing was carried out using transferred fingerprints from a slide glass to a nitrocellulose filter. Moreover, using a fingerprint of unknown ABO-type, serially repetitive blood typing (three times) was compared to individually performed grouping (three tests) after dividing a single fingerprint into three parts. In addition, the method of serial ABO-blood typing after the morphological detection of fingerprints by ninhydrine was also considered. As results, according to primary antibodies applied, ABH activities were specifically detected in the fingerprints on nitrocellulose filter and paper. For the fixation procedure of very minute blood group substances to paper, heat fixation was the most effective and methanol fixation was also available. The intensity of immunostaining of fingerprints decreased according to the deterioration of paper quality used. And, the transferred fingerprints on nitrocellulose filter were also specifically typed. Serially repetitive blood typing (anti-B-->anti-A-->anti-H) was possible to fingerprints on nitrocellulose filter, but it gave poor results to those on paper. To overcome this difficulty, after being detected morphologically by ninhydrine, iodine or aluminium powder and decolored thereafter, a fingerprint on paper was divided into three parts and specific blood typing was possible. As for the double blind test to fingerprints on paper, 22 out of 35 fingerprints were specifically typed and the rate of correct typing was 62.9%. From these results, it can be considered that this method is available for ABO-typing of fingerprints of secretor type in forensic practice.

ABO Blood-Group System↗
PubMed1992

[Isolation of verocytotoxin-producing Escherichia coli from cattle and pets].

During a three-year period (1987 & 1990), a comprehensive attempt was made to isolate verocytotoxin-producing Escherichia coli (VTEC) from 96 bovine, 89 porcine, 67 canine and 18 feline cases suffering from gastroenteritis. VTEC were isolated from 11 cows and 1 cat. Bead-ELISA and oligonucteotide probes were used to type the verotoxins (VT) and it was found that the VTEC strains from cows produced not only the currently recognized VT1, VT2 and VT2vh but also produced two new VT's tentatively designated as VTx and VTy. The strain from the cat produced VTy. Serotyping of the strains revealed that some animal strains belonged to similar serotypes as those isolated from human patients with hemorrhagic colitis and hemolytic uremic syndrome. Cattle, especially cows, and domestic pets apparently are reservoirs of VTEC and probable sources of infection in our country as has been previously documented in Canada and USA.

Animals↗
PubMed1992

[Isolation of vero-cytotoxin-producing Escherichia coli from cattle and serotyping and toxin-typing of the isolated strains].

Two hundred and sixty-six piglets with diarrhea (from 4 farms), 73 healthy pregnant pig (from 2 farms), 27 calves with diarrhea (from 9 farms) and 47 healthy milk cows (from 1 farm) were examined for Vero-cytotoxin-producing Escherichia coli (VTEC), and 52, 11, 15 and 67 strains of VTEC were isolated from 17 piglets, 11 pregnant pigs, 6 calves and 23 milk cows, respectively. All VTEC strains from the piglets produced only VT2vp, while the strains from the healthy pigs did not produce VT2vp, but did VT1 and/or VT2. Most VTEC strains from calves and cows produced VT2vhb and some produced VT2 and VT1. Serotyping of the isolated strains showed that many strains from the piglets belonged either O139:H1, O141:H4 or O141:HUT, but the strains from the pigs were either R-form or O-untypable. Many strains from the calves and cows were serotyped into O116 or O113, but there were several R-form and O-untypable. From these results, it is suggested that VTEC strains, especially from the pregnant pigs, calves with diarrhea and healthy milk cows, which produced the same type of Verotoxins to that produced by human isolates, may become sources of human infections.

Animals↗
PubMed1992

Application of hemoglobin analysis by CO-oximeter to medico-legal practice with special reference to diagnosis of asphyxia.

Hemoglobin analysis by CO-oximeter is more easily and rapidly available method than other spectrophotometric or GC methods. In the present study, the possibility of diagnosis of asphyxia by CO-oximeter was examined experimentally using rabbits. When measured value of oxyhemoglobin (O2Hb) was lower than about 20% and that of deoxyhemoglobin (HHb) was, on the other hand, higher than about 80%, being accompanied by low measured values of both methemoglobin (MetHb) and CO-hemoglobin (COHb) (< 5%), asphyxia should be firstly considered as the cause of death. To the corpses found at the scene of fire, three chief factors causing death such as burns, suffocation due to oxygen deficiency and intoxication by poisonous gases may have occurred simultaneously, and through measuring the values of O2Hb, COHb, MetHb and HHb, the main lethal factor could be selected out among these three factors above and the cause of death could be examined much more closely. Because of easy and rapid analysis, it could be considered that CO-oximeter was useful for forensic practices and police activities.

Animals↗
PubMed1992

[A preliminary approach of the malocclusion of second permanent molar].

We examined 2183 cases of orodental malocclusion in our clinic. 420 cases of deep-overjet of second permanent molar were found. In this paper we discussed the sex and age of the patients, distribution of tooth position, ratio, degree of overjet, causes of malocclusion, malocclusion condition, endangerment, prevention and treatment.

Activator Appliances↗
PubMed1992

[Chemical studies on immunologically active polysaccharides of Ganoderma lucidum(Leyss. ex Fr.) Karst].

BN3B, the polysaccharide component of the fruit of Ganoderma lucidum, has been shown to have immune activity. From BN3B four homogeneous polysaccharides were separated and purified. Chemical studies on the main components BN3B1 and BN3B3 indicated that BM3B1 contained only glucose and should be a glucan containing beta-(1----6) and (1----3)glycoside bonds and that BN3B3 was an arabinogalactan containing beta-(1----6) and (1----3)glycoside bonds.

Adjuvants, Immunologic↗
PubMed1992

[An autopsy case of fatal nicotine poisoning].

A fatal case of nicotine poisoning is reported in which a 44-year-old female committed suicide in a short time by taking orally the eluate from tobacco. External examination showed no abnormal findings except for markedly dark red-purple postmortem lividity, and internal examination demonstrated no pathological changes but the signs of sudden death. Through the toxicological investigation by GC and GC-MS, however, nicotine was detected in the solution which she had taken orally and in the blood, urine and the contents of the stomach and small intestine. The nicotine concentrations of the blood, urine and contents of stomach and small intestine were 6.3 micrograms/ml, 1.5 micrograms/ml, 30 micrograms/ml and 71 micrograms/g respectively, and enough to be lethal.

Adult↗
PubMed1992

[The research of orthodontic therapy in second molar overjet]

The malocclusion of overjet of second molars is very frequent,When the 2183 patients were examined,we found that the above-mentioned malocclusion had 420 cases.It accounts for 19.22%.The factors led this malocclusion are many.The cases of buccoversion of second molar in maxilla and normal of this tooth in mandible are 257.That upper second molar is buccoversion and lower second molar is linguoversion.all in one side,are 104 cases.Upper normal and lower linguoversion are 24.Buccoversion of two second molars only in upper dental arch and lingual position of second molar in one side of lower dental arch are 31.Even that bilateral lower second molars are linguoversion and this tooth of upper jaw in one side is buccoversion are 4.Therefore,we must adopt various technique according to malposition condition of second molar.This paper will describe five corrective.

Journal Article↗
PubMed1992

Transcatheter ablation of the accessory pathway in 2 patients with life-threatening tachyarrhythmias.

Successful transcatheter ablation of an accessory pathway in 2 patients with life-threatening tachyarrhythmias is reported. In both cases, electrophysiological evaluation documented the posterior septal location of the anomalous atrioventricular connection. Radiofrequency current energy was delivered just below the coronary sinus orifice, and resulted in disappearance of preexcitation. During the follow-up, both patients remained free from tachyarrhythmias on no medication, and serial electrocardiographic recordings confirmed the persistent absence of preexcitation. It is concluded that ablation of accessory pathways can be accomplished easily and without risk by means of the radiofrequency catheter technique. This therapeutic modality requires a short hospitalization and can be recommended as the first option in patients with the Wolff-Parkinson-White syndrome.

Adult↗
PubMed1991

Hepoxilin A3 induces heat shock protein (HSP72) expression in human neutrophils.

In this paper we show that hepoxilin A3 induces the expression of heat shock protein expression in human neutrophils at a concentration of 100 nM using Western blotting techniques employing the use of a commercial monoclonal antibody to HSP72. No regiospecificity was observed as the 8S enantiomer of HxA3 was as active as the 8R enantiomer of HxA3. Comparison of the effects of HxA3 with 12S-HETE and PGA1 indicated that HxA3 was as effective as 12S-HETE although PGA1 was essentially inactive at the same concentration used for these 12-lipoxygenase products.

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid↗