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Biomedical subjects

Z Lin

Publications and source records attributed to Z Lin.

At least 307 records · Page 17Linked to original sources
PubMed1995

[Measurement of gamma-interferon and its production capability in pleural effusion].

IFN gamma levels and IFN gamma production capability were studied. The results showed that IFN gamma levels, IFN gamma production in the tuberculous pleurisy (n = 30) were significantly higher than those in the malignant pleural effusion (n = 23) and the pleural transudate. However, there was no detectable IFN gamma in the blood, IFN gamma production were lower in the blood than in the pleural effusion. The finding suggests that IFN gamma may play an important role in local anti-mycobacterial infection.

Adolescent↗
PubMed1995

[Studies on the cell models of differentiation reversal phenotypes of human rhabdomyosarcoma].

We have observed the features of malignant phenotypes in human rhabdomyosarcoma RD cells. These cells exhibited high growth rate, aberrant relation between cell proliferation and myogenic terminal differentiation, blockage of myofibrillogenesis, inhibition of gap junctional intercellular communication (GJIC) and inhibition of transcriptional expression of gap junction gene, connexin 43 (Cx43). By utilization of single cell subclone separation and cDNA cell transfection techniques, we obtained several RD subclones, among them 3 were representative of differences in phenotypes and were selected for further study. They were single-cell subclone RDL6, RDL3, and a Cx43 transfectant clone of RDL6, the RDL6/C-4. Through determination of cell growth doubling time, co-stain of 3H-TdR autoradiography with myosin heavy chain (MHC) immunofluorescence, immunofluorescent cytochemical examination and slot-blot analysis, it was demonstrated that significant differences existed between each of the 3 subclones. They were differnet in cell growth rate, cell proliferation and terminal differentiation coupling relation, progress of myogenesis, functional expression of Cx43 gene, and expression of proto-oncogenes, c-myc, Ha-ras and c-met products, therefore they could be used as cell models in the study of reverse transformation of rhabdomyosarcomas. The reversal differentiation markers, their examination approaches and practical value were discussed.

Cell Transformation, Neoplastic↗
PubMed1995

[Clinicopathologic features and diagnosis of mixed type hepatocellular carcinoma].

Hepatoma cases (n = 130) were analyzed by means of histochemical and immunohistochemical stainings. There were 99 cases of hepatocellular carcinoma (HCC), 15 cholangiocarcinoma (CC), and 16 combined HCC and CC (HCC+CC). The clinical features and the cases accompanied with hepatitis and/or liver cirrhosis in the non-tumor liver tissue of HCC+CC cases were intermediate between HCC and CC cases. Histologically, in HCC+CC cases, there were 4 cases with trabecular, 4 with pseudoglandular, 3 with solid type. In these 11 cases, the CC occupied less than 10% of the neoplasm. These cases were designated as HCC+CC type I. There was no obvious stromal fibrosis. The rest 5 cases of HCC+CC cases showed tubular carcinoma in which the CC occupied more than 10% of the tumor. These cases were designated as HCC+CC type II. There was significant fibrosis in the stroma. In all HCC+CC cases only the CC region was positive for mucin and EMA staining. Nearly 70% of the HCC+CC cases had intracytoplasmic glycogen in the HCC area. Transition areas between HCC and CC in both type I and type II HCC+CC cases were observed and they were mucin negative but EMA positive. In conclusion, HCC+CC has both HCC and CC regions with the characteristics of HCC and CC, respectively. Histochemical mucin staining and immunohistochemical EMA staining are helpful in the detection and diagnosis of the HCC+CC.

Adult↗
PubMed1995

[Studies on elastase from Flavobacterium. I. Strain screening and enzyme purification].

132 strains bacteria secreting extracellular elastase were isolated from soil samples, 5 of them possessed considerably high elastiolytic activity of more than 100 u/ml. The highest-yield strain No. 17-87 was characterized as Flavobacterium odoratum, studies on the condition of elastase production revealed that its optimum carbohydrate and nitrogen source were glucose and casein respectively, and that it could utilize fowl ferther meal and wheat bran to give 80% relative yield. The culture exhibited maximum elastase activity at 26 degrees C for 21 hours, the productivity could be increased when the aeration was improved. The PAGE-homogenous elastase preparation was obtained from the culture broth by (NH4)2SO4 fractionation, DEAE-cellulose column chromatography and gel filtration on Sephadex G-75. The molecular weight was determined to be 21380 by SDS-PAGE, the elastiolytic activity was optimal at pH7.4 and 50 degrees C. The enzyme was stable over the range of pH4.5-9.5 and below 40 degrees C, but the activity was inhibited completely by Fe3+, Zn2+, Cu2+, Cr3+, Co2+, Ni2+, Hg2+, Ag+.

Flavobacterium↗
PubMed1995

Functional expression of gap junction gene Cx43 and the myogenic differentiation of rhabdomyosarcoma cells.

Rhabdomyosarcoma (RD) cells express low levels of the gap junction protein connexin 43 (Cx43), and its mRNA, and display very weak gap junctional intercellular communication (GJIC) as detected by Cx43 immunofluorescence, slot-blot and dye-transfer methods. These cells grow rapidly and show aberrant and incomplete myogenic differentiation. To investigate the role of gap junctions in these cells, the expression of Cx43 with relation to cell growth and myogenic differentiation in RD single-cell subclones-RDL3 and RDL6 is studied. The subclone RDL3 grows slowly and displays better myogenic differentiation. The expression of Cx43, its mRNA and the GJIC in RDL3 is comparable to that in normal myoblasts. Another subclone RDL6 which grows rapidly, but is poorly differentiated, expresses very low levels of Cx43 and its mRNA, and very weak GJIC. By using the calcium phosphate precipitate transfection technique, a full-length cDNA-encoding Cx43 and a pSV2neo have been introduced into the RDL6 cells. Several stably transfected clones have been obtained. A stable Cx43-transfectant clone RDL6/C-4 expresses high level of Cx43 and its mRNA, and results in dramatic increase of GJIC. These cells grow slowly but display the enhanced myogenic differentiation. A correlation between the down-regulation of Cx43 gene expression and a reduced expression of myogenic differentiation in RD cells is is demonstrated. Forced expression of Cx43 not only inhibits cell growth but also correlates with the improved myogenic differentiation of RD cells.

Cell Communication↗
PubMed1995

Cat 6 mo increases symptoms: online physician charting and more.

The physicians of the Children's Asthma Center and the computer scientists of Information Services at Texas Children's Hospital set out to design a system that is comfortable to use, structured enough to effectively measure outcomes, yet flexible enough to conserve the individuality of the patient. To achieve these goals, we examined how the differential diagnosis process is applied to clinical decision making and implemented it in a clinical workstation. Unique patterns representing the state of the patient's disease are formed by dynamically selecting pertinent sets of observations, assigning attributes to these observations, and describing relationships between observations and/or sets of observations.

Asthma↗
PubMed1995

[Surgical treatment of primary cardiac tumors].

From Jan. 1962 to Nov. 1994, 130 patients with primary cardiac tumors were performed in Shanghai Chest Hospital. There were 48 male and 82 female patients ranging in age from 16 to 73 (average 43.8 years). 123 (94.6%) had benign and 7 (5.4%) were malignant tumor. Cardiac myoxmas accounted for 91.1% of all benign neoplasias. 5 patients with benign and 1 patient with malignant tumor died early (within 30 days of operation). Of the 4 patients who died late, 3 had had malignant disease.

Adolescent↗
PubMed1995

[Malignant melanoma of nasal cavity: report of 24 cases].

The malignant melanoma of nasal cavity is one of the refractory diseases. The malignant degree and the recurrent rate of this disease are high. And it is very easy to make misdiagnosis. This article reviewed the clinical and pathological data of malignant melanoma of nasal cavity for 20 years from 1971 to 1991. All the patients were followed up. We found that there were altogether 24 cases of this disease which covered 0.02% of all the inpatients during that period of time. The pathological characteristics of this tumor showed that there was a typical fore-melanin body in different tumor cells. If we pay attention to the minor and grained melanin, misdiagnosis can be avoided. Surgery is the first choice for this disease. And the post-operative radiotherapy, laser therapy gives a better result.

Adult↗
PubMed1995

[Posterior chamber intraocular lens implantation in diabetics].

93 eyes having undergone extracapsular cataract extraction with posterior chamber intraocular lens implantation were evaluated prospectively. There were two groups of patients, including 38 diabetics with or without nonproliferative retinopathy and 38 nondiabetics. The comparison between the two groups demonstrated that there was no significant difference in intra-operative or postoperative complications. The post-operative corrected visual acuities of 0.5 or better were achieved in 26 of 47 (55.3%) of diabetic eyes and in 30 of 46 (65.2%) of nondiabetic eyes (P > 0.05). Based on this study, it is considered that nonproliferative diabetic retinopathy is not a contraindication of intraocular lens implantation. However, post-operatively, diabetic patients should be followed more closely and if any post-operative complication does occur, it should be treated promptly.

Aged↗
PubMed1995

Nd: YAG laser lysis of the fibrinous membrane and remnant substance on the anterior surface of intraocular lens.

PURPOSE: To determine the effects of Nd: YAG laser to disrupt the fibrinous membrane and remnant substance on the anterior surface of intraocular lens. METHODS: Nd: YAG laser was applied on 23 cases of fibrinous membrane formation and 8 cases of remnant substance on the anterior surface of intraocular lenses (IOL) which had not responded well to steroid therapy. Eighteen cases were male and 13 female. The mean age was 49.7 years (range, 5 approximately 78 years). The interval between IOL implantation and laser therapy was 0.5 approximately 30 months in the fibrinous membrane cases and 3 approximately 10 days in the remnant substance cases. The energy applied was 0.8 approximately 3.0 mJ/exp. with 2 approximately 112 exposures. Mean follow-up period was 3.6 months. RESULTS: Complications during therapy included only 2 cases of slight iris bleeding. Visual acuity after therapy was improved 1 line in 16 cases, 2 lines in 11 cases, 3 lines in 1 case, 4 lines in 1 case and 5 lines in 2 cases. No post-therapy complication was found. CONCLUSIONS: Nd: YAG laser lysis is an effective alternative to remove the fibrinous membrane and remnant substances on the anterior surface of IOL.

Adolescent↗
PubMed1995

[Clinical study of laryngeal and laryngopharyngeal cancers with ultra-weak chemiluminescence analysis].

Ultra-weak luminescence of serum in 30 normal persons and 40 patients (12 laryngeal cancers, 18 laryngopharyngeal cancers, and 10 benign tumour patients) was studied using SHG-1 type photobiological photometer. The results showed that the intensity in malignant tumour group was higher than that of the benign group, which in turn was higher than that of the normal group with significant statistical differences (P < 0.05). The study implied that ultra-weak luminescence test is of value in diagnosis of laryngeal and laryngopharyngeal cancers.

Adult↗
PubMed1995

[Reason and management of posterior capsular breaks during cataract extraction and lens implantation].

PURPOSE: To study the reason and management of posterior capsular bresks during cataract extraction and lens implantation. METHODS: The authors analyse the reasons of posterior capsular brseaks during cataract extraction and lens implantation on 55 eyes and discuss the management according to the different situations. RESULTS: The posterior capsular disruption occurred most frequently during cortical cleanup on 21 eyes (38.2%) and lens implantation on 21 eyes (38.2%). The diameter of the tears was about 3 mm on 39 eyes (70.9%), about 5 mm on 13 eyes (23.6%) and larger than 6 mm on 3 eyes (5.5%). The tears with central defined borders were on 12 eyes (21.8%), with peripheral defined border in one side on 39 eyes (71%), with pooly defined borders on 3 eyes (5.5%). Final visual acuity of 0.5 or better was achieved on 35 eyes (63.6%). CONCLUSIONS: When the complication occurs during the operation, we should use the suitable management according to the different situations in order to reduce the loss of the complication.

Adolescent↗
PubMed1995

[Trabeculectomy combined with extracapsular cataract extraction and posterior chamber intraocular lens implantation].

PURPOSE: To evaluate the effect of trabeculectomy combined with extacapsular cataract extraction and posterior chamber intraocular lens implantation (triple surgeries) on the patients of coexisting cataract and glaucoma. METHODS: 18 cases (19 eyes) of coexisting cataract and glaucoma treated by the triple surgeries were reviewed. Mean follow-up was 14.7 months (rangs from 2 to 20 months). RESULTS: Intraocular pressure was controlled satisfyingly in all cases except one case required additional treatment of the anti-glaucoma drug. 78.9% of patients achieved 0.3 or better visual acuity. The operation complications consist of anterior uveitis and hyphaema. However, all of them were controlled within 14 days. CONCLUSION: The triple surgeries are safe and have definite effect on controlling the IOP and improving visual acuity.

Adult↗