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Biomedical subjects

Z Li

Publications and source records attributed to Z Li.

At least 991 records · Page 55Linked to original sources
PubMed1998

[Change of cell proliferation after the denervation laryngeal adducent muscles in dogs].

OBJECTIVE: To investigate the changes of cell proliferation state after denervation of laryngeal adducent muscles in dog. METHODS: The muscles were obtained from 8 dogs with recurrent lanyrgeal nerves injured. The cross sectional areas of muscle cells were measured by VIDS Image Analysis System in HE stained sections. A quantitative collagen/muscle ratio was measured by TJTY-300 image analysis system in Van Gieson stained sections. The percentages of S phase cell nucleus were calculated by image cytometry/DNA quantitative analysis with CAS 200 System. RESULTS: It showed that the cross sectional area of muscle cells decreased gradually after denervation (out-standing in 6 months after denervation) and collagen/muscle ratio increased significantly with time. The percentage of S phase nucleus was high at 5 months and then decreased. CONCLUSION: The proliferation of thyroarytenoid muscle and lateral cricoarytenoid muscle cell of dog remained at high level in 5 months after denervation and then slowed down and atrophy of muscles occurred 6 months after denervation. This suggests that nerve repair should be performed early. Examination of cell proliferation might indicate if the denervation of cells irreversible. It is helpful in choosing proper surgery and estimating the effect of nerve repair.

Animals↗
PubMed1998

Machine vision monitoring of plant growth and motion.

The tomato plant was used as a model to study growth and movement due to temperature changes in the environment. A morphological feature, plant top projection canopy area (TPCA), was used to characterize the plant growth and movement. Three temperature regimes (normal temperature, low temperature, and a step change from normal to low temperature) were used for the study. It is found that the plants have significant cyclic canopy movement. In addition, both plant growth, which is represented by canopy expansion, and canopy movement are affected by air temperature. The response of the plant to a step change of air temperature was also documented.

Carbon Dioxide↗
PubMed1998

[Peroxidase in Glycyrrhiza uralensis Fisch. at primary stage of sprouting].

Changes of the peroxidase in Glycyrrhiza uralensis were detected by electrophoresis and spectrophotometric analysis at the primary stage of sprouting. The result shows that during the process of seed sprouting and radicle elongating, the activities of peroxidase keep increasing, some new bands being detected and some original bands enhancing. The changes take place slowly within 24 h, but become faster after 24 h.

Electrophoresis, Polyacrylamide Gel↗
PubMed1998

[A research on chemical dynamic changes and drug efficacy of SMS compound prescription: chemical researches on shenmaisan prescription (I)].

To clarify the material base of the distinct curative effect of Shengmaisan (SMS) compound prescription, a study was made on the dynamics of chemical constituents in the decoction prepared according to the compound prescription. From the decoction a new compound was isolated and identified by UV, IR, MS and NMR as 5-hydroxymethy-2-furaldehyde (5-HMF). The study suggests that the distinct curative effect of SMS compound prescription hinges on a material base which is different chemically from that in the other three single drugs used separately, thus presenting scientific criteria for rational compatibility and quality check in making preparations on compound prescriptions.

Drug Combinations↗
PubMed1998

[The effects of allitridum on the proliferation of rabbit aortic smooth muscle cells in vitro].

The effects of allitridum on the proliferation of cultured rabbit aortic smooth muscle cells (SMC) were studied with SMC incorporation data of 3H-thymidine(3H-TdR) and contents of superoxide dismutase(SOD), lipid peroxide(LPO), prostacyclin(PGI2) and cyclic adenosine monophosphate(cAMP) in medium. The results showed that allitridum could inhibit SMC proliferation by increasing SOD activity, decreasing LPO, raising PGI2 and cAMP(all P < 0.05-0.01), and the inhibitory action was accompanied by the dose-effect relation.

Allyl Compounds↗
PubMed1998

[Preliminary analysis and isolation of genomic total DNA in Fritillaria thunbergii Miq].

Genomic total DNA of leaf, fresh scale and dry scale in Fritillaria thumbergii were isolated by two different methods and analysed by agarose gel electrophoresis. The results show that the DNA contents are different under these two isolating methods. Two bands besides the band of genomic total DNA have been found by electrophoresis, these two bands deserve further study.

DNA, Plant↗
PubMed1998

[Metabolism in Arctium lappa L. at the primary stage of germination].

OBJECTIVE: To investigate the metabolic changes of the seeds at the primary stage of germination in Arctium lappa. METHOD: By means of electrophoresis the changes of peroxidases and soluble proteins were assayed at the primary stage of germination. RESULTS: The color of some original bands was enhanced, some original bands disappeared, and some new bands were detected in peroxidase patterns during the germinating process. The color changes were the main expression in soluble protein patterns. The bands of soluble proteins changed most obviously at 96 h and 120 h after germination. CONCLUSION: It follows that the metabolic process in the seeds of Arctium lappa at the stage of germination is complex and 96 h and 120 h after germination are the key times of metabolism.

Asteraceae↗
PubMed1998

[A research on chemical dynamic changes and drug efficacy of shengmaisan compound prescription: chemical researches on shengmaisan prescription(II)].

The content changes of 5-hydroxymethyl-2-furaldehyde(5-HMF), a new compound reported in the previous paper, in eight prescriptions of different composition were further determined by HPLC with a view to find the chemical dynamic changes and compatibility of medicines. The contents of 5-HMF in different combinations of Radix Ophiopogonis and Fructus Schisandrae, different boiling times and boiling frequency were examined. The results indicated that 5-HMF was produced in the boiling process of Radix Ophiopogonis and Fructus Schisandra combined. The contents of 5-HMF would increase with the increase of Radix Ophiopogonis amount, reaching the highest value after 1.5 h boiling and two times of boiling.

Drug Combinations↗
PubMed1998

[Identification of commercial Chinese medicine ziwan].

OBJECTIVE: Commercial samples of Ziwan have been investigated and identified to find out the original species in circulation. METHOD: Comparative histological and morphological identification. RESULT: The main original plant species are Aster tataricus, Ligularia fischeri, L. hodgsonii, L. intermedia, L. wilsoniana, L. sibirica var. araneosa, L. veitchiana and L. caloxantha. CONCLUSION: The root and rhizome of A. tataricus are used as Ziwan in most areas of China; but those of Ligularia species are used as Ziwan(named Shanziwan) in southwest, and part of northwest and northeast areas of China.

Asteraceae↗
PubMed1998

[Recurrent pheochromocytoma].

OBJECTIVE: To improve the diagnosis of recurrent pheochromocytoma. METHODS: To study 11 cases of recurrent pheochromocytoma treated from 1970 to 1996. RESULTS: The recurrence was due to the metastasis of malignant tumor and the multifocal occurrence of benign one. Extraadrenal pheochromocytomas tended to occur at several sites or metastasize. The metastatic places usually no show pheochromatic tissues, and the recurrent benign tumors were liable to grow at extraadrenal regions. The nature of recurrent tumors should be judged according to variant factors. CONCLUSION: The recurrent pheochromocytomas are not malignant in nature, but they have considerable malignant tendency and should undergo intensive survelliance and regular examinations especially for the primary or recurrent extraadrenal pheochromocytomas.

Adolescent↗
PubMed1998

[Protease inhibitory activities of nasal secretion on house dust mite allergen].

OBJECTIVE: To elucidate the interaction of protease inhibitors and house dust mite allergen in vitro. METHODS: Nasal secretion was collected by suction from patients with house dust mite nasal allergy, diluted immediately with PBS pH 7.0, mixed with vortex for 10 minutes, and then centrifuged at 2,000 g for 10 minutes. The supernatant was kept at -80 degrees C for use. Synthetic low molecular weight fluorogenic peptide substrates were applied to evaluate protease activities. Crude mite extract and other proteases were used, each of them was preincubated in the presence or absence of nasal fluid or other protease inhibitors at 37 degrees C for 30 minutes. The intensity of fluorescence of aminomethylcoumarin released from the cleaved substrate was measured. RESULTS: Nasal fluid exhibited mild trypsin-like and other protease activities. It inhibited the activities of trypsin and trypsin-like serine proteases and papain-like cysteine proteases in crude mite extract in a dose-dependent manner. CONCLUSIONS: Nasal fluid possibly plays a role in inhibiting penetration of allergen into the nasal mucosa by producing protease inhibitor-protease complex in the nasal mucus layer of patients with mite allergy.

Adolescent↗
PubMed1998

[Treatment of the unilateral paralysis of vocal fold--comparison between laryngeal framework surgery and reinnervation].

OBJECTIVE: To compare the effects of laryngeal framework surgery and reinnervation in the treatment of unilateral paralysis vocal fold. METHODS: Twenty-three cases of unilateral vocal fold paralysis were operated with laryngeal framework surgery and reinnervation and the voice assessment, acoustic analysis, laryngoscopy, electromyography were done pre- and postoperatively. RESULTS: The short and long term curative effects of the laryngeal framework were good, but with regard to long term result, the reinnervation that can recover the thickness, elasticity and tension of vocal fold was better, and the earlier was the operation done, the better would be the result. CONCLUSION: These two operations were both effective in the treatment of unilateral paralysis of vocal fold, but it should be selected according to the requirement of patient, the pattern of damage, the degree of paralysis and movement of cricoarythenoid joint.

Adult↗
PubMed1998

[Prevention and management of chylous fistula after neck dissection].

OBJECTIVE: To evaluate the effect of treatment for chylous fistulas after neck dissection, as a means of reasonable option of therapeutic measures. METHODS: Of 37 chylous fistulas, 30 were left-sided and 7 were right-sided. Conservative therapy which included closed-wound drainage and local pressure dressing and/or low-fat nutritional support was employed in 26 cases, and 50% glucose was injected into the neck basic wound bed in 2 cases, to promoto the chylous fistula healing up. Operative ligation was performed in 7 cases. Open-wound and packing in 4 cases. RESULTS: Eighty-six percent chylous fistula occured from 1 to 3 postoperative days. Of 26 chylous fistulas with conservative management, 15 cases were successfully treated, the mean duration for healing was 9.7 (4-30) days, the remaining 11 cases failed to had and were further treated surgically. Seven cases with operative ligation were successful, and the other 4 cases with open-wound and packing had delayed healing, the mean duration was 8.5 (7-10) days. CONCLUSION: The majority of patients with chylous fistulas could be treated reasonablly without increasing the hospital days. The conservative therapy should be employed in cases with a maximal production of chyle below 500 ml a day. Operative ligation should be done early in cases with a maximal production exceeding 500 ml a day and with conservative treatment failure for a few days. The open-wound and packing should be used in chylous fistulas persisting for more then 7 days.

Adolescent↗
PubMed1998

[Application of CT virtual endoscopy in larynx].

OBJECTIVE: To evaluate the clinical application of CT virtual endoscopy in larynx. METHODS: The virtual endoscopy images were compared with that of actual laryngoscopy in 16 patients(4 normal larynx, 9 laryngeal carcinomas, 1 bilateral glottic polyps, one laryngeal fibrous hyperplasis and 1 airway striction caused by infiltration of the laryngeal carcinoma after laryngectomy). All the lesions were pathologically proved. RESULTS: CT virtual endoscopy showed the normal anatomical structure of larynx as actual laryngoscopy. The location and extension of the lesions of larynx showed in CT virtual endoscopy agreed with that of actual laryngoscopy. In addition, CT virtual laryngoscopy revealed the structure of subglottic region and distal portion of strictured airway from feet to head, which were not available in actual laryngoscopy. CONCLUSION: CT virtual laryngoscopy is a good complemental method of fiber optic laryngoscopy.

Glottis↗
PubMed1998

Diagnosis and treatment of pulmonary hamartoma.

From 1970 to 1997, 67 patients with pulmonary hamartoma were operated on in our hospital. There were 38 men and 29 women with a M/F ratio of 1.3:1. The mean age was 47 years (range 21 to 82 years). The peak incidence was 40 to 60 years. 39% patients had pulmonary symptoms: hemoptysis, cough, phlegm and chest pain. All were solitary. 68.7% tumors were located in right lung and 64.2% in upper or middle lobe. 80.6% hamartomas were less than 3 cm in transverse diameter. On chest films, the opacity was homogeneous in 79.1% cases, heterogeneous in 20.9% and calcifications in 9.0%. The vast majority of shadows were heterogeneous on CT films. Operation is the best treatment for hamartomas. Postoperative mortality was 1.5%. There was none recurrences and canceration during the follow-up (mean 14 years).

Adult↗
PubMed1998

[The pathogenesis of infection complicated by acute necrotizing pancreatitis: an experimental study].

OBJECTIVE: To observe the injury of gut barrier function and intestinal bacterial translocation following acute necrotizing pancreatitis (ANP), and to investigate the pathogenesis of infection complicated by ANP. METHOD: Fifteen dogs were colonized with a strain of E. coli JM109 bearing ampicillin-resistant plasmid PUC18, then divided into two groups: control group (n = 7) and ANP group (n = 8). Acute necrotizing pancreatitis was induced by injection of sodium taurocholate and trypsin into the pancreatic duct. RESULT: In the ANP group, as compared with the control group, serum amylase level increased significantly, the lactulose/mannitol ratio was 2-12 times higher, blood and ileum diamine oxidase activity decreased significantly. E. coli population in intestinal mucosal flora increased, while bifidobacterium and lactobacillus reduced significantly. Bifidobacterium/E. coli ratio was reversed. Morphological study revealed areas of hemorrhage and necrosis in the pancreas and shedding of ileal villi. In the control group, blood cultures were negative, and no organism was found in organs except for mesenteric lymph nodes in two dogs. In the ANP group, blood and organ cultures were positive in all dogs and JM109 could be found uniformly too. CONCLUSION: ANP impaired the gut barrier function, which led to bacterial translocation from the gut to the pancreas and other organs, serving as a primary source of infection secondary to ANP.

Animals↗
PubMed1998

[The distribution of nerve endings in the human cervical nerve roots].

OBJECTIVE: To find out the distribution of nerve endings in the dural sheath of cervical nerve roots and to study the mechanism of cervical radicular pain. METHOD: 2069 20 microm serial sections were made from 39 cervical root-dorsal root ganglion specimens obtained from 18 postmortems. By using optical microscope and Suzuki silver impregnation method and Klüver-Brrera method, we examined the morphology, quantity and distribution of the nerve ending in the nerve sheath of the root, dorsal root ganglion (DRG) and the spinal nerve of these sections. RESULT: There were several kinds of nerve endings such as free nerve endings in the nerve sheath of the root, the dorsal root ganglion, and the spinal nerve. Free nerve endings were distributed in the proximal and distal portions of DRG sheath more extensive than that in other parts of the dural root-sheaths. CONCLUSION: The free nerve endings in the dural root-sheaths especially those in the DRG sheath are thought to play an important role in cervical radicular pain.

Adolescent↗