Dr. Li Zhonghe's experience in acupuncture treatment.
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Acupuncture Points↗
Biomedical subjects
Z Li
Publications and source records attributed to Z Li.
[Expression of telomerase in acute leukemia].
To understand the expression patterns of telomerase activity in different types of acute leukemia(AL) and during remission state, the mono-nuclear cells of different bone marrow samples were isolated by desity gradient centrifugation. Telomerase activity of the samples was assayed by telomeric repeat amplification protocol(TRAP) with the cell extracts and the TRAP products were resolved in PAGE. The results showed that the telomerase activity was at higher levels in the acute leukemia compared with the normal controls. Of the acute nonlymphocytic leukemia(ANLL), the telomerase activity presented as M2a > M5b > M1 > M3b. Of the acute lymphocytic leukemia(ALL), the activity presented as L1 > L2. The activity assayed in M2a and L2 remission was as high as that in the normal group. It indicated that the expression of telomerase was enhanced differently among the subtypes of ANLL and ALL, and was down-regulated during the AL remission. It implicates that the expression patterns of telomerase activity in AL is associated with the different proliferation states and differentiation properties of the AL cells.
Adolescent↗
[The study on relationship between fetal blood flow velocity waveforms and cord blood gas analyses in normal full term pregnancy].
OBJECTIVE: To study the relationship between fetal blood flow velocity waveforms and cord blood gas values in normal full term pregnancy. METHODS: Fetal umbilical artery (UA) and middle cerebral artery (MCA) and abdominal aorta (AbAo) flow velocity waveforms were measured in 45 full term fetuses 24 hrs before selective cesarean section. Resistance index (RI) and pulse index (PI) were calculated, cord blood samples were obtained from the umbilical artery at cesarean section and blood gas values were measured immediately. RESULTS: (1) UA RI had a significant negative correlation with pH and PO2, and a positive correlation with PCO2. (2) MCA RI had a significant positive correlation with pH and PO2, and a negative correlation with PCO2. CONCLUSION: A Doppler study of fetal blood flow velocity waveforms would be a useful method in the evaluation of the fetal status in the uterus.
Aorta, Abdominal↗
[Study on certified reference material of benzene, toluene, o-xylene in charcoal].
This certified reference material (CRM) of benzene, toluene and o-xylene in charcoal is used for quility control and calibration of analytical instruments in the determination of benzene, toluene and o-xylene in air. Homogeneity of benzene, toluene and o-xylene concentrations in the CRM was testes by GC. The stability of the CRM was assessed for one year. The certified values are based on analysis made by a group of independent laboratoies using GC method.
Benzene↗
[Study on the relationship between deoxyribonucleoside triphosphate (dNTP) pools and cell transformation].
Deoxyribonucleoside triphosphate (dNTP) pools were measured in normal BALB/c3T3 cells, transformation-treated cells and transformed cells with reverse-phase HPLC. The fluctuation of dNTP pools was similar after cells were treated with alkylating mutagens glycidyl methacrylate (GMA) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The gap between (dGTP + dATP) pools and (dTTP + dCTP) pools was greatly intensified. The measurements also indicated that the dNTP pools in transformed cells were quite different from those in normal cells. The results suggest that dNTP pools may play an important role in cell transformation.
3T3 Cells↗
[Study on the changes of heart-rate in lead-exposed workers].
ECG examination and questionnaires survey were performed among lead-exposed workers in order to assess whether moderate occupational lead exposure may cause autonomic nervous system (ANS) dysfunction. The tests included; 1. heart-rate response to Valsalva manoeuvre (HR-V); 2. heart-rate variation during deep breathing (HR-DB); 3. immediate heart-rate response to immediate stand up (30:15 or Max:Min). The results did not show any evidence that lead exposure is related to ANS dysfunction. There was no significant difference between the exposed workers and controls who were with the same ages (P > 0.05). The important cause for ANS dysfunction was aging.
Adult↗
[Study on the transformation of mammal cells induced by glycidyl methacrylate in vitro].
Glycidyl methacrylate(GMA) can induce the phenotype transformation of human embryonic lung fibroblasts. The transformed cells prolifeated rapidly with a higher in the ratio of nucleus to cytoplasm, and their life span extended notably. The transformed colonies exhibited in extensive random orientation and the cells crossing-over. The transformed cells could be agglutinated by lower concentration of ConA and could grow in semisolid agar. The chromosome abberations could occur in transformed fibroblasts. These results suggest that GMA is able to induce malignant transformation of mammal cells, and it may be carcinogenic to human.
Air Pollutants, Occupational↗
[Effects of propyl gallate on histopathology of liver from mice exposed to trinitrotoluene].
In addition to the hepatocellular edema and cytoplasmic eosinophilia, sludging of blood was present in liver of mice exposed to trinitrotoluene(TNT). Single necrosis of the partical liver cell was seen occasionally. Liver damage induced by TNT was significantly alleviated by orally administrated propyl gallate(PG). Futhermore, PG can promote the regeneration of the hepatocytes following TNT-exposed mice. The results suggest that PG showed a protective effect on the histopathologic changes of liver injury induced by TNT.
Animals↗
[Simultaneous extraction of tea-polyphenols and caffeine from green tea].
Tea-polyphenols and caffeine were extracted simultaneously from green tea. The factors influencing on the process of impregnation and extraction were studied. The result indicated that the content of tea-polyphenols and caffeine in tea was increased with the duration of extraction and decreased with the frequency of extraction. The authors discuss the effect of pH on the precipition of calcium-tea-polyphenols.
Caffeine↗
[Effect of Ox-LDL on cell cycling phases and PCNA, P53, P27 and c-erbB-2 expression in cultured human arterial smooth muscle cells].
It was found in our previous study that oxidative modification LDL (Ox-LDL) could stimulate the proliferation of cultured human arterial smooth muscle cells (SMC). Yet, the mechanism responsible for the SMC proliferation induced by Ox-LDL is not clear. Proliferating cell nuclear antiger (PCNA), P53 and P27 are the key regulatory factors of cell replication. In order to observe the effects of Ox-LDL on cell cycling phase and PCNA, P53, P27 and c-erb B-2 expression in SMC, we used the flow cytometric method in the present study on the proliferation of cultured human SMC induced by Ox-LDL. The results showed a relation between the Ox-LDL mediated SMC proliferation and the cycling phase shifting. The relative number of S phase cells in the Ox-LDL group was higher than that of the control group (22.9% vs 15.7%). Ox-LDL mediated SMC proliferation was accompanied with the increasing expression of PCNA. The percentage of specific PCNA positive FITC cells in the Ox-LDL group was significantly higher than that of the control group (12.6% vs 6.5%). PMA, an activitor of protein kinase C (PKC), stimulated SMC proliferation and increased the PCNA exression in cultured SMC, while the PKC inhibitor, F109203X, significantly decreased the PCNA expression in SMC (PCNA positive cells 13.4% vs 0.4%). No changes were observed in the expression of P53, P27 and c-erb B-2 in the cultured proliferating SMC induced by Ox-LDL. In all, the results suggest that the OX-LDL mediated SMC proliferation is related to increasing S Phase Cells and involved in the PCNA expression which might undergo the PKC cellular signal transduction pathway.
Aorta↗
[Clinical study on pelvic venous congestion syndrome after tubal sterilization].
OBJECTIVE: To study the clinical features, diagnosis and therapy of pelvic venous congestion syndrome after tubal sterilization. METHODS: By using laparoscopy combined with transmyometrial pelvic venophogram and samples after operation, pathomorphology of pelvic congestion venous is observed. RESULTS: The coincidence rate of laparoscopic diagnosis is 94.6% (53/56), compared with transmyometrial pelvic venophogram. There is no significant difference for light or severe type patients (P > 0.05). The cure rate of the cases in this group is 60.7%, the improvement rate is 16.1%, total effectiveness rate is 76.8%. The effectiveness rate of operational treatment is 90.1%, conservative treatment is 18.2%. CONCLUSIONS: Laparoscopy is very valuable for the diagnosis of this disease. Laparoscopy combined with transmyometrial pelvic venophogram can be regarded as the major method to diagnosis pelvic venous congestion syndrome. It is recommended that operation for patients with medium and severe symptoms.
Adult↗
[Levels of endothelin and beta-human chorionic gonadotropin and their relationship in patients with pregnancy induced hypertention].
OBJECTIVE: To measure levels of endothelin (ET) and beta-human chorionic gonodotropin (beta-hCG) and analyse the correlation between them in patients with pregnancy induced hypertension (PIH), as well as to investigate their role in the pathogenesis of PIH. METHODS: 32 patients with PIH were matched with 17 normal term singleton pregnant women. ET and beta-hCG levels of above patients and 14 nonpregnant health reproductive age women were determined by radioimmunoassay. RESULTS: ET and beta-hCG levels were significantly higher in patients with PIH (30.35 +/- 14.52 ng/L and 7.35 +/- 4.86 nmol/L) than that of two control groups (P < 0.05), especially in middle and severe PIH (P < 0.05), but no significant different in mild PIH (P > 0.05), compared with normal pregnant group (20.79 +/- 7.08 ng/L and 4.13 +/- 1.91 nmol/L) and nonpregnant group (19.78 +/- 6.66 ng/L and 0 nmol/L). There was no significant difference of plasma ET levels between normal pregnant and nonpregnant women (P > 0.05). beta-hCG in nonpregnant woman hadn't been determined. We did not find any association between the levels of ET and beta-hCG in normal pregnancy (r = +0.2216, P > 0.05), but significant positive correlation in the patients with PIH (r = +0.5418, P < 0.01). CONCLUSION: The increased levels of ET and beta-hCG, as well as their positive correlation, suggested that the dysfunction of the placental cells may be associated with the endothelial cell damage in PIH patients.
Adult↗
An observation on the mimic ecology of the oncomelania hupensis in three-Gorge-reservoir areas.
According to the modulation of three-gorge-reservoir water level, the two observation spots were established at Gaoyang Town and Jianshe Village in Xingshan County of Hubei Province in order to observe the survival and reproduction of the oncomelania under different ecological conditions. Our one-year observation revealed that most settled oncomelania died between June and August in the year and only a few of them survived the winter. The oncomelania hupensis's survival rate in group I, II, III settled in Gaoyang Town was 10%, 1%, 0%, respectively, and the oncomelania Sichuan's 12.5%, 22.5%, 13.5%. The rates obtained from spots in Jianshe Village is 0%, 2%, 0% and 9.5%, 19%, 3%, respectively. The survival rate of oncomelania Sichuan was higher than that of the oncomelania hupensis in the two spots. After draining the feeding pools, the soil samples were harvested and used for egg-hatching. The numbers of eggs that hatched in group I, II in Gaoyang were 1 and 2, respectively. The young oncomelania were found in each spot pool after the drainage, suggesting that the local condition is favorable to the oncomelania and the mating season of each group oncomelania is from April to August with the most active mating time being from May to June.
Animals↗
[High frequency mutation of p53 gene in human gastric cancer and precancerous lesions].
OBJECTIVE: To determine the frequency and type of p53 gene mutation with the progression of human gastric carcinogenesis. METHODS: Single strand conformation polymorphism (SSCP) and DNA sequencing were performed on 56 biopsied tissues, including gastric cancer (n = 23), dysplasia (Dys, n = 16) and intestinal metaplasia (IM, n = 17). RESULTS: p53 gene mutation was detected in 60.1% of cancer, 31.3% of Dys and 11.8% of IM. Mutations covered exon 5-8 without hot spot. GC-->AT transitions (30.4%) and GC-->TA transversions (26.1%) were the major patterns of p53 mutation. Missense (73.9%) and frame shift mutations covered 86.9% of all mutations. CONCLUSION: In the process of human gastric carcinogenesis, multiple factors are involved in p53 gene mutations. The mutation rate increases with the progression of malignant changes in the gastric mucosa. Missense and frame shift mutations are possibly the major cause of p53 inactivation.
Adenocarcinoma↗
[Plasma neuropeptide Y (NPY) levels in patients with gastric and colorectal carcinomas].
OBJECTIVE: To investigate the plasma NPY concentration in patients with gastric and colorectal carcinomas in relation to tumor progression. METHODS: Blood samples were obtained from patients with gastric cancer (n = 18) and colorectal cancer (n = 20). Plasma NPY concentration was determined by radioimmunoassay. RESULTS: The average plasma NPY level in the 38 patients studied (125.3 +/- 31.5 pg/ml) was significantly(P < 0.001) lower than that in 28 healthy control individuals (145.1 +/- 44.1 pg/ml). Decreased NPY levels were correlated with loss (> 3 kg) of body weight (P < 0.01) and tumor size (> 5 cm) (P < 0.05). The stage of gastric cancer, but not that of colorectal cancer, was negatively correlated with plasma NPY level. Plasma NPY level did not correlate with sex, age, blood pressure, depth of tumor invasion, degree of differentiation, lymph node metastasis, hemoglobin and albumin concentrations. CONCLUSION: Plasma NPY level is decreased in patients with gastric and colorectal carcinomas, but it reflects only in part the progression of gastric cancer.
Adenocarcinoma↗
[Clinical analysis of postoperative radiotherapy for stage I endometrial cancer].
OBJECTIVE: To evaluate the role and complications of adjuvant radiotherapy for stage I endometrial cancer after surgery. METHODS: From May, 1986 to December, 1995, 20 patients with stage I endometrial cancer received total abdominal hysterectomy with bilateral salpingo-oophorectomy(TAH/BSO), and additional pelvic lymph node dissection in 5 cases. Most of the patients received radio therapy in 2-4 weeks after surgery. The radiation fields included pelvic lymph nodes with a total dose of 40-50 Gy. RESULTS: All the patients had survived for 10 years. Only one patient developed distant metastases. Acute radiation reaction was observed in all patients. Late radiation-induced damage occured in six patients and was severe in two. CONCLUSION: Postoperative radiotherapy in the management of stage I endometrial cancer is beneficial for the controal of local recurrence but has relatively high frequency of late radiation injury.
Adenocarcinoma↗
[Infection of GBV-C virus among hepatitis and HCC patients].
To study the infection status of GBV-C virus among HBV, HCV, nonA-E and HCC patients, serum of clinical hepatitis patients and HCC patients before operation, and the composition in/beside HCC were collected to examine HGV RNA of the samples, using HGV RT-PCR method. Results showed that among the clinical patients with HBV, HCV, nonA-E and HCC, HGV infection rates were 9% (12/130), 10% (3/30), 17% (4/24), 0% (0/24) respectively, suggesting that HGV is a hepatitis virus with pervasive existence and has high superimposition infection rate with HBV and HCV but with little connection with HCC.
China↗
[Molecular analysis of HLA-DR beta polymorphism in Han nationality patients with rheumatoid arthritis].
OBJECTIVE: To analyze the molecular basis for HLA-DR associations with rheumatoid arthritis(RA) in the han nationality of Chinese population. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques were used to dertermine DRB1 alleles in 35 unrelated patients with RA and 100 healthy controls from the Han nationality. RESULTS: The frequency of DR4(DRB1 * 04) was 51.4% in RA patients and 24.0% in the healthy controls(P < 0.01, RR = 3.3). There was a significant increase in the presence of the amino acid sequences QKRAA or QRRAA both in the RA patients overall compared with the healthy group(65.7% vs 30.0%, P < 0.001) and in DR4+ RA patients compared with DR4+ healthy individuals(100% vs 75.0%, P < 0.05). Substitution of residues in QKRAA, V85 and G86 appeared to correlate with relative risk for RA, among the subjects having 0-1 amino acid substitution, RA occurred in 48.8%, whereas in subjects with 2-3 amino acid changes, RA was present in only 16.7%. CONCLUSION: These results suggest that DR4 is strongly associated with RA in the Han nationality, and the dominant effect that determines susceptibility to RA is associated with QKRAA or QRRAA as well as V85 and G86.
Adult↗