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Biomedical subjects

Z Li

Publications and source records attributed to Z Li.

At least 325 records · Page 18Linked to original sources
PubMed2001

[The tissue engineering research on synovialization of non-synovial membrane tendon].

The synovial membrane tissue within the flexor digital tendon sheath of the rabbit's toes was cultured in vitro. The synovial cells suspension of the subculture was cultured with the segments of the non-synovial membrane tendon of the rabbit's toes. Under the light microscopy, scanning electronic microscopy and immunohistochemical examination, the result showed that the synovial cells crawled and covered the surface of the segments of the non-synovial membrane tendons. It is suggested that the non-synovial membrane tendon could transform into a synovial membrane tendon.

Animals↗
PubMed2001

[Research on fibrotic effect of Ni-Ti and 317L alloys in esophagus].

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L alloys in esophagus. The extract fluid from Ni-Ti, 317L alloys was made according to the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily, then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagus embedding test of Ni-Ti, 317L alloys was made according to ASTM standards of U.S.A. The tissue around the alloys was taken at weeks 2 and 12, and the pathologic changes were analysed. The results showed that Ni-Ti, 317L extract could depress the proliferating function of Fb gently, and the depressing action increased gradually with the culturing time. The result of embedding test was in accord with the ASTM standards of U.S.A. completely; the fibrotic membrane around the NiTi, 317L alloys became thinner with embedding time. These findings suggested that the scattering composition of Ni-Ti, 317L in body fluid might not activate the proliferating and secreting function of Fb, and the two alloys could not lead to fibrosis of esophagus aroun them.

Alloys↗
PubMed2001

[Establishment and application of a data management program for BY 960 ambulatory blood pressure monitoring system].

Report of ambulatory blood pressure monitoring may contain a large number of data which is very important for the clinical studies on subjects' blood pressure. Most of the ambulatory blood pressure monitoring systems in use at present are lacking in database managing function, which is very inconvenient to the research work. In accordance with the characteristics of BY 960 ambulatory blood pressure monitoring system (produced by Shenzhen NewEra Tech Co., Ltd.), a set of data management program was established. It can automatically transfer the ambulatory data to database sets and is confirmed to be a practical tool for the research work.

Blood Pressure Monitoring, Ambulatory↗
PubMed2001

[Changes in erythrocyte rheologic properties after mild burn injury].

The changes in erythrocyte deformability and osmotic fragility were studied with an animal model of mild burn injury. The results showed that after the burn injury there was a gradual decrease with time at first and then a recovery in erythrocyte deformability. Maximum deformation index [(DI)max], integration deformation index (IDI) and osmotic fragility of RBCs drawn just after burn were almost identical to those before burn injury. (DI)max and IDI significantly decreased at 1, 2 h but not at 4, 7 h when compared with those before burn injury, however, the RBCs were less deformable at 4, 7 h. Erythrocyte osmotic fragility significantly increased only at 4 h after burn injury when compared at the time points of 0, 1, 2, 4, 7 h after burn with that before burn injury. The results indicated that the lesion in (DI)max and IDI, and the increase in osmotic fragility might be only related to the body fluid factors in this animal model. The decrease in erythrocyte deformability and the increase in erythrocyte osmotic fragility did not synchronize, suggesting that the changes might come about not by the same mechanism.

Animals↗
PubMed2001

Novel short-acting A2A adenosine receptor agonists for coronary vasodilation: inverse relationship between affinity and duration of action of A2A agonists.

Several potent and selective A2A adenosine receptor agonists are currently available. These compounds have a high affinity for the A2A receptor and a long duration of action. However, in situations where a short duration of action is desired, currently available A2A receptor agonists are less than ideal. From a series of recently synthesized A2A receptor agonists, two agonists (CVT-3146 and CVT-3033) with low affinity were selected for further characterization as selective and short-acting coronary vasodilators. Both compounds were selective for the A2A adenosine receptor (AdoR) versus the A1, A2B, and A3AdoR in binding and functional studies. CVT-3146 and CVT-3033 appeared to be weak partial agonists to cause cAMP accumulation in PC12 cells, but were full and potent agonists to cause coronary vasodilation, a response that has a very large A2A receptor reserve. However, the durations of action of CVT-3146 and CVT-3033 were remarkably shorter than those of the high-affinity agonists CGS21680 or WRC0470, presumably due to the relative lower affinity of CVT-3146 and CVT-3033 for the A2A receptor. Indeed, an inverse relationship was found between the affinity of the various agonists for the A2A receptor and the duration of their actions. These data indicate that low-affinity agonists can produce a response that is of equivalent magnitude but more rapid in termination than that caused by a high-affinity agonist. Hence, the low-affinity A2A agonists CVT-3146 and CVT-3033 may prove to be superior to currently available high-affinity agonists as coronary vasodilators during myocardial imaging with radionuclide agents.

Adenosine↗
PubMed2001

[Phenotyping and genotyping studies in a family with the compound heterozygosity for a deletional delta beta-thalassemia and a beta-thalassemia].

OBJECTIVE: To investigate the relationship between genotype and phenotype of a deletional delta beta-thalassemia and explore an approach to rapid prenatal diagnosis for compound heterozygotes of this defect and a beta-thalassemia. METHODS: A total of ten members in a Chinese family who had a 5-year-old propositus with thalassemia major and requested prenatal diagnosis for the second pregnancy were investigated. For genotyping analysis, the deletional delta beta-thalassemia was characterized by gap-PCR method and beta-thalassemia mutations were defined by reverse dot blot(RDB). For phenotyping analysis, hematological data including the RBC indices, quantification of HbF and HbA2 by Hb electrophoresis and the ratio of globin chain Ggamma/(Ggamma+Agamma) analyzed by capillary electrophoresis (CE) were obtained. RESULTS: The propositus inherited her mother's delta beta-thalassemia gene mutation and her father's CD41-42(-CTTT) frameshift mutation. Of nine members in this family screened for this type of deletion, four were positive and the phenotype could be explained satisfactorily by genotype. The results of prenatal diagnosis showed that the fetus was normal and had no beta-globin gene defects in both chromosomes. CONCLUSION: This is the first time to have performed prenatal diagnosis in Chinese family at-risk of compound heterozygotes for beta-thalassemia and delta beta-thalassemia in mailand China. The strategy to analyze the disease presented here may be a valuable reference to the similar problem.

Adolescent↗
PubMed2001

Effect of batroxobin on neuronal apoptosis during focal cerebral ischemia and reperfusion in rats.

We have found that Batroxobin plays a protective role in ischemic brain injury, which attracted us to investigate the effect of Batroxobin on apoptosis of neurons during cerebral ischemia and reperfusion. The apoptotic cells in ischemic rat brains at different reperfusion intervals were tested with method of TdT-mediated dUTP-DIG nick end labeling (TUNEL) and the effect of Batroxobin on the apoptosis of neurons was studied in left middle cerebral artery (LMCA) occlusion and reperfusion in rat models (n = 18). The results showed that few scattered apoptosis cells were observed in right cerebral hemispheres after LMCA occlusion and reperfusion, and that a lot of apoptosis cells were found in left ischemic cortex and caudoputamen at 12 h reperfusion, and they reached peak at 24 h-48 h reperfusion. However, in the rats pretreated with Batroxobin, the number of apoptosis cells in left cerebral cortex and caudoputamen reduced significantly and the neuronal damage was much milder at 24 h reperfusion than that of saline-treated rats. The results indicate that administration of Batroxobin may reduce the apoptosis of neurons induced by cerebral ischemia and reperfusion and afford significant cerebroprotection in the model of focal cerebral ischemia and reperfusion.

Animals↗
PubMed2001

[Chromosomal aberration analyzed by comparative genomic hybridization in nasopharyngeal carcinoma].

OBJECTIVE: To identify genetic alteration in primary sporadic nasopharyngeal carcinoma(NPC) in Hunan. METHODS: Comparative genomic hybridization was applied to investigate the genomic imbalance in a series of 20 cases of primary nasopharyngeal carcinomas. RESULTS: The common chromosomal alterations included gains of chromosomes 1q, 2, 3q, 7q,8q,12 as well as losses of chromosomes 3p, 9p, 11q, 16q. CONCLUSION: There are multiple regions of chromosomes with copy number changes in NPC. The tumor suppressor genes and oncogenes on these regions may be involved in the development and progress of nasopharyngeal carcinoma.

Chromosome Aberrations↗
PubMed2001

[Design and implementation of mini PACS in the hospital].

This paper introduces the principle and classification of picture archiving and communication system (PACS) and some pertinent technologies, including the capture methods of pictures data, images storage equipment and the choice of medium, and the network of images transmission. Also presented is an analysis of the merit and demerit of different kinds of PACS, and in the end we put forward a PACS design method and network framework based on practical implication in the Jinwei project experimental hospital.

Computer Communication Networks↗
PubMed2001

[The effect of composite Radix Salviae Militiorrhizae injection on myocyte apoptosis in acute myocardial infarction of rabbits].

OBJECTIVE: To study the effect of composite Radix Salviae Militiorrhizae injectiion on myocyte apoptosis in experimental acute myocardial infarction of rabbits. METHODS: The models of acute myocardial infarction were made with rabbits. The method of terminal deoxynucleotidyl transferase mediated dUTP nick end labeling was used to explore myocardial cell apoptosis in different injury area of acute myocardial infarction. The activities of plasma superoxide dismurase and the contents of serum malonic aldehyde were determined. RESULTS: When rabbits were in the condition of acute myocardial infarction, there were myocyte apoptosis in the area of ischemia reperfusion injury and the area of infarction. The number of myocyte apoptosis and the myocyte rate in control group and composite Radix Salviae Militiorrhizae injectiion treated group were higher than in sham-operated group (P < 0.001). In the area of ischemia reperfusion injury the number of myocyte apoptosis and the myocyte rate in composite salvia militiorrhiza-treated group were lower than in control group (P < 0.05). The activity of plasma superoxide dismurase in composite salvia militiorrhiza-treated group was higher than in control group (P < 0.05), but lower than in sham-orerated group (P < 0.001). The content of serum malonic aldehyde in composite Radix Salviae Militiorrhizae injectiion treated group were lower than in control group (P < 0.05), but higher than in sham-operated group (P < 0.01). CONCLUSION: Composite Radix Salviae Militiorrhizae injectiion could reduce the number of myocyte apoptosis in the area of ischemia reperfusion injury, which maybe explained by the increase of the activity of plasma superoxide dismurase and the decrease of the contents of serum malonic aldehyde.

Animals↗
PubMed2001

[Appraisal and analysis of heat removing characteristic of liquid cooling garment using thermal manikin].

OBJECTIVE: To analyze the relation of the design parameters and appraise the heat removing characteristic of liquid cooling garment (LCG) using thermal manikin. METHOD: To appraise the design of LCG, the thermal manikin wearing LCG and heat insulation garment was put in the temperature cabin, then the inlet and outlet temperature of cooling liquid in the LCG were measured and the heat removed was calculated. RESULT: The relationship between the design parameters and heat removed, and also that between the design parameters and temperature ratio efficiency were found. And the heat removed was calculated. It provided a reasonable basis for appraisal of LCG. CONCLUSION: The design of the LCG for the EVA space suit was found to be reasonable. The flow rate regulation range was too narrow, and the change of heat removing capacity depended on the change of inlet temperature.

Body Temperature Regulation↗
PubMed2001

[Establishment and characterization of a cell line derived from human ovarian mucinous cystadenocarcinoma].

OBJECTIVE: To establish a cell line of human ovarian cancer, and study its characterization. METHODS: The cell line was established by the cultivation of subsides walls, and kept by freezing. The morphology was observed by microscope and electromicroscope. The authors studied its growth and propagation, the agglutination test of phytohemagglutinin (PHA), the chromosome analysis, heterotransplanting, immuno-histochemistry staining, the analysis of hormone, the pollution examination and the test of sensitivity to virus etc. RESULTS: A new human ovarian carcinoma cell line, designated ovarian mucinous cystadenocarcinoma 685 (OMC685), was established from mucinous cystadenocarcinoma. This cell line had subcultured to 91 generations, and some had been frozen for 8 years and revived, still grew well. This cell line possessed the feature of glandular epithelium cancer cell. The cells grew exuberantly, and the agglutinating test of PHA was positive. Karyotype was subtriploid with distortion. Heterotransplantations, alcian blue periobic acid-schiff (AbPAS), mucicarmine, alcian blue stainings, estradiol (E2) and progesterone were all positive. Without being polluted, it was sensitive to polivirus-I, adenovirus 7 and measles virus. CONCLUSIONS: OMC685 is a distinct human ovarian tumous cell line.

Cell Count↗
PubMed2001

Interleukin-6 in bronchoalveolar lavage fluid from patients with COPD.

OBJECTIVE: To obtain new insights into the behavior of Interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) and released from alveolar macrophages (AM) in chronic obstructive pulmonary diseases (COPD), and reveal the relationship between IL-6 and the development of emphysema in COPD. METHODS: IL-6 in BALF and released by AM in BALF were examined in 7 non-smoking subjects and 21 patients with COPD. According to the 95% confidence limits of IL-6 in BALF from non-smoking subjects, the patients were divided into two groups: those who were within the limits were assigned to the first group, and those who were above the limits were assigned to the second group. RESULTS: The concentration of IL-6 released by AM was much higher in the second group than in the first one. Between the two groups, significant differences were found in pulmonary function. CONCLUSION: Our results suggest that the concentration of IL-6 released by AM may be related with pulmonary function, and IL-6 may play a role in the development of emphysema in patients with COPD.

Bronchoalveolar Lavage Fluid↗
PubMed2001

[The influence of dexamethasone on mRNA expression of interleukin-5, interleukin-3 and granulocyte-macrophage colony-stimulating factor receptors in eosinophils of guinea pig asthma model].

OBJECTIVE: To investigate the iufluence of dexamethasone (DM) on expression of interleukin-5 receptor alpha(IL-5R alpha), interleukin-3 receptor alpha (IL-3R alpha), granulocyte-macrophage-colony-stimulating factor receptor alpha(GM-CSFR alpha) and common beta receptor (beta cR) in bronchalveolar lavage fluid (BALF) eosinophils in guinea pig asthma model, and to study the mechanism of promotion effect of steroid on eosinophil apoptosis. METHODS: 18 guinea pigs were divided into three groups randomly, normal control group, asthma group and DM-treated group. Asthma models of guinea pig were sensitized by ovalbumin(OVA). Hypodense EOS (HEOS) and normodense EOS (NEOS) were purified from BALF by gradients of percoll. Apoptosis was detected by method of TdT-mediated dUTP nick end labeling. The mRNA expression of IL-5R alpha, IL-3R alpha, GM-CSFR alpha and common beta receptor (beta cR) in eosinophils were measured by RT-PCR and in situ hybridization. RESULTS: Apoptosis of HEOS and NEOS in asthma group(4.0 +/- 2.0 and 3.0 +/- 2.0, respectively) were significanteylower than normal group(8.0 +/- 2.0 and, 7.0 +/- 2.0 respectively, P < 0.01), while the numbers of EOS cells (75.2 +/- 12.6 and 50.7 +/- 11.2, respectively) were significantly higher than normal group (4.8 +/- 1.5 and 9.5 +/- 2.6, respectively, P < 0.01). The BALF HEOS and NEOS of asthma group were remarkably reduced after DM treatment (14.8 +/- 8.0 and 20.0 +/- 7.0, respectively, P < 0.01), Eosinophils apoptosis in DM-treated group (24.0 +/- 5.0 and 22.0 +/- 4.0, respectively) was remarkably higher than those in asthma group (P < 0.01); The expressions of IL-5R alpha, IL-3R alpha, GM-CSFR alpha mRNA of BALF EOS of asthma group were significantly lower, but the expression of beta cR mRNA was significantly higher than those of normal group(P < 0.01, 0.05). The expressions of IL-5R alpha, IL-3R alpha, GM-CSFR alpha mRNA in EOS of DM-treated group were significantly higher, while the expression of beta cR mRNA were significantly lower than those of asthma group (P < 0.01, 0.05). CONCLUSIONS: DM promote the apoptosis of BALF EOS of guinea pigs asthma models. and reduce EOS infiltration in lung of guinea pigs asthma models. It also decreased the effect of IL-5, IL-3 and GM-CSF and promoted the apoptosis of lung EOS through inducing the mRNA expression of their specific alpha chain and reducing the mRNA expression of the common beta chain.

Animals↗
PubMed2001

[Emission of volatile sulfur gases from Chinese paddy soils].

In the paper, emission of volatile sulfur gases from paddy soil was discussed in a growth period of paddy rice by constructing a field sampling system. The result showed that COS, CS2, DMS and DMDS were mainly emitted from paddy soil. The order of emission fluxes was 81.11, 6.33 and 10.71 mg.(m2.a)-1. Sulphur emission fluxes of Chinese paddy soil was 0.013662 Tg/a, and those of world paddy soil was 0.07992 Tg/a.

Oryza↗
PubMed2001

[Pretreatment of benzoic acid wastewater].

The benzoic acid wastewater produced from toluene by liquid-phase oxidation is one of the difficult wastewater to treat because of multi-component, high CODcr value (20,000-100,000 mg/L) and nonbiodegradability. A pretreatment way of this wastewater by extraction based on chemical complexation was carried out, and tributyl phosphate (TBP), trialkylamine (7301) and trialkyphosphine oxide (TRPO) were used as complexing agent with n-octanol, kerosene as cosolvent and diluent respectively. Results showed that benzoic acid was recovery successfully and the CODcr value of wastewater was reduced to 1/6 of the initial value through multi-stages cross-flow extraction. Back-extraction was quite easy and the solvent could be reused.

Benzoic Acid↗
PubMed2001

[Surgical correction of hemimandibular hyperplasia].

OBJECTIVE: Evaluate the surgical corrective result of hemimandibular hyperplasia. METHODS: 33 patients were treated by orthognathic surgical procedures. The affected condyles of all patients were removed. RESULTS: The surgical corrective result of all patients was satisfactory. CONCLUSIONS: The deformity can be properly corrected by orthognathic surgical procedures. In order to prevent the reoccurrence of the deformity and acquire good corrective result, it is essential to remove the affected condyle and to parallel the occlusal canting.

Adolescent↗